Multilibrary Strategies for Developing Complex Transgenic Microbial Phenotypes

本项目旨在开发新的基于多文库的方法来产生复杂的表型，这些方法将有助于为许多生物处理应用开发具有工业重要性的菌株，例如那些目前在生物处理或可再生资源(绿色技术)或生物修复中目前使用或预期使用的菌株。此外，该项目产生的知识将对乙醇耐受的基础生物学做出重大贡献。更广泛的影响：这个项目？S的策略可以应用于任何理想的有机体和生物加工应用，其中需要一个可选择的复杂表型，特别是可再生资源的利用过程。在过去几个月里，利用可再生资源生产化学品和生物燃料的高效和经济可行的技术的重要性已成为国家和国际的一个紧迫优先事项。这是由几个重要的事实驱动的。首先，石油等不可再生能源的枯竭，加上快速发展的经济体和世界人口推动的能源需求增加，导致石油和能源价格居高不下。第二个重要的事实是，人们意识到，不可再生的化石燃料会导致重大的气候变化，这可能会导致灾难性的天气模式变化。相比之下，生物质是一种碳中性的可再生资源，用于生产生物燃料和化学品。最后，该项目为实验基因组学和相关的高通量技术的学生教育和培训提供了独特的机会。

CBET-0756451PapoutsakisIntellectual Merit This project aims to develop novel multi-library-based approaches for generating complex phenotypes that would be useful for developing industrially important strains for many bioprocessing applications, such as those currently employed or anticipated in the bioprocessing for the utilization or renewable resources (green technologies) or in bioremediation. In addition, the knowledge generated from this project will make a significant contribution to the basic biology of ethanol tolerance. Broader Impact: This project?s strategies can be applied to any desirable organism and bioprocessing application whereby a selectable complex phenotype is desirable, and especially processes for the utilization of renewable resources. The importance of efficient and economically viable technologies for production of chemicals & biofuels from renewable resources has become an urgent national and international priority over the last few months. This was driven by several important facts. First, the depletion of non-renewable energy sources, such as oil, coupled with increased energy demand driven by fast developing economies and world population has led to high oil and energy prices. And a second important fact is the realization that non-renewable fossil fuels lead to significant climatic changes that may lead to catastrophic weather-pattern changes. In contrast, biomass is a carbon-neutral renewable resource for production of biofuels and chemicals. Finally, this project provides unique opportunities for student education and training in experimental genomics, and associated high-throughput technologies.