MRI: Acquisition of Instrumentation for Compound-specific Isotope Analysis at the University of Wyoming

MRI：在怀俄明大学购买用于化合物特异性同位素分析的仪器

• 批准号: 0923382
• 负责人: David Williams
• 金额: --
• 依托单位: University of Wyoming
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0923382&HistoricalAwards=false
• 关键词: MRI; Acquisition; Instrumentation; Compound; specific

Support from the National Science Foundation has allowed the purchase of a GC-combustion-isotope ratio mass spectrometer (GC-C-IRMS) and integrated quadrupole mass spectrometer (GC-MS) system with an interface supporting on-line coupling of HPLC and IRMS (LC-IRMS) to allow for stable isotope analysis (SIA). The SIA of bulk biological materials allows the integrative study of processes at scales that range from the biochemical to the biospheric. This instrumentation will add capacity for CSIA to the University of Wyoming Stable Isotope Facility (UWSIF), a core, multi-user research and training facility currently supporting 16 research groups from 8 departments and 3 colleges. The instrumentation will provide a core group of users in the capacity for CSIA on fatty acids, lipids, amino acids and carbohydrates, and be flexible enough to allow LC-IRMS on polar, non GC-volatile or thermolabile compounds without the requirement for derivatization. The major users conduct research in three areas that integrate biological and geochemical systems: 1) organismal and ecosystem ecology; 2) paleo-ecology and paleo-environmental studies; and 3) energy research. Studies with the new instruments will focus on animal physiology, diet, food webs, plant and ecosystem carbon cycling, diets and environments of extinct organisms, reconstruction of past climates and ecosystem responses, and geochemical tracing of environmental contaminants associated with energy extraction. A graduate course taught every fall semester by Williams (PI) and Sharma (co-PI) provides hands on training using the instrumentation acquired through NSF funding. High school students in the NSF EPSCoR sponsored Summer Research Apprenticeship Program (SRAP) and the NSF sponsored Science Posse program at UW have and will continue to conduct isotope-related research involving analyses in the UWSIF. These faculty also currently advise 26 graduate students (10 are women) and 4 postdocs (3 are women), and all include undergraduates in their isotope oriented research projects. Results from the research projects will be disseminated through student and faculty presentations at regional or national meetings, and through publications in peer-reviewed journals.

在国家科学基金会的支持下，购买了一台气相色谱-燃烧-同位素比质谱仪（GC-C-IRMS）和集成四极杆质谱仪（GC-MS）系统，其接口支持HPLC和IRMS（LC-IRMS）的在线耦合，以进行稳定同位素分析（SIA）。 散装生物材料的SIA允许在从生物化学到生物圈的尺度上对过程进行综合研究。 该仪器将增加怀俄明州大学稳定同位素设施（UWSIF）的CSIA能力，该设施是一个核心的多用户研究和培训设施，目前支持来自8个系和3个学院的16个研究小组。 该仪器将为脂肪酸、脂类、氨基酸和碳水化合物的CSIA提供核心用户群，并具有足够的灵活性，可以对极性、非GC挥发性或不耐热化合物进行LC-IRMS，而无需衍生化。 主要用户在整合生物和地球化学系统的三个领域进行研究：1）生物和生态系统生态学; 2）古生态学和古环境研究; 3）能源研究。使用新仪器进行的研究将侧重于动物生理学、饮食、食物网、植物和生态系统碳循环、灭绝生物的饮食和环境、过去气候和生态系统反应的重建以及与能源开采相关的环境污染物的地球化学追踪。 由威廉姆斯（PI）和夏尔马（co-PI）每年秋季学期教授的研究生课程提供使用通过NSF资助获得的仪器的实际培训。 NSF EPSCoR赞助的夏季研究学徒计划（SRAP）和NSF赞助的威斯康星大学科学波塞计划的高中生已经并将继续进行涉及UWSIF分析的同位素相关研究。 这些学院目前还为26名研究生（10名女性）和4名博士后（3名女性）提供咨询，所有这些学院都在其同位素研究项目中包括本科生。研究项目的结果将通过学生和教师在区域或国家会议上的演讲以及通过同行评审期刊上的出版物进行传播。