Application of the Solvatochromic Comparison Method to Chromatographic Retention and Selectivity

溶剂致变色比较法在色谱保留和选择性中的应用

• 批准号: 9521003
• 负责人: Peter Carr
• 金额: $ 25万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-12-15 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9521003&HistoricalAwards=false
• 关键词: Application; Solvatochromic; Comparison; Method; Chromatographic

This project, supported in the Analytical and Surface Chemistry Program, is directed towards the renewed study of solvation and retention mechanisms in reversed phase liquid chromatography (RPLC). During the tenure of this eighteen month standard grant, Professor Carr and his students at the University of Minnesota will expand their research in solvatochromism towards the characterization of separation processes in supercritical fluid and micellar systems. Gas chromatographic stationary phases will be designed with functional groups that participate in hydrogen bonding, acid base, and dipolar interactions with various polar and nonpolar test solutes. These interactions will be quantified using headspace gas chromatography in conjunction with linear solvation energy relations and Kamlet-Taft solvation parameters. This approach will be applied to the understanding and prediction of analyte retention in supercritical fluid chromatography and micellar electrokinetic capillary chromatography. Unlike magnetic and optical spectroscopic techniques which allow the calculation and correlation of spectral energy parameters with analyte structure, the prediction of retention profiles in reversed phase chromatography is complicated by lack of a definitive theory which can correlate molecular interactions between species in solution with the partitioning of analyte between mobile and stationary phases. Professor Carr and his students at the University of Minnesota have been leaders in development of the solvatochromic theory, which in concert with empirical chromatographic and spectroscopic measurements, attempts to quantify retention behavior in separation science. These pursuits will be expanded to the characterization of new forms of chromatography targeted towards environmental and industrial applications.

该项目得到了分析和表面化学计划的支持，旨在重新研究反相液相色谱 (RPLC) 中的溶剂化和保留机制。在这十八个月的标准拨款期间，明尼苏达大学的卡尔教授和他的学生将扩大他们在溶剂化变色方面的研究，以表征超临界流体和胶束系统中的分离过程。 气相色谱固定相将设计有参与氢键、酸碱以及与各种极性和非极性测试溶质的偶极相互作用的官能团。这些相互作用将使用顶空气相色谱法结合线性溶剂化能量关系和 Kamlet-Taft 溶剂化参数进行量化。该方法将应用于超临界流体色谱和胶束电动毛细管色谱中分析物保留的理解和预测。 与允许计算光谱能量参数并与分析物结构相关联的磁和光学光谱技术不同，反相色谱中保留曲线的预测由于缺乏可以将溶液中物质之间的分子相互作用与分析物在流动相和固定相之间的分配相关联的明确理论而变得复杂。明尼苏达大学的卡尔教授和他的学生一直是溶剂化显色理论发展的领导者，该理论与经验色谱和光谱测量相结合，试图量化分离科学中的保留行为。这些追求将扩展到针对环境和工业应用的新型色谱的表征。