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Collaborative Research: Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia

Collaborative Research: Genetic Analysis and Metabolic Engineering of Solventogenic Clostridia
合作研究：产溶剂梭菌的遗传分析和代谢工程
批准号：
9905669
负责人：
Eleftherios Papoutsakis
金额：
$81.83万
依托单位：
Northwestern University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2000
资助国家：
美国
项目状态：
已结题
起止时间：
2000-04-01 至 2004-03-31
来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9905669&HistoricalAwards=false
关键词：
CollaborativeResearchGeneticAnalysisMetabolic

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英文摘要
The objective of the project is to investigate the metabolic engineering of solventogenic Clostridia using modern molecular biology and genetic techniques. The primary goal of this interdisciplinary proposal is to genetically alter the metabolism of Clostridium acetobutylicum. The proposal describes four areas of activity; (1) a detailed examination of the regulation of the sol locus genes through a putative repressor, SolR; (2) a molecular analysis of the function of sporOA and of ORF (orfY) gene products, both of which are likely to be important in the expression of the sol locus genes; (3) an analysis of patterns of gene expression using cDNA arrays; and (4) the further development of antisense RNA as a metabolic engineering tool.
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