Identification of genes of Colletotrichum graminicola involved in establishment and maintenance of compatibility

禾谷炭疽菌参与相容性建立和维持的基因鉴定

基本信息

批准号：
19965058
负责人：
Professor Dr. Holger B. Deising
金额：
--
依托单位：
Professur für Phytopathologie und Pflanzenschutz
依托单位国家：
德国
项目类别：
Research Units
财政年份：
2006
资助国家：
德国
起止时间：
2005-12-31 至 2013-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/19965058?language=en
关键词：
Identification genes Colletotrichum graminicola involved

项目摘要

Colletotrichum graminicola is the causal agent of maize anthracnose. In a compatible interaction, this hemibiotrophic fungus sequentially establishes a biotrophic followed by a necrotrophic interaction. In order to identify genes that mediate compatibility with its host, two approaches will be taken: Agrobacterium tumefaciens-mediated transformation will allow introducing non-targeted mutations, and selected candidate compatibility genes known from other Colletotrichum species will be inactivated in C. graminicola by homologous integration of a knock-out vector. The identified compatibility genes of C. graminicola will be functionally characterized. To understand redirection of carbon flow in different phases of pathogenesis, cDNAs corresponding to fungal hexose transporter genes will be cloned by complementation of a yeast mutant deficient in 20 sugar transporter genes. Hexose transporter mutants of C. graminicola will be produced and tested for their ability to colonize their host plant and to redirect carbon flow. Mutants produced by targeted or non-targeted mutagenesis, as well as hexose transporter mutants will also be tested for their ability to induce green islands , the occurrence of which can be taken as an indication of compatibility. In a cooperative effort using Affymetrix® Gene Arrays, changes in expression profiles in maize during fungal infection will be analyzed. We will provide C. graminicola-infected tissue to allow adding data for biotrophic and necrotrophic developmental phases of a hemibiotroph.

Colletotrichum Graminicola是玉米炭疽病的因果毒剂。在兼容的相互作用中，该半野生真菌顺序建立了生物营养性，然后建立了坏死性相互作用。为了确定培养基与宿主的兼容性的基因，将采用两种方法：tumefaciens介导的农业杆菌将允许引入非靶向突变，并且通过杂物性集成在c. graminicola中被杀死的c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c. c.将法付梭菌的识别兼容基因在功能上表征。为了了解发病机理不同阶段的碳流量的重定向，通过在20种糖转运蛋白基因中酵母突变体的完成，将克隆与真菌己糖转运蛋白基因相对应的cDNA。将产生并测试法其高梭菌的己糖转运蛋白突变体，并测试其定植其宿主植物和重定向碳流量的能力。靶向或非靶向诱变产生的突变体以及己糖转运蛋白突变体也将因其诱导绿色岛屿的能力而进行测试，其发生的发生可以证明其兼容性。在使用Affymetrix®基因阵列的合作努力中，将分析真菌感染期间玉米表达谱的变化。我们将提供谷霉菌感染的组织，以允许添加半生物营养性生物营养和坏死性发育阶段的数据。