Regulation of Arabidopsis Defense Signaling By RIN4 and Associated Proteins

RIN4 和相关蛋白对拟南芥防御信号的调节

• 批准号: 0718882
• 负责人: David Mackey
• 金额: $ 51.07万
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-08-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0718882&HistoricalAwards=false
• 关键词: Regulation; Arabidopsis; Defense; Signaling; RIN4

Plants have a sophisticated, two-branched innate immune system that induces resistance against nearly all would-be pathogens. The first branch is basal defense, which is induced in response to widely conserved pathogen-associated patterns. The second branch is plant cell-death associated resistance, which is induced by interactions between genes that are variably expressed in both plants and pathogens. Although these branches had long been considered distinct, studies on the RIN4 protein from the model plant, Arabidopsis thaliana, have demonstrated a mechanistic link. Specifically, RIN4 is a regulator of both branches of the plant immune system. This project includes study of how RIN4 modification by phosphorylation regulates its function and how RIN4-interacting proteins mediate activity of RIN4. These studies will significantly advance understanding of how each branch functions in Arabidopsis. More importantly, the relationship between the two branches will be elucidated, which will advance understanding of the evolution and function of the immune systems of all plants. By understanding how these two branches work together to resist nearly all infections, strategies for disease control can be developed in those cases where the plant immune system fails. Furthermore, because similar linkages are just being discovered to exist between branches of the innate immune system of animals, this work in plants will continue to lead advancement across the field of immunology.The results of this work will be presented at meetings and published in peer-reviewed journals. The implications of this work to agriculture are obvious. The PI has a proven record of working with under-represented minorities, including undergraduate students, doctoral students, and post-docs. Also, the investigator is a leader in establishing the summer undergraduate research experience (SURE) program to provide internships for undergraduates considering careers in science.

植物有一个复杂的、两个分支的天然免疫系统，可以诱导对几乎所有可能的病原体产生抵抗力。第一个分支是基础防御，它是对广泛保守的病原体相关模式的反应而诱导的。第二个分支是植物细胞死亡相关抗性，它是由在植物和病原菌中可变表达的基因之间的相互作用诱导的。尽管这些分支长期以来一直被认为是不同的，但对模式植物拟南芥的RIN4蛋白的研究已经证明了一种机械联系。具体地说，RIN4是植物免疫系统的两个分支的调节器。该项目包括研究RIN4如何通过磷酸化修饰来调节其功能，以及RIN4相互作用的蛋白质如何介导RIN4的活性。这些研究将极大地促进对每个分支如何在拟南芥中发挥作用的了解。更重要的是，这两个分支之间的关系将被阐明，这将促进对所有植物免疫系统的进化和功能的了解。通过了解这两个分支是如何共同抵御几乎所有感染的，就可以在植物免疫系统失效的情况下制定疾病控制策略。此外，由于刚刚发现动物先天免疫系统的分支之间存在类似的联系，这项在植物上的工作将继续引领整个免疫学领域的进步。这项工作的结果将在会议上公布，并发表在同行评议的期刊上。这项工作对农业的影响是显而易见的。国际和平协会有与代表不足的少数群体合作的可靠记录，包括本科生、博士生和博士后。此外，这位调查员是建立暑期本科生研究体验(SURE)计划的领导者，该计划为考虑从事科学职业的本科生提供实习机会。