Acquisition of a High-Resolution, Accurate Mass, Benchtop LC-MS with Cyber Outreach Capabilities

凭借化学研究仪器和设施：多用户（CRIF：MU）计划的这一奖项，来自塔尔萨大学的Dale Teeters教授及其同事泰勒约翰内斯，肯尼斯罗伯茨和克里苏布莱特将获得一个网络支持的台式高分辨率，精确质量，液相色谱质谱仪（LC-MS/MS）。该奖项将加强各级的研究培训和教育，特别是在以下研究领域：（a）确定DNA中的脱嘌呤/脱嘧啶损伤，（B）藻类的通用工程以生产生物燃料，（c）人工i基序的表征，（d）蛋白酶体肽产物的筛选，（e）有机化合物生物降解过程中中间体的鉴定，以及（f）选择性碳-碳键形成方法和天然产物的分离。质谱仪（MS）用于通过测量样品中的分子组分在它们被电离并被质谱仪检测之后的质量来鉴定样品的化学组成。液相色谱是一种先于质谱分析的分离技术。它将混合物分离成几种化学成分，然后由质谱仪进行分析和鉴定。它是发展最快、使用最广泛的分析仪器技术之一。因此，对研究生和本科生进行技术培训非常重要。除了加强研究项目，LC-MS/MS的收购将改变几个本科实验室。光谱仪的网络使能将允许与俄克拉荷马州的许多大学进行外联，这些大学拥有大量的美洲原住民和非洲裔美国人人口，这些人口在科学和工程领域的代表性不足。

With this award from the Chemistry Research Instrumentation and Facilities: Multi-user (CRIF:MU) program, Professor Dale Teeters and colleagues Tyler Johannes, Kenneth Roberts and Kerry Sublette from University of Tulsa will acquire a cyber-enabled benchtop high-resolution, accurate-mass, liquid-chromatograph mass spectrometer (LC-MS/MS). The award will enhance research training and education at all levels, especially in areas of study such as (a) determination of apurinic/apyrimidinic lesions in DNA, (b) generic engineering of algae to produce biofuels, (c) characterization of artificial i-motifs, (d) screening of proteasomal peptide products, (e) identification of intermediates in the biodegradation of organic compounds, and (f) selective carbon-carbon bond fomation methods and isolation of natural products.Mass spectrometers (MS) are used to identify the chemical composition of a sample by measuring the mass of the molecular constituents in the sample after they are ionized and detected by the mass spectrometer. Liquid chromatography is a separation technique that precedes the mass spectrometry analysis. It separates a mixture into its several constituent chemicals which are then analyzed and identified by the mass spectrometer. It is one of the fastest growing and most widely used analytical instrumentation techniques. Because of this, it is important for graduate and undergraduate students to be trained in the technique. In addition to enhancing research projects, the acquisition of an LC-MS/MS will transform several undergraduate laboratories. The cyber-enabling of the spectrometer will allow outreach to many universities in Oklahoma that have significant Native American and African American populations which are underrepresented in science and engineering.