Acquisition of a High-Resolution, Accurate Mass, Benchtop LC-MS with Cyber Outreach Capabilities

获取具有网络推广功能的高分辨率、精确质量、台式 LC-MS

• 批准号: 1048784
• 负责人: Dale Teeters
• 金额: $ 36.22万
• 依托单位: University of Tulsa
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-15 至 2013-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1048784&HistoricalAwards=false
• 关键词: Acquisition; Resolution; Accurate; Mass; Benchtop

With this award from the Chemistry Research Instrumentation and Facilities: Multi-user (CRIF:MU) program, Professor Dale Teeters and colleagues Tyler Johannes, Kenneth Roberts and Kerry Sublette from University of Tulsa will acquire a cyber-enabled benchtop high-resolution, accurate-mass, liquid-chromatograph mass spectrometer (LC-MS/MS). The award will enhance research training and education at all levels, especially in areas of study such as (a) determination of apurinic/apyrimidinic lesions in DNA, (b) generic engineering of algae to produce biofuels, (c) characterization of artificial i-motifs, (d) screening of proteasomal peptide products, (e) identification of intermediates in the biodegradation of organic compounds, and (f) selective carbon-carbon bond fomation methods and isolation of natural products.Mass spectrometers (MS) are used to identify the chemical composition of a sample by measuring the mass of the molecular constituents in the sample after they are ionized and detected by the mass spectrometer. Liquid chromatography is a separation technique that precedes the mass spectrometry analysis. It separates a mixture into its several constituent chemicals which are then analyzed and identified by the mass spectrometer. It is one of the fastest growing and most widely used analytical instrumentation techniques. Because of this, it is important for graduate and undergraduate students to be trained in the technique. In addition to enhancing research projects, the acquisition of an LC-MS/MS will transform several undergraduate laboratories. The cyber-enabling of the spectrometer will allow outreach to many universities in Oklahoma that have significant Native American and African American populations which are underrepresented in science and engineering.

通过化学研究仪器和设施：多用户（CRIF:MU）项目的奖励，塔尔萨大学的Dale Teeters教授和同事Tyler Johannes、Kenneth Roberts和Kerry Sublette将获得一台具有网络功能的台式高分辨率、精确质量的液相色谱质谱计（LC-MS/MS）。该奖项将加强各级的研究培训和教育，特别是在以下领域的研究：(a) DNA中无尿嘧啶/无嘧啶损伤的测定，(b)生产生物燃料的藻类通用工程，(c)人工i-motif的表征，(d)蛋白酶体肽产品的筛选，(e)有机化合物生物降解中的中间体鉴定，以及(f)选择性碳-碳键形成方法和天然产物的分离。质谱仪（MS）是通过测量样品中分子组分电离后经质谱仪检测后的质量，来鉴定样品的化学成分。液相色谱法是一种先于质谱分析的分离技术。它将混合物分离成几种化学成分，然后由质谱仪进行分析和鉴定。它是发展最快和应用最广泛的分析仪器技术之一。正因为如此，对研究生和本科生进行技术培训是很重要的。除了加强研究项目，LC-MS/MS的收购将改变几个本科实验室。光谱仪的网络启用将使俄克拉何马州的许多大学得以扩展，这些大学有大量的印第安人和非裔美国人，而这些人在科学和工程领域的代表性不足。