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Phytochrome cytoplasmic signalling in lower plants

低等植物光敏色素细胞质信号传导

基本信息

批准号：
22237773
负责人：
Professor Dr. Jonathan Hughes, Ph.D.
金额：
--
依托单位：
Biochemisches Institut
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2006
资助国家：
德国
起止时间：
2005-12-31 至 2018-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/22237773?language=en
关键词：
Phytochrome cytoplasmic signalling lower plants

项目摘要

Phytochrome photoreceptors play a central role in light-regulated development in plants. In their Pr ground state plant phytochromes are generally cytosolic but red light absorption induces the Pfr signalling state, a proportion of which is then transported into the nucleus to regulate transcription. However, some phytochrome effects are too fast for transcriptional changes to be involved. Also phytochrome in lower plants provides vectorial information for directional growth of individual cells and chloroplast relocation: this cannot be based on transcription regulation. Thus phytochrome must have another signalling mechanism within the cytoplasm. We have now identified and initially characterized this as arising from a hitherto unexpected interaction of a sub-population of phytochrome molecules with phototropin at the plasma membrane in Physcomitrella (Jaedicke et al. (2012) PNAS). The purpose of this project is to describe the complex and its signaling mechanism at the molecular level. To this end we will exploit the Physcomitrella model system physiologically (photo- and polarotropism) and genetically (genome sequence and homologous recombination) with the help of molecular genetic, cell biological, biochemical and biophysical methods. We propose to continue our work using a variety of yeast two-hybrid approaches to identify potential cytoplasmic phytochrome interactors and to characterize the associated signaling system by bimolecular fluorescence complementation, fluorescence lifetime imaging, co-immunoprecipitation, targetted knockout / complementation and other techniques. Although we have evidence that the phy-phot interaction occurs in Arabidopsis too, we do not propose to study this in the present application.

光敏色素光感受器在植物的光调控发育中起着重要作用。在它们的Pr基态植物光敏色素通常是胞质的，但是红光吸收诱导Pfr信号传导状态，其中一部分然后被运输到细胞核中以调节转录。然而，一些光敏色素效应太快，转录变化无法参与。低等植物中的光敏色素也为单个细胞的定向生长和叶绿体的重新定位提供了矢量信息：这不能基于转录调控。因此光敏色素在细胞质内一定有另一种信号机制。我们现在已经鉴定并初步表征了这是由光敏色素分子的亚群与立碗藓属质膜处的光促素的迄今为止意想不到的相互作用引起的（Jaedicke等人（2012）PNAS）。本项目的目的是在分子水平上描述该复合物及其信号传导机制。为此，我们将利用物理小立碗藓模型系统生理（光和极性）和遗传（基因组序列和同源重组）的帮助下，分子遗传学，细胞生物学，生物化学和生物物理学方法。我们建议继续我们的工作，使用各种酵母双杂交方法来确定潜在的细胞质光敏色素相互作用，并通过双分子荧光互补，荧光寿命成像，免疫共沉淀，靶向敲除/互补和其他技术来表征相关的信号系统。虽然我们有证据表明phy-phot相互作用也发生在拟南芥中，但我们不打算在本申请中研究这一点。