Instrumentation for Research in Animal-Cell Culture and Metabolic Engineering

本提案中要求的仪器将增强 动物细胞培养工程中PI研究 厌氧细菌的代谢工程， PI更密切地监测细胞代谢的变化， 产品表达 所要求的文书将是 最初用于三个主要研究项目。 第一 项目调查了各种环境影响 强调通过杂交瘤细胞生产单克隆抗体。 它还将研究应激蛋白启动子的效用 用于通过动物细胞生产重组糖蛋白。 第二部分考察了生物反应器流体动力学的影响 环境对生存力、代谢、大分子 动物细胞的合成和产物表达， 微载体和自由悬浮反应器。 第三个项目 将研究改变主要 丁酸厌氧代谢（代谢工程） 细菌（梭菌），以构建更有效的 用于大规模厌氧的全细胞生物催化剂 发酵

The instrumentation requested in this proposal will enhance the PIs research in animal-cell culture engineering and metabolic engineering of anaerobic bacteria by enabling the PIs to more closely monitor changes in cell metabolism and product expression. The requested instruments will be initially used for three major research projects. The first project investigates the effect of various environmental stresses on monoclonal antibody production by hybridoma cells. It will also examine the utility of stress-protein promoters for production of recombinant glycoproteins by animal cells. The second examines effects of the bioreactor hydrodynamic environment on the viability, metabolism, macromolecular synthesis, and product expression of animal cells in microcarrier and free-suspension reactors. The third project will investigate the ability and means of altering the primary metabolism (metabolic engineering) of butyric-acid anaerobic bacteria (clostridia) in order to construct more efficient whole-cell biocatalysts for large scale anaerobic fermentations.