DNA Sequencing with Nanopores and Transverse Tunneling

利用纳米孔和横向隧道进行 DNA 测序

• 批准号: 1102230
• 负责人: Brian Willis
• 金额: $ 36万
• 依托单位: University of Connecticut
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2015-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1102230&HistoricalAwards=false
• 关键词: DNA; Sequencing; Nanopores; Transverse; Tunneling

The objective of this program is to investigate nanoelectrode nanopore DNA sequencing devices combined with a hybrid approach that measures the electrical response of the devices to short hybridized DNA sequences. The intellectual merit is to investigate a new approach to DNA sequencing that has a strong theoretical basis but needs to be developed experimentally. The proposed research will test well-developed hypotheses that DNA can be sequenced by transverse tunneling measurements. The first hypothesis is that transverse tunneling is sensitive to specific nucleotides and that sequences of nucleotides can be detected in nanoelectrode tunneling devices. The second hypothesis relaxes the need for single base resolution by using short probes that hybridize with DNA. These labeled strands are proposed to give rise to significant fluctuations of the tunneling current in nanopore devices. The broader impacts are to discover a new type of low cost DNA sequencing device that enables new medical applications for the benefit of the general public. The successful discovery of low cost nanodevices for DNA sequencing would be transformative for personalized genomics by greatly expanding our ability to study, diagnose, and treat disease. A successful outcome of the research will have a tremendous impact on healthcare world-wide by facilitating wide-spread DNA sequencing technology. The research will also foster outreach to the general public to disseminate information about the potential benefits and ethical risks of genome sequencing technology. Other broader impacts include impacts on graduate and undergraduate education and summer outreach activities to high school students, including underrepresented groups.

该计划的目的是研究纳米电极纳米孔DNA测序设备与混合方法相结合，测量设备对短杂交DNA序列的电响应。智力上的优点是研究一种新的DNA测序方法，这种方法具有强大的理论基础，但需要实验发展。这项提议的研究将测试一个成熟的假设，即DNA可以通过横向隧道测量来测序。第一个假设是，横向隧穿对特定的核苷酸敏感，并且可以在纳米电极隧穿装置中检测核苷酸序列。第二种假设通过使用与DNA杂交的短探针来减轻对单碱基分辨率的需求。这些被标记的链被提出引起纳米孔器件中隧道电流的显著波动。更广泛的影响是发现一种新型的低成本DNA测序设备，为公众带来新的医疗应用。低成本DNA测序纳米设备的成功发现将极大地扩展我们研究、诊断和治疗疾病的能力，从而为个性化基因组学带来变革。该研究的成功成果将通过促进DNA测序技术的广泛应用，对全球医疗保健产生巨大影响。这项研究还将促进与公众的接触，传播有关基因组测序技术的潜在利益和伦理风险的信息。其他更广泛的影响包括对研究生和本科教育的影响，以及对高中生（包括代表性不足的群体）的暑期拓展活动的影响。