Collaborative Research: Chemometrics and Comprehensive Two-Dimensional Liquid Chromatography: Toward Achieving the Promise for Metabolomics

弗吉尼亚联邦大学（VCU）的Sarah Rutan教授和明尼苏达大学的Peter卡尔教授得到了化学系分析和表面化学项目的支持，以开发二维液相色谱的定量方面，重点是代谢组学中的应用。具体而言，这项工作解决了分离条件的优化和新的手段，以加速第二维分离和分析所得到的数据的探索。其目的是将一个耗时的半定量过程转化为一种快速有效的方法，为生物标志物鉴定等应用提供精确的定量结果。如果成功，这项工作将为生物科学家提供重要的新工具，并将有助于提高对复杂生物系统的理解。为了使更广泛的受众更容易了解代谢组学的概念，将开发一个网站，其中有代谢和分离的在线模拟器，沿着设计用于高中课堂的相关活动。

Professors Sarah Rutan at Virginia Commonwealth University (VCU) and Peter Carr at the University of Minnesota are supported by the Analytical and Surface Chemistry Program in the Division of Chemistry to develop quantitative aspects of two-dimensional liquid chromatography, with a focus on applications in metabolomics. Specifically, this work addresses the optimization of the separation conditions and exploration of novel means to accelerate both the second dimension separation and the analysis of the resulting data. The aim is to transform a time-consuming process that is semi-quantitative at best into a rapid and efficient method that will provide precise quantitative results for applications such as biomarker identification. If successful, the work will provide important new tools for biological scientists and will contribute towards improved understanding of complex biological systems. In order to make the concepts of metabolomics more accessible to a broader audience, a website with online simulators for metabolism and separations will be developed, along with associated activities designed for use in the high school classroom.