MRI: Acquisition of an Isothermal Titration Calorimeter for Research and Teaching

有了这项来自主要研究仪器（MRI）项目的奖励，Brian L. McClain和他的同事Eric Marinez， Vasanthy Narayamaswami， Michael Schramm和Paulus M. Weers将获得一个等温滴定量热计。它将用于各种研究项目，包括：1)药物结合对人血清白蛋白结构-功能关系的影响；2) α -螺旋缩氨酸模拟物；3)受体辅助的小分子膜运输；4)阴离子和有机铵的分子识别；5)不对称路易斯酸在水介质中催化有机反应；6)肝铁代谢；7)乳铁蛋白的结构-功能关系；8)载脂蛋白E和胆固醇在阿尔茨海默病和心血管疾病中的代谢；载脂蛋白在脂质转运和先天免疫中的结构作用；胶原蛋白衍生的生物材料。等温滴定量热法用于确定化学反应的热力学性质，或生物分子相互作用，如小分子与其他物种的结合，通过测量物质混合（滴定）时的温度变化。这些变化提供了对正在发生的潜在微观过程的洞察，例如两种物质之间结合的强度或反应中释放的能量。这些仪器将用于本科生的研究和实验课。该仪器将位于加州州立大学长滩分校（CSULB）的材料、环境与社会综合研究所（IIRMES）。通过该研究所，该仪器可以远程访问，为加州地理位置分散的用户，特别是其他科罗拉多州立大学校园的用户提供异地可用性。

With this award from the Major Research Instrumentation (MRI) program, Brian L. McClain and his colleagues Eric Marinez, Vasanthy Narayamaswami, Michael Schramm and Paulus M. Weers will acquire an isothermal titration calorimeter. It will be used in a variety of research projects including: 1) effects of drug binding on the structure-function relationships of human serum albumin; 2) alpha-helical peptidomimetics; 3) receptor assisted small molecule membrane transport; 4) molecular recognition of anions and organoammoniums; 5) asymmetric Lewis acid catalyzed organic reactions in aqueous media; 6) hepatic iron metabolism; 7) structure-function relationships of lactoferrin; 8) apolipoprotein E and cholesterol metabolism in Alzheimer's and cardiovascular diseases;9) structural role of apolipoproteins in lipid transport and innate immunity; and 10) collagen derived biomaterials.Isothermal titration calorimetry is used to determine thermodynamic properties of chemical reactions, or of biomolecular interactions such as the binding of small molecules to other species by measuring temperature changes when substances are mixed (titrated). These changes provide insight on the underlying microscopic processes that are occurring, such as the strength of the binding between the two species or the energy released in a reaction. The instruments will be used in research and laboratory classes by undergraduate students. The instrumentation will be located in the Institute for Integrated Research in Materials, Environments and Society (IIRMES) at California State University, Long Beach (CSULB). Through the Institute, the instrument will be remotely accessible, providing usability off-site to geographically dispersed users in California, particularly other CSU campuses.