Regulation of Arabidopsis immune function through RIN4 sub-cellular localization and exocyst interaction

通过 RIN4 亚细胞定位和外囊相互作用调节拟南芥免疫功能

• 批准号: 1120944
• 负责人: David Mackey
• 金额: $ 30万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-02-01 至 2014-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1120944&HistoricalAwards=false
• 关键词: Regulation; Arabidopsis; immune; function; through

Plants employ a branched immune system that prevents disease in the vast majority of plant-microbe interactions. One branch recognizes pathogen associated molecular patterns (PAMPs) that are contained within conserved microbial molecules. A second branch recognizes effector proteins, which are pathogen-encoded virulence factors. These two branches utilize common signaling pathways and induce common outputs, yet little is known about the mechanistic basis of intersections within the network they comprise. The RIN4 protein of the model plant Arabidopsis thaliana is a hub protein with key roles in regulating both branches, thus a detailed understanding of RIN4 will provide fundamental insight into the function of the plant immune system. The work will address three aspects of RIN4 function. Changes in the sub-cellular localization of RIN4, which are induced by bacterial effector proteins, have distinct effects on the activity of the two branches of the plant immune system. The proposed work will determine how sub-cellular localization of RIN4 affects its defense regulatory function. Preliminary data also indicate that RIN4 interacts with components of the plant exocyst, which is a multi-protein complex that regulates vesicle trafficking and membrane fusion events that play important roles in the function of the plant immune system. The proposed work will determine how interaction of the exocyst with RIN4 contributes to plant immune function. Finally, RIN4 functions in guard cells to regulate stomatal aperture in response to bacterial infection. The ability of plants to prevent invasion by epiphytically growing bacterial pathogens depends on their ability to close and keep closed their stomata. Control of guard cell function by RIN4 and bacterial effector proteins that target RIN4 will be investigated. The research aims will be addressed using plant pathology, molecular, biochemical, and cell biological methods.Broader Impacts: The trend to use agriculture to fuel as well as feed the world places great demand on optimizing crop productivity. To most effectively breed and/or engineer disease resistance in food and biofuel crops while minimizing deleterious side effects, such as reduced yield, requires fundamental knowledge about connections within the network comprising the plant immune system. Though the work will be done in the Arabidopsis thaliana model system, the findings will be applicable to agriculturally important plants, as RIN4 homologs have been identified in all land plants examined and demonstrated to play important roles in immune function of several crop plants. Findings will be presented at meetings and published in peer-reviewed journals. The PI has a proven record of working with underserved groups at the undergraduate, graduate, and post-doctoral levels, including the recent graduation of an Hispanic Ph.D. student. Funds from the project will also support participation of students from small liberal arts colleges in the Summer Undergraduate Research Experience program at The Ohio State University. Finally, the PI will participate in the organization and instruction of a module focusing on the plant immune system during the 2012 Summer Practical Workshop in Functional Genomics at Ohio State.

植物采用分支免疫系统，在绝大多数植物-微生物相互作用中预防疾病。一个分支识别保守的微生物分子中包含的病原体相关分子模式（PAMP）。第二个分支识别效应蛋白，它们是病原体编码的毒力因子。这两个分支利用共同的信号通路，并诱导共同的输出，但很少有人知道的机制基础上的交叉口内的网络，他们包括。模式植物拟南芥的RIN 4蛋白是一种枢纽蛋白，在调节两个分支中发挥关键作用，因此对RIN 4的详细了解将为了解植物免疫系统的功能提供基础知识。这项工作将解决RIN 4功能的三个方面。由细菌效应蛋白诱导的RIN 4亚细胞定位的变化对植物免疫系统的两个分支的活性具有不同的影响。这项工作将确定RIN 4的亚细胞定位如何影响其防御调节功能。初步数据还表明，RIN 4与植物外囊的组分相互作用，植物外囊是一种多蛋白复合物，调节囊泡运输和膜融合事件，这些事件在植物免疫系统的功能中发挥重要作用。拟议的工作将确定外囊与RIN 4的相互作用如何有助于植物免疫功能。最后，RIN 4在保卫细胞中起作用以调节气孔开度以响应细菌感染。植物防止附生生长的细菌病原体入侵的能力取决于它们关闭和保持关闭气孔的能力。将研究RIN 4和靶向RIN 4的细菌效应蛋白对保卫细胞功能的控制。研究目标将使用植物病理学、分子、生物化学和细胞生物学方法来解决。更广泛的影响：利用农业来为世界提供燃料和食物的趋势对优化作物生产力提出了很大的要求。为了最有效地在粮食和生物燃料作物中培育和/或工程化抗病性，同时最小化有害的副作用，例如产量降低，需要关于包括植物免疫系统的网络内的连接的基本知识。虽然这项工作将在拟南芥模型系统中完成，但这些发现将适用于农业上重要的植物，因为RIN 4同源物已在所有陆地植物中鉴定，并证明在几种作物的免疫功能中发挥重要作用。研究结果将在会议上提出，并发表在同行评审的期刊上。PI在本科生，研究生和博士后水平上与服务不足的群体合作的记录已经得到证实，包括最近毕业的西班牙裔博士。学生.该项目的资金还将支持来自小型文理学院的学生参加俄亥俄州州立大学的夏季本科生研究体验计划。最后，PI将在俄亥俄州举办的2012年夏季功能基因组学实践研讨会期间参与组织和指导一个侧重于植物免疫系统的模块。