Rapid Biodiversity Assessment using DNA Barcodes in Exploratories: A case study on Diptera

考虑生物多样性变化的生态研究项目需要物种清单来比较栖息地状态或分析栖息地参数实验操作的影响。传统的物种鉴定是费时的，往往需要许多分类学家的合作。DNA条形码是一个新的机会，以克服分类障碍，并产生更精确和更广泛的数据存在的物种。我们提出了一个试点研究，实施DNA条形码的物种丰富度和多样性的分析DFG生物多样性勘探站。我们专注于双翅目，是物种丰富，丰富，物种识别是困难的分类。拟议的项目有几个主要目标：开发德国双翅目的DNA条形码数据库，重点是在勘探站发现的分类群，与MOTU合作，只要Linnean名称不可用，数据库不完整，就可以对生态样品进行反向分类，为此目的开发工作流程，并测试第二代测序技术的大规模测序的可行性。该项目包括测试生态假设，如森林管理制度对摄食公会多样性的影响。

Ecological research projects that consider changes of biodiversity need inventories of species to compare habitat states or to analyze the impact of experimental manipulations of habitat parameters. Conventional species determination is time consuming and often requires the cooperation of many taxonomists. DNA barcoding is a new opportunity to overcome the taxonomic impediment and to produce more precise and extensive data on the presence of species. We propose a pilot study to implement DNA barcoding for the analysis of species richness and diversity in the DFG biodiversity exploratories. We focus on Diptera, a taxon that is species rich, abundant, and where species identification is difficult. The proposed project has several major goals: To develop a DNA barcode database for German Diptera, with a focus on taxa found in the exploratories, to work with MOTUs to apply reverse taxonomy to ecological samples as long as Linnean names are not available and the database is incomplete, to develop a workflow for this purpose, and to test the feasibility of mass-sequencing with the second-generation sequencing technology. The project includes testing of ecological hypotheses like statements about the effect of forest management regimes on the diversity of feeding guilds.