Active Cell Culture Using Surface Shape Memory

使用表面形状记忆进行活性细胞培养

• 批准号: 0907578
• 负责人: James Henderson
• 金额: $ 39.94万
• 依托单位: Syracuse University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0907578&HistoricalAwards=false
• 关键词: Active; Cell; Culture; Using; Surface

ID: MPS/DMR/BMAT(7623) 0847578 PI: Henderson, James ORG: SyracuseTitle: Active Cell Culture Using Surface Shape Memory This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).INTELLECTUAL MERIT: The goal of this proposal is to create and understand active cell culture substrates using surface shape memory and, in parallel, to study unique properties of shape memory polymers (SMPs) that promise broad application in biological and biomedical fields. To achieve this goal, a series of coordinated objectives will be pursued. SMP material characteristics will be tuned by systematically varying temperature transition, elasticity, and soft-elasticity plateau. The micron-scale viscoelastic constitutive behavior of SMPs of varying phase composition, orientation, and crosslink density will be determined using a new lateral force indentation method. The biocompatibility and amenability to cell culture of SMPs will be rigorously evaluated using the agar diffusion assay to quantify cytotoxicity, the MTS tetrazolium colorimetric assay to quantify cell attachment kinetics, and measurement of modulus, gel fraction, phase transition temperature, and microstructure to analyze long term stability in an aqueous environment. Methods for preparing surface SMPs that transition from a flat surface to a complex surface geometry, and vice versa, will be studied by employing processes that prescribe equilibrium shapes then fix temporary shapes with embossing. Complex surface geometries for application in active cell culture will be designed following analysis of established, bioactive surface geometries. Methods for cell-compatible thermocycling of liquid crystalline elastomer substrates and in situ imaging of the cell response will be developed using a heated stage and reflected light microscopy. Lastly, proof-of-concept active cell culture substrates with specific target uses, such as directing cell lineage specification, stretching cells, assaying cell traction forces, and orienting and patterning fields of homogeneous cells or heterogeneous cells, will be developed.BROADER IMPACTS: The goal of the proposal is to create a new multimedia program, entitled At the Interface, to educate the public about research at the biology/engineering interface. The program will target high-school students and the non-technical public while also providing a useful entry point for scientists and engineers who may want to proceed to more technical assessment of the topics covered. Biomaterials will frequently be used as a theme, but diverse topics from all areas of the research interface will be addressed. A collaborating team of students and faculty from the Syracuse S. I. Newhouse School of Public Communications has been assembled to add to the expertise of the PI and co-PI in the areas of magazine, newspaper, and online journalism and television, radio, and film journalism. The team will achieve its goal by pursuit of the following focused objectives: (1) Produce short videos showcasing current research at the interface of biology and engineering to be hosted on the website of the Syracuse Biomaterials Institute. (2) Produce brief (5 min) educational radio spots that report on technological advances at the interface for broadcast on the central New York NPR affiliate, WRVO. (3) Develop short text-based summaries of all video and radio pieces to be available in the form of press releases in English and Spanish. (4) Develop and deploy metrics for assessing the penetration of the video, radio, and text platforms in the community and the resulting impact on public understanding. (5) Study the potential for the video and radio platforms to be expanded to include more content and a larger audience.

ID：MPS/DMR/BMAT（7623）0847578 PI：亨德森，詹姆斯 ORG：SyracuseTitle：该奖项由2009年美国复苏和再投资法案（Public Law 111-5）资助。智力优势：该提案的目标是利用表面形状记忆创建和理解活性细胞培养基质，同时研究形状记忆聚合物（SMP）的独特特性，这些特性有望在生物和生物医学领域得到广泛应用。 为实现这一目标，将努力实现一系列协调一致的目标。 SMP材料特性将通过系统地改变温度转变、弹性和软弹性平台来调整。将使用一种新的横向力压痕法来确定不同相组成、取向和交联密度的SMP的微米级粘弹性本构行为。将使用琼脂扩散试验来量化细胞毒性，使用MTS四氮唑比色试验来量化细胞附着动力学，并测量模量、凝胶分数、相变温度和微观结构来分析长期稳定性，严格评估SMPs的生物相容性和对细胞培养的适应性在水性环境中。制备从平坦表面过渡到复杂表面几何形状的表面SMP的方法，反之亦然，将通过采用规定平衡形状然后用压花固定临时形状的工艺来研究。复杂的表面几何形状的应用程序在活性细胞培养将设计以下分析建立，生物活性表面几何形状。将开发用于液晶弹性体基板的细胞相容性热循环和细胞响应的原位成像的方法，使用加热台和反射光显微镜。最后，将开发具有特定目标用途的概念验证活性细胞培养基质，例如指导细胞谱系特化、拉伸细胞、测定细胞牵引力以及同质细胞或异质细胞的定向和图案化领域。该提案的目标是创建一个新的多媒体程序，题为在界面，教育公众关于生物学/工程学接口的研究。该计划将针对高中学生和非技术公众，同时也为科学家和工程师提供了一个有用的切入点，他们可能希望对所涵盖的主题进行更多的技术评估。生物材料将经常被用作一个主题，但从研究界面的所有领域的不同主题将得到解决。 一个由来自锡拉丘兹大学的学生和教师组成的合作小组。I.公共传播的纽豪斯学校已经组装，以增加在杂志，报纸和在线新闻和电视，广播和电影新闻领域的PI和共同PI的专业知识。该团队将通过追求以下重点目标来实现其目标：（1）制作短视频，展示生物学和工程学接口的当前研究，并将其托管在锡拉丘兹生物材料研究所的网站上。(2)制作简短的（5分钟）教育广播节目，报告在中央纽约NPR分支机构WRVO的广播接口的技术进步。 (3)制作所有录像和广播节目的简短摘要，以英文和西班牙文新闻稿的形式提供。(4)开发和部署用于评估视频、广播和文本平台在社区中的渗透率以及对公众理解的影响的指标。(5)研究扩大视频和广播平台的潜力，以包括更多的内容和更多的受众。