Identification of genes of Colletotrichum graminicola involved in establishment and maintenance of compatibility

禾生炭疽菌（Colletotrichum graminicola）是玉米炭疽病的病原。在相容的相互作用中，这种半活体营养型真菌依次建立活体营养型，然后是坏死营养型相互作用。为了鉴定介导与其宿主相容性的基因，将采取两种方法：根癌农杆菌介导的转化将允许引入非靶向突变，并且从其他炭疽菌物种中已知的选择的候选相容性基因将在C.通过敲除载体的同源整合将其转化到禾生菌中。鉴定出的亲和性基因。禾生菌属的功能特征。为了理解在发病机制的不同阶段中碳流的重定向，将通过20个糖转运蛋白基因缺陷的酵母突变体的互补来克隆对应于真菌己糖转运蛋白基因的cDNA。C.己糖转运蛋白突变体将生产草生菌，并测试它们在宿主植物上定居和改变碳流方向的能力。还将检测通过靶向或非靶向诱变产生的突变体以及己糖转运蛋白突变体诱导绿色岛的能力，其出现可作为相容性的指示。在一项使用Affyphase ®基因阵列的合作努力中，将分析真菌感染期间玉米中表达谱的变化。我们将提供C。禾本科菌感染的组织，以允许添加半自养生物的活体营养和坏死营养发育阶段的数据。

Colletotrichum graminicola is the causal agent of maize anthracnose. In a compatible interaction, this hemibiotrophic fungus sequentially establishes a biotrophic followed by a necrotrophic interaction. In order to identify genes that mediate compatibility with its host, two approaches will be taken: Agrobacterium tumefaciens-mediated transformation will allow introducing non-targeted mutations, and selected candidate compatibility genes known from other Colletotrichum species will be inactivated in C. graminicola by homologous integration of a knock-out vector. The identified compatibility genes of C. graminicola will be functionally characterized. To understand redirection of carbon flow in different phases of pathogenesis, cDNAs corresponding to fungal hexose transporter genes will be cloned by complementation of a yeast mutant deficient in 20 sugar transporter genes. Hexose transporter mutants of C. graminicola will be produced and tested for their ability to colonize their host plant and to redirect carbon flow. Mutants produced by targeted or non-targeted mutagenesis, as well as hexose transporter mutants will also be tested for their ability to induce green islands , the occurrence of which can be taken as an indication of compatibility. In a cooperative effort using Affymetrix® Gene Arrays, changes in expression profiles in maize during fungal infection will be analyzed. We will provide C. graminicola-infected tissue to allow adding data for biotrophic and necrotrophic developmental phases of a hemibiotroph.