Magnetic-Fluorescent Bifunctional Nanoparticles for Biomedical Applications

用于生物医学应用的磁荧光双功能纳米颗粒

• 批准号: 0907204
• 负责人: Yuping Bao
• 金额: $ 22.05万
• 依托单位: University of Alabama Tuscaloosa
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0907204&HistoricalAwards=false
• 关键词: Magnetic; Fluorescent; Bifunctional; Nanoparticles; Biomedical

TECHNICAL SUMMARYThe novelty of this proposal lies in: (1) Fluorescent Ag nanoclusters (a few Ag atoms) are used as the fluorescent component, which can overcome the size increase issue of the current practice. (2) The capping agents of iron oxide nanoparticles are directly used as templates for Ag nanocluster formation under UV-radiation without the addition of other chemicals, which avoids tedious synthetic procedures. Besides the routine structural and physicochemical characterization of the proposed integrated nanostructures, detailed photophysical properties of Ag nanoclusters on nanoparticle surfaces will be explored with fluorescent correlation spectroscopy. Further, the structural integrity of the integrated nanostructures will be tested in biological systems. The proposed work will offer fundamental insight into the integration of multiple nanocomponents (e.g., correlations between structure-properties-performance).NON TECHNICAL SUMMARYMagnetic nanoparticles have significantly advanced cancer treatments through targeted drug delivery and localized therapy and further make simultaneous therapy and diagnosis possible as magnetic resonant imaging (MRI) contrast agents. Unfortunately, these applications are limited by the expensive MRI equipments, which are not available to common research laboratories. Currently, fluorescence imaging remains the primary choice for bio-imaging because of its high sensitivity. This proposal will develop magnetic-fluorescent nanoparticles which provide a single platform with therapeutic and diagnostic functions. Beyond the new possibilities of this nanostructure in biomedical fields, further findings on the photophysics of Ag nanoclusters on nanoparticle surfaces may provide valuable information to physicists and spectroscopists. The integrated nanostructures may be used for monitored magnetic removal of the contaminants from the environment. Part of the research project will be incorporated in the junior summer laboratory course (ChBE320), a five hour laboratory operation class, promoting students? learning through hands-on experience, such as building alternating current (AC) magnetic coils to study the heat generation from magnetic nanoparticles and creating magnetic-fluorescent nanoparticle arrays on a chip to demonstrate the bio-sensing capability.

本发明的新奇在于：（1）使用荧光Ag纳米团簇（少量Ag原子）作为荧光组分，这可以克服当前实践中的尺寸增加问题。(2)氧化铁纳米颗粒的封端剂直接作为模板，在紫外光照射下形成银纳米团簇，无需添加其他化学物质，避免了繁琐的合成步骤。除了常规的结构和物理化学表征的建议集成的纳米结构，详细的纳米粒子表面上的银纳米团簇的物理性质将探索与荧光相关光谱。此外，集成纳米结构的结构完整性将在生物系统中进行测试。拟议的工作将提供对多个纳米组件（例如，非技术概述磁性纳米颗粒通过靶向药物递送和局部治疗显著地改进了癌症治疗，并且作为磁共振成像（MRI）造影剂进一步使同时治疗和诊断成为可能。不幸的是，这些应用受到昂贵的MRI设备的限制，这些设备不适用于普通的研究实验室。目前，荧光成像仍然是生物成像的主要选择，因为它的高灵敏度。该提案将开发磁性荧光纳米颗粒，提供具有治疗和诊断功能的单一平台。除了这种纳米结构在生物医学领域的新可能性之外，纳米颗粒表面上Ag纳米团簇的电子物理学的进一步发现可能为物理学家和光谱学家提供有价值的信息。集成纳米结构可用于从环境中监测磁性去除污染物。部分研究项目将纳入初级夏季实验室课程（ChBE 320），这是一门五小时的实验室操作课程，促进学生？通过实践经验进行学习，例如构建交流（AC）磁性线圈以研究磁性纳米颗粒产生的热量，并在芯片上创建磁性荧光纳米颗粒阵列以展示生物传感能力。