MRI: Acquisition of an Isothermal Titration Calorimeter

该奖项由 2009 年美国复苏和再投资法案（公法 111-5）资助。通过主要研究仪器 (MRI) 项目的这一奖项，纽约州立大学波茨坦分校的 Fadi Bou-Abdallah 教授将获得一台等温滴定量热仪。 它将支持波茨坦以及附近圣劳伦斯大学和克拉克森大学同事的研究。 主要研究将包括：1) 阐明转铁蛋白如何与其特定受体相互作用，从而将铁有效输送至细胞；2) 室温离子液体 (RTIL) 中酶行为的热力学表征；3) 神经元特异性烯醇化酶 (NSE) 和抗 NSE 抗体之间热力学相互作用的表征；4) 重金属离子与谷胱甘肽活性络合基团的螯合物研究；5) 结合测定调节酶 Rubisco 激活酶 (RA) 的两种亚型的化学计量与 Rubisco 以及这些结合事件的热力学表征，以及 6) 确定诺加霉素与 DNA 结合的热力学。等温滴定量热法 (ITC) 是一种非常灵敏的热力学技术，用于表征物种之间的结合相互作用，例如小分子与酶的结合相互作用。 ITC 直接测量结合反应过程中吸收或释放的热量。这提供了对化学和生化结合相互作用强度的见解。该仪器将由三个合作机构的本科生用于研究，并向该地区的其他机构提供。 它将被纳入几个高年级实验室课程。

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Through this award from the Major Research Instrumentation (MRI) program, Professor Fadi Bou-Abdallah at SUNY Potsdam will acquire an isothermal titration calorimeter. It will support the research of colleagues at Potsdam, and nearby St Lawrence and Clarkson Universities. The primary research studies will include, 1) elucidation of how transferrin interacts with its specific receptor to bring about the efficient delivery of iron to cells, 2) thermodynamic characterization of enzyme behavior in room temperature ionic liquids (RTILs), 3) characterization of the thermodynamic interactions between neuron specific enolase (NSE) and anti-NSE antibody, 4) studies of heavy metal ion chelates with glutathione active complexing groups, 5) determination of the binding stoichiometry of two isoforms of the regulatory enzyme Rubisco Activase (RA) with Rubisco and thermodynamic characterization of these binding events, and 6) determination of the thermodynamics of nogalamycin binding to DNA.Isothermal titration calorimetry (ITC) is a very sensitive thermodynamic technique that is used to characterize binding interactions between species such as a small molecule to an enzyme. ITC measures directly the heat absorbed or released during a binding reaction. This provides insight on the strength of chemical and biochemical binding interactions. This instrument will be used in research by undergraduates at the three collaborating institutions and made available to other institutions in the region. It will be incorporated into several upper division level laboratory classes.