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Identification of genes of Colletotrichum graminicola involved in establishment and maintenance of compatibility

禾谷炭疽菌参与相容性建立和维持的基因鉴定

基本信息

批准号：
19965058
负责人：
Professor Dr. Holger B. Deising
金额：
--
依托单位：
Professur für Phytopathologie und Pflanzenschutz
依托单位国家：
德国
项目类别：
Research Units
财政年份：
2006
资助国家：
德国
起止时间：
2005-12-31 至 2013-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/19965058?language=en
关键词：
Identification genes Colletotrichum graminicola involved

项目摘要

Colletotrichum graminicola is the causal agent of maize anthracnose. In a compatible interaction, this hemibiotrophic fungus sequentially establishes a biotrophic followed by a necrotrophic interaction. In order to identify genes that mediate compatibility with its host, two approaches will be taken: Agrobacterium tumefaciens-mediated transformation will allow introducing non-targeted mutations, and selected candidate compatibility genes known from other Colletotrichum species will be inactivated in C. graminicola by homologous integration of a knock-out vector. The identified compatibility genes of C. graminicola will be functionally characterized. To understand redirection of carbon flow in different phases of pathogenesis, cDNAs corresponding to fungal hexose transporter genes will be cloned by complementation of a yeast mutant deficient in 20 sugar transporter genes. Hexose transporter mutants of C. graminicola will be produced and tested for their ability to colonize their host plant and to redirect carbon flow. Mutants produced by targeted or non-targeted mutagenesis, as well as hexose transporter mutants will also be tested for their ability to induce green islands , the occurrence of which can be taken as an indication of compatibility. In a cooperative effort using Affymetrix® Gene Arrays, changes in expression profiles in maize during fungal infection will be analyzed. We will provide C. graminicola-infected tissue to allow adding data for biotrophic and necrotrophic developmental phases of a hemibiotroph.

炭疽杆菌是玉米炭疽病的病原。在相容的相互作用中，这种半生物营养真菌依次建立了一个生物营养相互作用，然后是一个坏死性相互作用。为了鉴定介导其与宿主相容性的基因，将采取两种方法：农杆菌介导的转化将允许引入非靶向突变，以及从其他炭疽菌物种中已知的候选相容性基因将通过同源整合敲除载体在C. graminicola中失活。本文将对已鉴定的禾本科真菌配型基因进行功能鉴定。为了了解碳流在不同发病阶段的重定向，将通过互补酵母中缺乏20个糖转运基因的突变体来克隆真菌己糖转运基因对应的cdna。己糖转运体突变株将被生产出来，并测试它们在寄主植物上定殖和重定向碳流的能力。通过靶向诱变或非靶向诱变产生的突变体以及己糖转运体突变体也将测试其诱导绿岛的能力，绿岛的出现可作为相容性的指示。在Affymetrix®基因阵列的合作下，将分析真菌感染期间玉米表达谱的变化。我们将提供C. graminicola感染的组织，允许添加数据的生物营养和坏死性发育阶段的半营养。