MRI: Acquisition of a Confocal Microscope for Multidisciplinary Research

MRI：购买共焦显微镜进行多学科研究

• 批准号: 1229237
• 负责人: Marcus Davis
• 金额: $ 33.46万
• 依托单位: Kennesaw State University Research and Service Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1229237&HistoricalAwards=false
• 关键词: MRI; Acquisition; Confocal; Microscope; Multidisciplinary

The Zeiss LSM 700 Laser Scanning Confocal Imaging System will be used in multidisciplinary research and education in the Departments of Biology & Physics and Chemistry and Biochemistry at Kennesaw State University (KSU) and will be the anchor instrument of KSU's microscopy core facility in the new Science Laboratory Building. The LSM 700 and integrated workstation with ZEN (Zeiss Efficient Navigation) software is capable of covering a broad range of imaging needs, from multiple fluorescence and co-localization analysis to live cell imaging to photoactivation of fluorophores. This flexibility will maximize the number of researchers, and types of research, that can make use of the microscope. The confocal imaging system will advance KSU research programs, foster new research directions, and generate data that will complement many other techniques employed by researchers at KSU, such as: fluorescent and standard microscopy, cell culture and in vivo assays, gel-shift, equilibrium saturation and competition assays. The LSM 700 system will enhance the ability of KSU researchers to generate and interpret data. The integration of confocal results with experiments already performed at KSU will provide crucial insights for on-going research in developmental biology, molecular genetics, neurobiology, botany, ecology, evolutionary biology, biochemistry, and related fields at KSU and our collaborator institutions. Although the primary use of the microscope will be for research, it will also be integrated into research training and course curricula. Confocal microscopy is an increasingly common research tool for visualizing biological and biochemical systems. Acquisition of the LSM 700 confocal microscope will have a significant impact on the research environment at Kennesaw State University (KSU), giving researchers in the Departments of Biology & Physics and Chemistry & Biochemistry the ability to more fully describe interactions important to the systems they study. Publication of innovative research by KSU faculty incorporating confocal data will provide insights into such fields as cell and developmental biology, biochemistry, ecology, evolutionary biology, and anatomy. In addition, the instrument broadens the array of research tools available to researchers at KSU and makes them more attractive collaborators for scientists at more research-intensive institutions. Beyond primary research, teaching experiments will be designed to incorporate confocal microscopy into the classroom. Bringing a more quantitative approach to bear on research problems using confocal microscopy will also make faculty better teachers and afford invaluable research training to undergraduates, including members of underrepresented groups. Hands-on training in the technology and usage of confocal imaging will better prepare KSU graduates for STEM careers.

蔡司LSM 700激光扫描共聚焦成像系统将用于肯尼索州立大学（KSU）生物物理系、化学系和生物化学系的多学科研究和教育，并将成为新科学实验楼中KSU显微镜核心设施的锚仪器。 LSM 700和集成ZEN（Zeiss Efficient Navigation）软件的工作站能够满足广泛的成像需求，从多重荧光和共定位分析到活细胞成像，再到荧光团的光活化。这种灵活性将最大限度地增加研究人员的数量，以及可以使用显微镜的研究类型。共聚焦成像系统将推进KSU的研究计划，培养新的研究方向，并生成数据，以补充KSU研究人员采用的许多其他技术，例如：荧光和标准显微镜，细胞培养和体内测定，凝胶位移，平衡饱和和竞争测定。LSM 700系统将提高KSU研究人员生成和解释数据的能力。 共聚焦结果与已经在KSU进行的实验的整合将为KSU和我们的合作机构正在进行的发育生物学，分子遗传学，神经生物学，植物学，生态学，进化生物学，生物化学和相关领域的研究提供重要的见解。虽然显微镜的主要用途将是研究，但它也将被纳入研究培训和课程。共聚焦显微镜是一种越来越常见的研究工具，用于可视化生物和生化系统。 LSM 700共聚焦显微镜的收购将对肯尼索州立大学（KSU）的研究环境产生重大影响，使生物物理和化学生物化学系的研究人员能够更全面地描述对他们研究的系统至关重要的相互作用。 KSU教师将共聚焦数据纳入创新研究的出版物将提供对细胞和发育生物学，生物化学，生态学，进化生物学和解剖学等领域的见解。此外，该仪器扩大了KSU研究人员可用的研究工具的范围，使他们成为研究密集型机构的科学家更具吸引力的合作者。 除了主要的研究，教学实验将被设计为将共聚焦显微镜纳入课堂。带来一个更定量的方法来承担使用共聚焦显微镜的研究问题，也将使教师更好的教师，并提供宝贵的研究培训的本科生，包括代表性不足的群体的成员。 在共焦成像的技术和使用的动手培训将更好地准备KSU毕业生干的职业生涯。

项目成果

Fin-fold development in paddlefish and catshark and implications for the evolution of the autopod

• DOI: 10.1098/rspb.2016.2780
• 发表时间: 2017-05
• 期刊: Proceedings of the Royal Society B: Biological Sciences
• 作者: Frank J. Tulenko;James L. Massey;E. Holmquist;Gabriel Kigundu;Sarah Thomas;Susan M E Smith;S. Mazan;Marcus C. Davis