MRI: Acquisition of an Isothermal Titration Calorimeter for Research and Teaching

获得重大研究仪器(MRI)项目的这一奖项后，Brian L.McClain及其同事Eric Marinez、Vasanthy Narayamaswami、Michael Schramm和Paulus M.Weers将获得等温滴定量热计。它将用于各种研究项目，包括：1)药物结合对人血清白蛋白结构-功能关系的影响；2)α-螺旋多肽；3)受体辅助的小分子膜运输；4)阴离子和有机氨的分子识别；5)不对称Lewis酸催化的水介质中的有机反应；6)肝脏铁代谢；7)乳铁蛋白的结构-功能关系；8)载脂蛋白E和胆固醇代谢在阿尔茨海默病和心血管疾病中的作用；9)载脂蛋白在脂质运输和天然免疫中的结构作用；等温滴定量热法通过测量物质混合(滴定)时的温度变化来确定化学反应或生物分子相互作用的热力学性质，如小分子与其他物种的结合。这些变化提供了对正在发生的潜在微观过程的洞察，例如两个物种之间的结合强度或反应中释放的能量。这些仪器将用于本科生的研究和实验室课程。该仪器将设在加州州立大学长滩分校的材料、环境和社会综合研究所(IIRMES)。通过该研究所，该仪器将可以远程访问，为加州地理上分散的用户提供异地使用，特别是加州州立大学的其他校园。

With this award from the Major Research Instrumentation (MRI) program, Brian L. McClain and his colleagues Eric Marinez, Vasanthy Narayamaswami, Michael Schramm and Paulus M. Weers will acquire an isothermal titration calorimeter. It will be used in a variety of research projects including: 1) effects of drug binding on the structure-function relationships of human serum albumin; 2) alpha-helical peptidomimetics; 3) receptor assisted small molecule membrane transport; 4) molecular recognition of anions and organoammoniums; 5) asymmetric Lewis acid catalyzed organic reactions in aqueous media; 6) hepatic iron metabolism; 7) structure-function relationships of lactoferrin; 8) apolipoprotein E and cholesterol metabolism in Alzheimer's and cardiovascular diseases;9) structural role of apolipoproteins in lipid transport and innate immunity; and 10) collagen derived biomaterials.Isothermal titration calorimetry is used to determine thermodynamic properties of chemical reactions, or of biomolecular interactions such as the binding of small molecules to other species by measuring temperature changes when substances are mixed (titrated). These changes provide insight on the underlying microscopic processes that are occurring, such as the strength of the binding between the two species or the energy released in a reaction. The instruments will be used in research and laboratory classes by undergraduate students. The instrumentation will be located in the Institute for Integrated Research in Materials, Environments and Society (IIRMES) at California State University, Long Beach (CSULB). Through the Institute, the instrument will be remotely accessible, providing usability off-site to geographically dispersed users in California, particularly other CSU campuses.