Acquisition of Instrumentation for Organic Molecular Geochemistry Research and Training at the University of Texas-Austin

购买德克萨斯大学奥斯汀分校有机分子地球化学研究和培训仪器

• 批准号: 0949259
• 负责人: Timothy Shanahan
• 金额: $ 9.86万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2011-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0949259&HistoricalAwards=false
• 关键词: Acquisition; Instrumentation; Organic; Molecular; Geochemistry

0949259ShanahanThis award funds acquisition of a high-performance liquid chromatography mass spectrometer (HPLC-MS) with a fraction collector to identify, quantify and isolate organic compounds for geologic matrices. The proposed acquisition will complement gas chromatographic (GC) and GC-isotope ratio mass spectrometry (GC-IRMS) instrumentation already in use. It will allow analyzing low volatility, non-GC-amenable compounds. The fraction collector will allow the PIs to purify and isolate compounds which can then be subjected to carbon isotope analysis. The instrumentation will be used for reconstructing past terrestrial, lacustrine and marine temperatures from sediment archives using ether-bound archaeal and bacterial lipids; isolating and purifying target "biomarker" compounds for stable isotope analysis using natural abundance or labeling to better understand biogeochemical cycling; and assessing organic material provenance and age in a varied depositional settings using compound-specific radiocarbon dating which will provide new constraints on the carbon cycle and the age of past sediment deposits. The underlying theme to research proposed is using compound-specific biomarkers to constrain and interpret geological data. Using HPLC-MS will provide analytical range to "new" groups of biomarkers not able to be measured by GC or other more standard techniques. Purification and isolation procedures will be developed using the fraction collector which will greatly reduce analytical labor. The instrument will be shared by several PIs and across the Department. The instrument will expand capabilities in several disciplines. Current programs are established for attracting undergraduates and disadvantaged students. Two lab-based courses will also directly benefit. The PI will retain overall supervision of the instrument. A mass spectrometry facility manager will be responsible for day-to-day scheduling. Annual maintenance and consumables costs will covered by per sample fees. Pilot studies and method(s) development will be scheduled on a case-by-case basis. The PI has two years of full-time technician support. ***

该奖项为购买一台高性能液相色谱质谱仪（HPLC-MS）提供资金，该质谱仪带有一个馏分收集器，用于鉴定、量化和分离地质基质中的有机化合物。拟议的收购将补充已经使用的气相色谱（GC）和GC-同位素比质谱（GC- irms）仪器。它将允许分析低挥发性，非气相色谱适用的化合物。馏分收集器将允许pi纯化和分离化合物，然后可以进行碳同位素分析。该仪器将用于利用醚结合古细菌和细菌脂质从沉积物档案中重建过去的陆地、湖泊和海洋温度；分离和纯化目标“生物标记”化合物，利用自然丰度或标记进行稳定同位素分析，以更好地了解生物地球化学循环；在不同的沉积环境中，使用特定化合物的放射性碳定年法来评估有机物质的来源和年龄，这将为碳循环和过去沉积物的年龄提供新的限制。提出的研究的基本主题是使用化合物特异性生物标志物来约束和解释地质数据。使用高效液相色谱-质谱将为“新的”生物标记物组提供分析范围，这些生物标记物组无法通过气相色谱或其他更标准的技术进行测量。纯化和分离程序将开发使用分馏收集器，这将大大减少分析劳动。该文书将由若干私人项目机构和整个该部共同使用。该仪器将扩展若干学科的能力。目前的项目是为了吸引本科生和弱势学生而设立的。两门实验室课程也将直接受益。PI将保留对该仪器的全面监督。质谱仪设备经理将负责日常安排。每年的维护和耗材费用将由每个样品的费用支付。试点研究和方法开发将根据具体情况安排。PI有两年的专职技术支持。＊＊＊