MRI: Acquisition of an Isothermal Titration Calorimeter

该奖项由2009年美国复苏和再投资法案(公法111-5)资助。通过重大研究仪器(MRI)计划的这一奖项，纽约州立大学波茨坦分校的Fadi Bou-Abdallah教授将获得一个等温滴定量热计。它将支持波茨坦大学以及附近的圣劳伦斯大学和克拉克森大学的同事的研究。主要研究将包括：1)阐明转铁蛋白如何与其特定的受体相互作用以实现铁的有效转运；2)在室温离子液体(RTIL)中酶行为的热力学表征；3)神经元特异性烯醇化酶(NSE)与抗NSE抗体之间热力学相互作用的表征；4)重金属离子与谷胱甘肽活性络合物的研究；5)调节酶Rubisco激活酶(RA)的两种异构体与Rubisco的结合化学计量比的测定以及这些结合事件的热力学表征。等温滴定量热法(ITC)是一种非常灵敏的热力学技术，用于表征物种(如小分子)与酶的结合作用。ITC直接测量在结合反应中吸收或释放的热量。这提供了对化学和生化结合相互作用强度的洞察。该仪器将用于三个合作机构的本科生的研究，并向该区域的其他机构提供。它将被并入几个高级师级别的实验室班级。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Through this award from the Major Research Instrumentation (MRI) program, Professor Fadi Bou-Abdallah at SUNY Potsdam will acquire an isothermal titration calorimeter. It will support the research of colleagues at Potsdam, and nearby St Lawrence and Clarkson Universities. The primary research studies will include, 1) elucidation of how transferrin interacts with its specific receptor to bring about the efficient delivery of iron to cells, 2) thermodynamic characterization of enzyme behavior in room temperature ionic liquids (RTILs), 3) characterization of the thermodynamic interactions between neuron specific enolase (NSE) and anti-NSE antibody, 4) studies of heavy metal ion chelates with glutathione active complexing groups, 5) determination of the binding stoichiometry of two isoforms of the regulatory enzyme Rubisco Activase (RA) with Rubisco and thermodynamic characterization of these binding events, and 6) determination of the thermodynamics of nogalamycin binding to DNA.Isothermal titration calorimetry (ITC) is a very sensitive thermodynamic technique that is used to characterize binding interactions between species such as a small molecule to an enzyme. ITC measures directly the heat absorbed or released during a binding reaction. This provides insight on the strength of chemical and biochemical binding interactions. This instrument will be used in research by undergraduates at the three collaborating institutions and made available to other institutions in the region. It will be incorporated into several upper division level laboratory classes.