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Identification of genes of Colletotrichum graminicola involved in establishment and maintenance of compatibility

禾谷炭疽菌参与相容性建立和维持的基因鉴定

基本信息

批准号：
19965058
负责人：
Professor Dr. Holger B. Deising
金额：
--
依托单位：
Professur für Phytopathologie und Pflanzenschutz
依托单位国家：
德国
项目类别：
Research Units
财政年份：
2006
资助国家：
德国
起止时间：
2005-12-31 至 2013-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/19965058?language=en
关键词：
Identification genes Colletotrichum graminicola involved

项目摘要

Colletotrichum graminicola is the causal agent of maize anthracnose. In a compatible interaction, this hemibiotrophic fungus sequentially establishes a biotrophic followed by a necrotrophic interaction. In order to identify genes that mediate compatibility with its host, two approaches will be taken: Agrobacterium tumefaciens-mediated transformation will allow introducing non-targeted mutations, and selected candidate compatibility genes known from other Colletotrichum species will be inactivated in C. graminicola by homologous integration of a knock-out vector. The identified compatibility genes of C. graminicola will be functionally characterized. To understand redirection of carbon flow in different phases of pathogenesis, cDNAs corresponding to fungal hexose transporter genes will be cloned by complementation of a yeast mutant deficient in 20 sugar transporter genes. Hexose transporter mutants of C. graminicola will be produced and tested for their ability to colonize their host plant and to redirect carbon flow. Mutants produced by targeted or non-targeted mutagenesis, as well as hexose transporter mutants will also be tested for their ability to induce green islands , the occurrence of which can be taken as an indication of compatibility. In a cooperative effort using Affymetrix® Gene Arrays, changes in expression profiles in maize during fungal infection will be analyzed. We will provide C. graminicola-infected tissue to allow adding data for biotrophic and necrotrophic developmental phases of a hemibiotroph.

禾谷炭疽病 (Colletotrichum graminicola) 是玉米炭疽病的病原体。在相容的相互作用中，这种半生物营养真菌依次建立生物营养和死体营养相互作用。为了鉴定介导与其宿主相容性的基因，将采取两种方法：根癌农杆菌介导的转化将允许引入非靶向突变，并且通过敲除载体的同源整合，来自其他炭疽菌物种的已知候选相容性基因将在炭疽菌中失活。所鉴定的 C. graminicola 的相容性基因将进行功能表征。为了了解碳流在发病机制不同阶段的重定向，将通过补充缺乏 20 个糖转运蛋白基因的酵母突变体来克隆与真菌己糖转运蛋白基因相对应的 cDNA。将生产禾谷型 C. graminicola 的己糖转运蛋白突变体，并测试其定植宿主植物和改变碳流方向的能力。通过靶向或非靶向诱变产生的突变体以及己糖转运蛋白突变体也将测试其诱导绿岛的能力，绿岛的出现可以被视为相容性的指示。在使用 Affymetrix® 基因阵列的合作中，将分析真菌感染期间玉米表达谱的变化。我们将提供 C. graminicola 感染的组织，以便添加半生物营养体的生物营养和坏死营养发育阶段的数据。