Complete Reductive Dechlorination of Trichloroethylene (TCE) by non-Dehalococcoides Microorganisms

摘要CBET-0853835 Finneran，Kevin TUniversity of Illinois at Urbana-Champaign通过非脱卤球菌属微生物对三氯乙烯（TCE）进行完全还原脱氯拟议工作的目标是：1）证明Dehalococcoides属以外的生物可以完全将TCE还原为乙烯，2）使用开发的富集培养物来表征代谢，地球化学，和非脱卤球菌属微生物的氯化溶剂生物降解动力学，3）证实优势非脱卤球菌属微生物与脱硫孢子菌属内的生物最密切相关，4）表征在完全脱氯期间乙酸盐氧化和利用的途径，以确定其是否作为唯一电子供体被直接氧化（通过脱卤球菌属或非脱卤球菌属液体富集培养物）或定义其在相同条件下可产生氢的机制，5）证明电子供体当与过量电子供体添加相比时，以相对于存在的电子受体的化学计量浓度添加的（乙酸盐或H2）促进更快的TCE还原成乙烯，（6）对Fe（III）还原和完全脱氯同时进行的具体条件进行了量化，这是首次研究非Dehalococcoides完全脱氯的可能性。这将改变基础和应用科学和工程方面的halorespiration作为一种代谢过程，氯化溶剂生物修复作为一种实用技术。此外，这项工作将表征几个知之甚少的环境过程，包括重叠的Fe（III）还原和完全脱氯，和乙酸盐作为直接电子供体的卤呼吸。该提案已被构建的方式，所有的假设是相关的和独立的，所产生的数据将有利于更广泛的科学界研究基础halorespiration科学或那些调查应用生物修复的TCE.The拟议的推广计划包括学生谁是代表性不足的群体在环境工程的成员，和从业者谁将最终受益于数据。这些学生将包括研究生和本科生，并将专门从少数民族和女学生项目中招收。实践者方面将最终与一个已经表示对工作感兴趣的团体直接接触，并通过会议和在其区域办事处向小团体进行个人介绍与其他团体直接接触。整个研究人员，包括拟议的博士后学者将与所有从业者进行互动，使他们习惯于实验室到现场的研究过渡，以及环境工程实践的务实方面如何影响基础研究科学。

ABSTRACTCBET-0853835Finneran, Kevin TUniversity of Illinois at Urbana-ChampaignComplete Reductive Dechlorination of Trichloroethylene (TCE) by non-Dehalococcoides MicroorganismsThe objectives of the proposed work are to: 1) demonstrate that organisms outside of the genus Dehalococcoides can completely reduce TCE to ethene, 2) use the enrichment cultures developed to characterize the metabolism, geochemistry, and chlorinated solvent biodegradation kinetics of non-Dehalococcoides microorganisms, 3) verify that the dominant non-Dehalococcoides microorganism(s) are most closely related to organisms within the genus Desulfosporosinus, 4) characterize the pathway for acetate oxidation and utilization during complete dechlorination to determine if it is directly oxidized as the sole electron donor (by Dehalococcoides or non-Dehalococcoides liquid enrichment cultures) or to define the mechanisms by which it may generate hydrogen under the same conditions, 5) demonstrate that electron donors (acetate or H2) added at stoichiometric concentrations relative to the electron acceptors present promotes faster TCE reduction to ethene when compared to excess electron donor addition, and 6) quantify the specific conditions in which Fe(III) reduction and complete dechlorination are promoted simultaneously.This is the first research to address the probability of non-Dehalococcoides complete dechlorination. This will change fundamental and applied science and engineering with respect to halorespiration as a metabolic process, and chlorinated solvent bioremediation as a practiced technology. In addition, the work will characterize several poorly understood environmental processes including the overlap of Fe(III) reduction and complete dechlorination, and acetate as a direct electron donor for halorespiration. The proposal has been constructed in a manner that all hypotheses are relevant and independent, and the data generated will benefit the broader scientific community studying basic halorespiration science or those investigating applied bioremediation of TCE.The proposed outreach plan incorporates both students who are members of underrepresented groups in environmental engineering, and practitioners who will ultimately benefit from the data. The students will be both graduate level and undergraduate level, and will be recruited specially from programs for minority and female students. The practitioner aspect will culminate in direct contact with one group who has already expressed interest in the work and with others via conferences and individual presentations to small groups at their regional offices. Entire research staff including the proposed postdoctoral scholar will interact with all practitioners such that they become accustom to the lab to field transition for research, and how the pragmatic aspects of environmental engineering practice can influence fundamental research science.