Single-Molecule Methods for Interfacial Dynamics

界面动力学的单分子方法

基本信息

  • 批准号:
    1306108
  • 负责人:
  • 金额:
    $ 49.3万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2013
  • 资助国家:
    美国
  • 起止时间:
    2013-09-15 至 2016-08-31
  • 项目状态:
    已结题

项目摘要

In this research project funded by the Chemical Measurements and Imaging program in the Chemistry Division, Prof. Daniel K Schwartz and his group at the University of Colorado at Boulder will develop single-molecule (SM) imaging methods, based on total internal reflection fluorescence (TIRF) microscopy, including dynamic SM resonance energy transfer (RET) methods, to probe the nm-scale molecular environment and molecular conformation, while simultaneously observing dynamic molecular behavior at the solid/liquid interface, including adsorption, desorption, intermolecular association (i.e., aggregation), and interfacial diffusion. Specific experiments are being performed that will (1) link molecular conformation directly to interfacial dynamics, and (2) explicitly probe the dynamics of intermolecular association (e.g. clustering, aggregation) in the near-surface environment. Some of the new methods involve intermolecular RET between mobile donor-labeled probes and an acceptor-labeled molecular matrix. These methods provide direct information about the ways in which the interfacial environment affects adsorption, surface residence time, interfacial mobility, and molecular recognition kinetics. Other methods use intramolecular RET to probe conformational changes of polypeptides and oligonucleotides, and to correlate these conformational changes with dynamic interfacial behavior. The methods developed as part of this research represent enabling new techniques that will be broadly used in the research community to study materials interfaces, especially "wet" interfaces of soft materials such as polymers. An improved understanding of these phenomena will lead to improvements in biomaterials, pharmaceutical separations and formulation, medical diagnostics based on molecular recognition, and self-assembled nanomaterials. Interactions between surfaces and biomolecules have widespread relevance to biotechnology and human health, affecting medical diagnostics, therapeutic protein stability, vaccine efficacy/safety, and biomaterial design. In particular, the methods developed here will be widely-applicable to biomaterials characterization, permitting a new level of understanding of the ways in which surface chemistry influences molecular conformation and lateral interactions. The researchers involved in this project will gain expertise in multidisciplinary areas projected to be at the forefront of science and technology in the coming decades, including single-molecule microscopy and self-assembled molecular systems. Also, through their participation in a variety of educational and outreach programs, the researchers will share this project with students, teachers, and other community members.
在这个由化学部化学测量和成像项目资助的研究项目中,科罗拉多大学博尔德分校的丹尼尔K施瓦茨教授及其团队将开发基于全内反射荧光(TIRF)显微镜的单分子(SM)成像方法,包括动态SM共振能量转移(RET)方法,以探测纳米级分子环境和分子构象,同时观察固/液界面处的动态分子行为,包括吸附、解吸,分子间缔合(即,聚集)和界面扩散。正在进行的具体实验将(1)将分子构象直接与界面动力学联系起来,以及(2)明确探测近表面环境中分子间缔合的动力学(例如聚类、聚集)。 一些新方法涉及移动的供体标记探针和受体标记分子基质之间的分子间RET。这些方法提供了界面环境影响吸附、表面停留时间、界面流动性和分子识别动力学的直接信息。其他方法使用分子内RET来探测多肽和寡核苷酸的构象变化,并将这些构象变化与动态界面行为相关联。作为本研究的一部分开发的方法代表了将在研究界广泛使用的新技术,以研究材料界面,特别是软材料(如聚合物)的“湿”界面。对这些现象的更好理解将导致生物材料、药物分离和制剂、基于分子识别的医学诊断和自组装纳米材料的改进。表面和生物分子之间的相互作用与生物技术和人类健康具有广泛的相关性,影响医学诊断、治疗性蛋白质稳定性、疫苗功效/安全性和生物材料设计。特别是,这里开发的方法将广泛适用于生物材料的表征,允许一个新的水平的理解,其中表面化学影响分子构象和横向相互作用的方式。参与该项目的研究人员将获得多学科领域的专业知识,这些领域预计将在未来几十年内成为科学和技术的前沿,包括单分子显微镜和自组装分子系统。 此外,通过参与各种教育和推广计划,研究人员将与学生,教师和其他社区成员分享这个项目。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Daniel Schwartz其他文献

Parallel Assertions for Architectures with Weak Memory Models
具有弱内存模型的架构的并行断言
A solver-aided language for test input generation
用于生成测试输入的求解器辅助语言
VENOUS COLLAGENOSIS, WHITE MATTER HYPERINTENSITY AND THE PERIVASCULAR SPACE
  • DOI:
    10.1016/j.cccb.2022.100098
  • 发表时间:
    2024-01-01
  • 期刊:
  • 影响因子:
  • 作者:
    David Lahna;Daniel Schwartz;Randy Woltjer;Sandra Black;Natalie Roese;Hiroko Dodge;Erin Boespflug;Julia Keith;Fuqiang Gao;Joel Ramirez;Lisa Silbert
  • 通讯作者:
    Lisa Silbert
Paramedics successfully perform humeral EZ-IO intraosseous access in adult out-of-hospital cardiac arrest patients
  • DOI:
    10.1016/j.ajem.2011.07.010
  • 发表时间:
    2012-09-01
  • 期刊:
  • 影响因子:
  • 作者:
    David Wampler;Daniel Schwartz;Joi Shumaker;Scotty Bolleter;Robert Beckett;Craig Manifold
  • 通讯作者:
    Craig Manifold
Risk factors for developing multiple sclerosis after childhood optic neuritis: Lucchinetti CF, Kiers L, O'Duffy A, Gomez MR, Cross S, Leavitt JA, O'Brien P, Rodriguez M<sup>*</sup>. Neurology 1997;49:1413–1418
  • DOI:
    10.1016/s0002-9394(99)80179-2
  • 发表时间:
    1998-03-01
  • 期刊:
  • 影响因子:
  • 作者:
    Bruce E. Silverstein;J. Hasenyager Smith;Scott O. Sykes;Matthew R. Jones;Daniel Schwartz;Emmett T. Cunningham
  • 通讯作者:
    Emmett T. Cunningham

Daniel Schwartz的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Daniel Schwartz', 18)}}的其他基金

Catalyzing Electrochemical Data Sciences: Education and Research Opportunities Workshop
催化电化学数据科学:教育和研究机会研讨会
  • 批准号:
    2034796
  • 财政年份:
    2020
  • 资助金额:
    $ 49.3万
  • 项目类别:
    Standard Grant
Measuring the benefits of museum experiences as preparation for future learning
衡量博物馆体验的好处,为未来的学习做好准备
  • 批准号:
    1337414
  • 财政年份:
    2013
  • 资助金额:
    $ 49.3万
  • 项目类别:
    Standard Grant
Nimble Assessments: Tools for the Design and Analysis of Interactive Assessments
Nimble 评估:交互式评估设计和分析的工具
  • 批准号:
    1228831
  • 财政年份:
    2012
  • 资助金额:
    $ 49.3万
  • 项目类别:
    Standard Grant
Methods for Detecting Nucleic Acid Hybridization Using Liquid Crystals
使用液晶检测核酸杂交的方法
  • 批准号:
    1160202
  • 财政年份:
    2012
  • 资助金额:
    $ 49.3万
  • 项目类别:
    Standard Grant
I-Corps: Commercialization of an Engineered Pyrolysis Blanket for the Economic Conversion of Forestry Residues to Soil Amendments and Energy Products
I-Corps:工程热解毯的商业化,用于将林业残留物经济转化为土壤改良剂和能源产品
  • 批准号:
    1158809
  • 财政年份:
    2011
  • 资助金额:
    $ 49.3万
  • 项目类别:
    Standard Grant
HCC: Medium: Collaborative Research: Formal Analysis of Choice-Adaptive Intelligent Learning Environments (FACILE) that support Future Learning
HCC:媒介:协作研究:支持未来学习的选择自适应智能学习环境 (FACILE) 的形式分析
  • 批准号:
    0904324
  • 财政年份:
    2009
  • 资助金额:
    $ 49.3万
  • 项目类别:
    Standard Grant
Single-Molecule Studies of Surfactant Mobility at the Solid/Solution Interface
固体/溶液界面表面活性剂迁移率的单分子研究
  • 批准号:
    0841116
  • 财政年份:
    2009
  • 资助金额:
    $ 49.3万
  • 项目类别:
    Standard Grant
Collaborative Research: Line-Active Amphiphiles for Nanostructure Stability
合作研究:用于纳米结构稳定性的线活性两亲物
  • 批准号:
    0906735
  • 财政年份:
    2009
  • 资助金额:
    $ 49.3万
  • 项目类别:
    Continuing Grant
REU Site Program in Functional Materials
REU 功能材料现场计划
  • 批准号:
    0851849
  • 财政年份:
    2009
  • 资助金额:
    $ 49.3万
  • 项目类别:
    Standard Grant
Cognitive and cortical restructuring in the acquisition of negative number concepts.
负数概念习得中的认知和皮质重组。
  • 批准号:
    0814768
  • 财政年份:
    2008
  • 资助金额:
    $ 49.3万
  • 项目类别:
    Continuing Grant

相似国自然基金

D-A类共轭聚合物晶界内部tie molecule构象调控
  • 批准号:
    51573185
  • 批准年份:
    2015
  • 资助金额:
    70.0 万元
  • 项目类别:
    面上项目
耦合可积系统及其molecule解的研究
  • 批准号:
    11026119
  • 批准年份:
    2010
  • 资助金额:
    3.0 万元
  • 项目类别:
    数学天元基金项目

相似海外基金

Photo-Fragmentation Methods for Single-Molecule Protein Sequencing by Nanopore Mass Spectrometry
纳米孔质谱单分子蛋白质测序的光断裂方法
  • 批准号:
    10644378
  • 财政年份:
    2023
  • 资助金额:
    $ 49.3万
  • 项目类别:
MCA: Understanding cellulose synthase complex in planta using single molecule methods
MCA:使用单分子方法了解植物中的纤维素合酶复合物
  • 批准号:
    2321398
  • 财政年份:
    2023
  • 资助金额:
    $ 49.3万
  • 项目类别:
    Standard Grant
Development of integrative methods for correlative studies among dynamics, nano-localization, and function using simultaneous multi-color single-molecule and super-resolution imaging
使用同时多色单分子和超分辨率成像开发动力学、纳米定位和功能之间相关研究的综合方法
  • 批准号:
    22H02581
  • 财政年份:
    2022
  • 资助金额:
    $ 49.3万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Developing super-resolution imaging based biophysical methods to study protein aggregate induced membrane disruption at the single-molecule level
开发基于超分辨率成像的生物物理方法来研究单分子水平上蛋白质聚集诱导的膜破坏
  • 批准号:
    2594676
  • 财政年份:
    2021
  • 资助金额:
    $ 49.3万
  • 项目类别:
    Studentship
Extending the temporal and spatial capabilities of single-molecule methods
扩展单分子方法的时间和空间能力
  • 批准号:
    10478197
  • 财政年份:
    2021
  • 资助金额:
    $ 49.3万
  • 项目类别:
Extending the temporal and spatial capabilities of single-molecule methods
扩展单分子方法的时间和空间能力
  • 批准号:
    10281044
  • 财政年份:
    2021
  • 资助金额:
    $ 49.3万
  • 项目类别:
RUI: Characterizing DNA target search using single molecule methods
RUI:使用单分子方法表征 DNA 靶标搜索
  • 批准号:
    2120878
  • 财政年份:
    2021
  • 资助金额:
    $ 49.3万
  • 项目类别:
    Standard Grant
Development of advanced single-molecule fluorescence imaging methods to visualize coupled conformational changes in transcription
开发先进的单分子荧光成像方法,以可视化转录中的耦合构象变化
  • 批准号:
    2285264
  • 财政年份:
    2019
  • 资助金额:
    $ 49.3万
  • 项目类别:
    Studentship
Single-molecule and super-resolution imaging methods with maximum photon efficiency, increased spatiotemporal resolution and high detection sensitivity in densely crowded environments
单分子和超分辨率成像方法,在密集拥挤的环境中具有最大光子效率、更高的时空分辨率和高检测灵敏度
  • 批准号:
    9809804
  • 财政年份:
    2019
  • 资助金额:
    $ 49.3万
  • 项目类别:
Single-molecule and super-resolution imaging methods with maximum photon efficiency, increased spatiotemporal resolution and high detection sensitivity in densely crowded environments
单分子和超分辨率成像方法,在密集拥挤的环境中具有最大光子效率、更高的时空分辨率和高检测灵敏度
  • 批准号:
    10005376
  • 财政年份:
    2019
  • 资助金额:
    $ 49.3万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了