Brain Mimetic Materials for Cancer Cell Migration Studies

用于癌细胞迁移研究的类脑材料

• 批准号: 0854015
• 负责人: Jessica Winter
• 金额: $ 30万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-15 至 2012-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0854015&HistoricalAwards=false
• 关键词: Brain; Mimetic; Materials; Cancer; Cell

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5)0854015WinterThis research objective of this project is to create new brain mimetics and migration models to explore the effect of the extracellular matrix (ECM) on glioma cell migration. The mimetics consist of hydrogels; swollen, cross-linked, yet insoluble, polymer networks, composed of hyaluronic acid and proteoglycans (e.g., brevican and versican). Mimetic materials will be used to construct two three-dimensional models of in vitro cell migration: an invasion assay, which examines migration of cells from solution into a 3D brain tissue mimetic; and in interface assay, which examines migration of cells from a 3D brain mimetic of one composition into a brain mimetic of differing composition. These models differ from current methods of investigating tumor cell migration because they are (a) composed of materials that naturally occur in the brain, (b) are three-dimensional, more closely mimicking in vivo conditions. Deliverables include brain tissue mimetic materials, models of cell migration, preliminary analysis of cell response to two types of brain tissue mimetic materials (i.e., 'cancer' brain with added brevican, 'normal' brain with added versican), engineering student education at the graduate and undergraduate level, and a hallway cart exhibit based on this research for COSI, a local science museum.The research will provide new tools with the potential to transform understanding of glioma cancer cell migration. Gliomas, tumors of supporting cells in the brain, are among the most deadly cancers, with an average survival of 10-30 months from time of diagnosis, and affect 22,500 individuals/year. They are particularly difficult to treat because they are highly aggressive, infiltrating throughout the brain. In addition to clinical benefits, this work will enhance research infrastructure, creating new collaborations between engineering and clinical departments. Research results will be disseminated through journal publications, conference presentations, and also, to the general public, through interactions with an area science museum. Graduate and undergraduate students will participate in the research, and results will be disseminated through classroom materials as well.

该项目的研究目标是创建新的大脑模拟和迁移模型，以探索细胞外基质（ECM）对胶质瘤细胞迁移的影响。模拟物由水凝胶组成；膨胀的，交联的，但不溶的聚合物网络，由透明质酸和蛋白聚糖（例如，灯尾草聚糖和花蜜聚糖）组成。模拟材料将用于构建体外细胞迁移的两个三维模型：入侵试验，检查细胞从溶液迁移到三维脑组织模拟物；以及界面测定，其检查细胞从一种成分的3D脑模拟物向不同成分的脑模拟物的迁移。这些模型不同于目前研究肿瘤细胞迁移的方法，因为它们(a)由大脑中自然存在的物质组成，(b)是三维的，更接近于模拟体内条件。交付成果包括脑组织模拟材料、细胞迁移模型、细胞对两种类型脑组织模拟材料（即，添加布雷维肯的“癌症”大脑，添加维肯的“正常”大脑）的反应的初步分析、研究生和本科生水平的工程学生教育，以及基于该研究为COSI（当地科学博物馆）举办的走廊推车展览。这项研究将提供新的工具，有可能改变对胶质瘤癌细胞迁移的理解。胶质瘤是大脑中支持细胞的肿瘤，是最致命的癌症之一，自诊断起平均生存期为10-30个月，每年影响22500人。它们尤其难以治疗，因为它们具有很强的侵袭性，可以渗透到整个大脑。除了临床效益外，这项工作还将加强研究基础设施，在工程和临床部门之间建立新的合作关系。研究成果将通过期刊出版物、会议报告以及通过与地区科学博物馆的互动向公众传播。研究生和本科生将参与研究，研究结果也将通过课堂材料传播。