Elektrospray-Quadrupol-Time-of-Flight-Massenspektrometer

电喷雾四极杆飞行时间质谱仪

• 批准号: 208680633
• 金额: --
• 依托单位: Universität zu Köln
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/208680633?language=en
• 关键词: Elektrospray; Quadrupol; Time; Flight; Massenspektrometer

Mass spectrometry has become extremely important in biological and life sciences. Like in other universities, MS equipment and specific methods mainly for proteomics and for lipid analytics have been or are currently being established within the University of Cologne at the Center for Molecular Medicine Cologne (CMMC), within the CECAD, and the new MS platform in the Biocenter of the Department of Biology (BIO-MS platform). After the move of the research group of Prof. Dr. Dietmar Schomburg from Cologne to the Technical University of Braunschweig in 2007, experimental/bioinformatics research on metabolic networks and enzymatic function using metabolome analysis/mass spectrometry became largely inexistent in the biological sciences in Cologne. This gap should now at least in part be filled with the technological and methodological portfolio of the BIO-MS platform through establishment of metabolome analysis using an ESI-Q-TOF MS. Moreover, ultrahigh resolution and the possible online coupling to a HPLC or GC system allows for the detection of soluble or gaseous metabolites. This makes this instrument a high-end, state-of-the-art equipment for metabolomics research for the next ten to fifteen years.

质谱分析在生物和生命科学中变得极其重要。与其他大学一样，主要用于蛋白质组学和脂质分析的MS设备和特定方法已经或目前正在科隆大学分子医学中心（CMMC），CECAD和生物系生物中心的新MS平台（BIO-MS平台）内建立。Dietmar Schomburg教授博士的研究小组于2007年从科隆搬到布伦瑞克技术大学后，在科隆的生物科学中，使用代谢组分析/质谱法对代谢网络和酶功能进行实验/生物信息学研究在很大程度上变得不存在。这一差距现在应该至少部分地填补了BIO-MS平台的技术和方法组合，通过使用ESI-Q-TOF MS建立代谢组学分析。此外，HPLC或GC系统的快速分离和可能的在线耦合允许检测可溶性或气态代谢物。这使得该仪器成为未来十到十五年代谢组学研究的高端，最先进的设备。

项目成果

MYB-FL controls gain and loss of floral UV absorbance, a key trait affecting pollinator preference and reproductive isolation

• DOI: 10.1038/ng.3462
• 发表时间: 2016-02-01
• 期刊: NATURE GENETICS
• 影响因子: 30.8
• 作者: Sheehan, Hester;Moser, Michel;Kuhlemeier, Cris
• 通讯作者: Kuhlemeier, Cris