Fluorescent-Magnetic Nanomanipulators for Cytoskeletal Mechanical Investigations

用于细胞骨架力学研究的荧光磁性纳米操纵器

• 批准号: 0900377
• 负责人: Jessica Winter
• 金额: $ 31.34万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0900377&HistoricalAwards=false
• 关键词: Fluorescent; Magnetic; Nanomanipulators; Cytoskeletal; Mechanical

Fluorescent-Magnetic Nanomanipulators for Cytoskeletal Mechanical InvestigationsAbstractThe research objective of this award is to develop a new methodology to examine the role of mechanical force in cell migration. The approach consists of fluorescent-magnetic nanomanipulators that impart force to the cell?s internal scaffolding (i.e., the cytoskeleton) through stimulation by custom-designed magnetic instrumentation. Cell response is observed via the nanomanipulators? fluorescence property. This research will focus on examining migration of cells crucial to the wound healing process (i.e., fibroblasts). Fluorescent-magnetic nanomanipulators will be characterized and optimized to interact with magnetic instrumentation; proof of concept of nanomanipulator delivery to the cell interior and manipulation potential will be demonstrated; and the effect of force applied by nanomanipulators on cell migration will be evaluated in fibroblasts. Deliverables include optimized nanomanipulators and magnetic instrumentation, validation of the intracellular force application method, documentation of research results in the fibroblast model, engineering student education at the graduate and undergraduate level, and a module based on this research for public school outreach disseminated by COSI, a local science museum.If successful, this research will provide new tools to explore the influence of mechanical force on protein expression and cellular processes, in particular those that underlie cell migration. Cell migration is a critical component of several biological events including wound healing, fetal development, and cancer metastasis. This research would provide methods to apply force to subcellular components with the smallest manipulators yet studied (~ 10-50 nm), sizes that are similar to many of the components of interest (e.g., protein receptor, cytoskeletal element). Research results will be disseminated through journal publications, conference presentations, and also, to the general public, through interactions with an area science museum. Graduate and undergraduate students will participate in the research, and results will be disseminated through classroom materials as well.

本奖项的研究目标是开发一种新的方法来研究机械力在细胞迁移中的作用。该方法包括荧光磁性纳米操纵器，赋予力的细胞？S内部支架（即，细胞骨架）通过定制设计的磁性仪器的刺激。通过纳米操纵器观察细胞反应？荧光性质。这项研究将集中在检查对伤口愈合过程至关重要的细胞的迁移（即，成纤维细胞）。磁纳米操纵器将被表征和优化，以与磁性仪器相互作用;纳米操纵器递送到细胞内部和操纵潜力的概念证明将被证明;纳米操纵器施加的力对细胞迁移的影响将在成纤维细胞中进行评估。可获得的成果包括优化的纳米操纵器和磁性仪器、细胞内力施加方法的验证、成纤维细胞模型中的研究结果文档、研究生和本科生水平的工程学生教育以及基于该研究的由当地科学博物馆COSI传播的公立学校外展模块。这项研究将为探索机械力对蛋白质表达和细胞过程的影响提供新的工具，特别是那些构成细胞迁移基础的过程。细胞迁移是包括伤口愈合、胎儿发育和癌症转移在内的若干生物学事件的关键组成部分。这项研究将提供方法来施加力到亚细胞组件与最小的操纵器尚未研究（~ 10-50 nm），大小类似于许多感兴趣的组件（例如，蛋白受体、细胞骨架元件）。研究成果将通过期刊出版物，会议演示，并通过与地区科学博物馆的互动向公众传播。研究生和本科生将参与研究，研究结果也将通过课堂材料传播。