MAS NMR-based studies of phytochrome intramolecular function and signalling

基于 MAS NMR 的光敏色素分子内功能和信号传导研究

• 批准号: 207453734
• 负责人: Professor Dr. Jonathan Hughes, Ph.D.
• 金额: --
• 依托单位: Institut für Analytische Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/207453734?language=en
• 关键词: MAS; NMR; based; studies; phytochrome

Phytochromes are photoreceptor molecules widely used in nature to perceive the light environment - for example inducing germination, flowering etc. in plants. We have successfully applied solid-state NMR methods to the cyanobacterial phytochrome Cph1 to find out how phytochromes work. We propose to study how photons de/active the molecule from the primary photoreaction through the protein, eventually to regulate its biochemical activity in the cell. Amino acid types and/or the chromophore will be 13C/15N-labelled pairwise so that interactions and light-dependent changes can be studied throughout the protein. To this end we will also develop methods to allow interatomic distances to be measured accurately. Using in situ irradiation and freeze-trapping we will also study intermediates, deepening our understanding of the underlying the photoreaction per se and the ensuing intramolecular signal transduction mechanism. We will also extend our studies to include specific mutants, allowing us to investigate the mechanism experimentally. We will also study other phytochromes, including those from plants themselves. The information gained will be relevant to the phytochrome photoreceptor family but also to related regulatory molecules, for example the sensory kinases used by microorganisms including pathogens to perceive and respond to their environment.

光敏色素是自然界中广泛使用的感光分子，用于感知光环境，例如诱导植物萌发、开花等。我们已经成功地将固体核磁共振方法应用于蓝藻光敏色素Cph1，以了解光敏色素的工作原理。我们建议研究光子如何通过蛋白质从初级光反应中去除/激活分子，最终调节其在细胞中的生化活性。氨基酸类型和/或发色团将被13C/15N成对标记，这样就可以在整个蛋白质中研究相互作用和与光相关的变化。为此，我们还将开发能够准确测量原子间距离的方法。利用原位辐射和冷冻捕获，我们还将研究中间体，加深我们对潜在的光反应本身和随后的分子内信号转导机制的理解。我们还将扩大我们的研究范围，包括特定的突变体，使我们能够通过实验研究这一机制。我们还将研究其他光敏色素，包括那些来自植物本身的光敏色素。所获得的信息将与光敏色素光受体家族相关，但也与相关的调节分子有关，例如微生物(包括病原体)用来感知和响应环境的感觉蛋白激酶。

项目成果

Structural insights into photoactivation and signalling in plant phytochromes

• DOI: 10.1038/s41477-020-0638-y
• 发表时间: 2020-05-04
• 期刊: NATURE PLANTS
• 影响因子: 18
• 作者: Nagano, Soshichiro;Guan, Kaoling;Hughes, Jon
• 通讯作者: Hughes, Jon

Dynamic Nuclear Polarization Provides New Insights into Chromophore Structure in Phytochrome Photoreceptors.

• DOI: 10.1002/anie.201608119
• 发表时间: 2016-11
• 期刊: Angewandte Chemie
• 作者: Daniel Stöppler;Chen Song;B. van Rossum;Michel-Andreas Geiger;C. Lang;M. Mroginski;A. P. Jagtap;S. Sigurdsson;J. Matysik;J. Hughes;H. Oschkinat

Crystal Effects on Mesobilirubin: A Combined NMR Spectroscopic and Density Functional Theory Study

晶体对中胆红素的影响：核磁共振波谱和密度泛函理论相结合的研究

• DOI: 10.1111/php.12734
• 发表时间: 2017
• 期刊: Photochemistry and Photobiology
• 影响因子: 3.3
• 作者: Chen Song;Jörg Matysik;Franz Mark
• 通讯作者: Franz Mark

Structural communication between the chromophore‐binding pocket and the N‐terminal extension in plant phytochrome phyB

植物光敏色素 phyB 中发色团结合袋和 N 末端延伸之间的结构通讯

• DOI: 10.1002/1873-3468.12642
• 发表时间: 2017
• 期刊: FEBS Letters
• 影响因子: 3.5
• 作者: Francisco Velázquez Escobar;David Buhrke;Maria Fernandez Lopez;Sintayehu Shenkutie;Sylvia von Horsten;Lars-Oliver Essen;Jon Hughes;Peter Hildebrandt
• 通讯作者: Peter Hildebrandt

MAS NMR on a Red/Far-Red Photochromic Cyanobacteriochrome All2699 from Nostoc

来自发菜的红色/远红光致变色蓝藻色素 All2699 的 MAS NMR

• DOI: 10.3390/ijms20153656
• 发表时间: 2019-07
• 期刊: International Journal of Molecular Sciences
• 影响因子: 5.6
• 作者: Xu Qian-Zhao;Bielytskyi Pavlo;Otis James;Lang Christina;Hughes Jon;Zhao Kai-Hong;Losi Aba;Gaertner Wolfgang;Song Chen
• 通讯作者: Song Chen