Collaborative Research: Chemometrics and Comprehensive Two-Dimensional Liquid Chromatography: Toward Achieving the Promise for Metabolomics

弗吉尼亚联邦大学（VCU）的Sarah Rutan教授和明尼苏达大学的Peter Carr教授在化学系分析和表面化学项目的支持下，开发了二维液相色谱的定量方面，重点是代谢组学的应用。具体来说，本工作解决了分离条件的优化和新方法的探索，以加速二次元分离和结果数据的分析。其目的是将耗时的半定量过程转变为快速有效的方法，为生物标志物鉴定等应用提供精确的定量结果。如果成功，这项工作将为生物科学家提供重要的新工具，并将有助于提高对复杂生物系统的理解。为了使代谢组学的概念更容易被更广泛的受众所了解，将开发一个包含代谢和分离在线模拟器的网站，以及设计用于高中课堂的相关活动。

Professors Sarah Rutan at Virginia Commonwealth University (VCU) and Peter Carr at the University of Minnesota are supported by the Analytical and Surface Chemistry Program in the Division of Chemistry to develop quantitative aspects of two-dimensional liquid chromatography, with a focus on applications in metabolomics. Specifically, this work addresses the optimization of the separation conditions and exploration of novel means to accelerate both the second dimension separation and the analysis of the resulting data. The aim is to transform a time-consuming process that is semi-quantitative at best into a rapid and efficient method that will provide precise quantitative results for applications such as biomarker identification. If successful, the work will provide important new tools for biological scientists and will contribute towards improved understanding of complex biological systems. In order to make the concepts of metabolomics more accessible to a broader audience, a website with online simulators for metabolism and separations will be developed, along with associated activities designed for use in the high school classroom.