ME: Metabolic Engineering of Solvent Tolerance in Anaerobic Bacteria

ME：厌氧细菌溶剂耐受性的代谢工程

• 批准号: 9911231
• 负责人: Eleftherios Papoutsakis
• 金额: $ 34.42万
• 依托单位: Northwestern University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9911231&HistoricalAwards=false
• 关键词: ME; Metabolic; Engineering; Solvent; Tolerance

The objective of this project is to understand the molecular basis of solvent tolerance in Clostridium acetobutylicum, with the purpose of using this information to obtain strains that produce higher concentrations of solvents. The research is organized around two hypotheses: (1) that the ability to produce high concentrations of solvent (butanol), and also butanol tolerance, is enhanced by increased protein stability in strains that overexpress heat-shock proteins; and (2) that butanol tolerance is enhanced by expression of native solvent/drug resistance genes, e. g. proteins of the multidrug efflux family. This project is being jointly funded by two programs at NSF and by the Environmental Protection Agency (EPA).

本项目的目的是了解乙酰丁酸梭菌耐溶剂性的分子基础，目的是利用这些信息来获得产生更高浓度溶剂的菌株。这项研究围绕两个假设展开：(1)过量表达热休克蛋白的菌株通过提高蛋白质稳定性来增强产生高浓度溶剂(丁醇)的能力，以及丁醇耐受性；(2)通过表达天然的溶剂/耐药基因，例如多药外排家族的蛋白质，可以增强丁醇耐受性。该项目由美国国家科学基金会和美国环境保护局(EPA)的两个项目联合资助。