CPS:Medium:Hardware/Software Co-Design for the Life Sciences: Towards a Programmable and Reconfigurable Lab-on-Chip

CPS：中：生命科学的硬件/软件协同设计：迈向可编程和可重新配置的片上实验室

• 批准号: 1135853
• 负责人: Krishnendu Chakrabarty
• 金额: $ 160万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2017-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1135853&HistoricalAwards=false
• 关键词: CPS; Medium; Hardware; Software; Co

This project integrates digital microfluidics with thin-film photodetectors and control software to realize DNA target sensing using fluorescence. This cyberphysical vision is being realized through tight coupling between physical components?the microfluidic platform and miniaturized sensors, and cyber components?software for control, decision-making, and adaptation. Such a level of integration, decision, and controlled reconfigurability is a significant step forward in clinical diagnostics using digital microfluidic biochips. Topics being investigated include: (i) silicon-based digital microfluidics and integration of optical sensors; (ii) closed-loop operation and run-time optimization under software control; (iii) decision-tree architectures, adaptive reconfiguration, and error recovery. A complete testbed is being developed for nucleic acid identification on a fabricated chip with detection sites.Cyberphysical system integration will transform biochip use, in the same way as compilers and operating systems revolutionized computing, and design automation revolutionized chip design. Benefits to society include the potential to transform personalized medicine, home diagnostics, and portable diagnostics. Integration of digital microfluidics, optical sensing, and software control has the potential to create systems that can be used by any person, regardless of sample preparation skill. One example is the identification of bacterial DNA associated with bacterial blood infection (sepsis), which results in death if not diagnosed early (this is in the top 10 causes of death in the US). Students are being trained through a Senior Design course to understand the design

本项目将数字微流控技术与薄膜光电探测器和控制软件相结合，实现了DNA靶标的荧光传感。通过物理组件-微流控平台和微型传感器-与网络组件-用于控制、决策和适应的软件-之间的紧密耦合，实现了这一网络物理愿景。这种集成、决策和受控可重构性的水平是使用数字微流控生物芯片进行临床诊断的重要一步。正在研究的主题包括：(I)硅基数字微流体和光学传感器的集成；(Ii)软件控制下的闭环操作和运行时间优化；(Iii)决策树体系结构、自适应重新配置和错误恢复。一种完整的试验台正在开发中，用于在带有检测站点的制造芯片上进行核酸识别。网络物理系统集成将改变生物芯片的使用，就像编译器和操作系统革命性地计算一样，设计自动化将革命性地改变芯片设计。对社会的好处包括改变个性化医疗、家庭诊断和便携式诊断的潜力。数字微流控、光学传感和软件控制的集成有可能创造出任何人都可以使用的系统，无论样品制备技术如何。一个例子是鉴定与细菌血液感染(败血症)有关的细菌DNA，如果不及早诊断，就会导致死亡(这是美国前十大死因之一)。学生正在接受高级设计课程的培训，以理解设计