MRI: Acquisition of a fluorescence activated cell sorter

MRI：获取荧光激活细胞分选仪

• 批准号: 1337787
• 负责人: Dacheng Ren
• 金额: $ 45.9万
• 依托单位: Syracuse University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1337787&HistoricalAwards=false
• 关键词: MRI; Acquisition; fluorescence; activated; cell

1337787RenThis proposal requests funding for the acquisition of a state-of-the-art fluorescence activated cell (FAC) sorter. Syracuse University (SU) does not currently have a FAC sorter and this is a limiting factor for many research projects in different SU departments. The selected model is a Special FACS AriaTM system with 4 lasers from BD Biosciences. It fulfills the needs of all confirmed users and has sufficient flexibility to successfully incorporate future users. It will enable researchers to sort both prokaryotic and eukaryotic cells between 0.2 - 100 ìm in diameter with high speeds (up to 70,000 cells per second) and high accuracy (99%). This major piece of advanced instrumentation will be a crucial resource for many ongoing projects at SU in the areas of biomaterials (including cell-surface interactions, drug delivery, and nanotechnology), molecular and cell biology (including neuroscience, cell physiology, development, and inter-kingdom signaling) and biofuels research involving algae cells.

1337787ren本提案要求为购买最先进的荧光活化细胞（FAC）分选机提供资金。雪城大学目前没有FAC分类器，这是雪城大学不同院系的许多研究项目的限制因素。所选择的模型是一个特殊的FACS AriaTM系统，带有BD生物科学公司的4个激光器。它满足所有已确认用户的需求，并具有足够的灵活性，可以成功地纳入未来的用户。它将使研究人员能够以高速（每秒高达70,000个细胞）和高精度（99%）对直径在0.2 - 100 ìm之间的原核和真核细胞进行分类。这一重要的先进仪器将成为苏大生物材料（包括细胞表面相互作用、药物传递和纳米技术）、分子和细胞生物学（包括神经科学、细胞生理学、发育和跨界信号）和涉及藻类细胞的生物燃料研究领域许多正在进行的项目的关键资源。