MRI: Acquisition of a MALDI-TOF Mass Spectrometer

凭借由化学研究仪器和设施（CRIF）计划共同资助的化学主要研究仪器计划的这一奖项，波士顿大学（BU）的Mark Grinstaff教授将获得微型MALDI-TOF质谱仪（微型MALDI-TOF）。该提案旨在加强各级研究和教育，特别是在以下领域：（a）二血红素细胞色素c过氧化物酶的电化学;（B）抑制病原性生物膜的疏菌涂层;（c）多环芳烃（PAH）带的合成和反应性;（d）改进纳米颗粒功能化策略;和（e）蛋白质-蛋白质相互作用的抑制剂和活化剂的研究质谱法（MS）是用于鉴定和表征嵌入复杂基质中的少量化学物质的关键分析方法之一。在典型的实验中，组分流入质谱仪，在那里它们被电离成母离子及其碎片离子，并测量它们的质量。这种高灵敏度的技术可以检测和确定复杂混合物中分子的结构。BU Charles River校区目前的质谱能力存在一个关键的差距，因为没有支持生命过程、化学和纳米技术中的化学研究所需的仪器。查尔斯河校区的新仪器将直接和立即影响和加强40多名研究生，10名本科生和10名博士后研究人员的项目。新仪器将提供给BU的其他科学家和教育工作者，以及外部机构，如Emmanuel College，BU与他们合作推进教育和研究。

With this award from the Chemistry Major Research Instrumentation Program that is co-funded by the Chemistry Research Instrumentation and Facilities (CRIF) Program, Professor Mark Grinstaff from Boston University (BU) will acquire a micro-MALDI-TOF mass spectrometer (micro-MALDI-TOF). The proposal is aimed at enhancing research and education at all levels, especially in areas such as (a) electrochemistry of diheme cytochrome c peroxidases; (b) bacteriophobic coatings for inhibition of pathogenic biofilms; (c) synthesis and reactivity of polyaromatic hydrocarbon (PAH) belts; (d) improvement of nanoparticle functionalization strategies; and (e) studies of inhibitors and activators of protein-protein interactions.Mass Spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. A critical gap exists in the current mass spectrometric capabilities on the BU Charles River Campus because there is no instrument of the type needed to support chemical research in the life processes, chemistry and nanotechnology. The new instrument on the Charles River Campus would directly and immediately impact and enhance the projects of more than 40 graduate, 10 undergraduate, and 10 post-doctoral researchers. The new instrument would be available to other scientists and educators at BU, as well as outside institutions such as Emmanuel College, with whom BU has partnered in advancing education and research.