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Role of the CLE40-ACR4 signalling module in Arabidopsis root development

CLE40-ACR4信号模块在拟南芥根发育中的作用

基本信息

批准号：
214100624
负责人：
Professor Dr. Rüdiger Simon
金额：
--
依托单位：
Institut Entwicklungsgenetik
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2012
资助国家：
德国
起止时间：
2011-12-31 至 2014-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/214100624?language=en
关键词：
Role CLE40 ACR4 signalling module

项目摘要

The growth of plant roots is controlled by meristems that constantly generate new cells. These cells later specialize for their individual tasks, such as nutrient uptake, water transport, or protection of the growing root tip. The Arabidopsis root consists of cells arranged in regular cell files, which originate from stem cells at the root tip. Divisional activity of stem cells and differentiation of their daughter cells has to be coordinated, which is achieved through the exchange of signals such as the phytohormone auxin and small peptides. The peptide CLE40 is secreted by differentiated columella cells. It is perceived by the receptor protein ACR4 that resides on the plasma membrane of columella stem cells and controls expression of the transcription factor WOX5. WOX5 is in turn required to inhibit the columella stem cells from premature differentiation. Together, CLE40, ACR4 and WOX5 establish a regulatory module that controls cell behavior in the root meristem. We intend to analyse how the components of this module interact, their intersection with auxin signaling pathways, and the target genes that are regulated by this module and ultimately control a cells decision to further divide or differentiate. To understand the functioning of the CLE40-ACR4-WOX5 module, we will change the activity of the individual components, study their mutual transcription regulation, and investigate transcriptional control regions in their promoters. Input from hormone signalling pathways will be investigated by hormone treatments or inhibiton of hormone function and transport. Target genes and their activity will be identified by next-generation sequencing approaches, and by the generation of appropriate mutant lines.

植物根的生长是由不断产生新细胞的分生组织控制的。这些细胞后来专门从事各自的任务，如营养吸收，水分运输或保护生长的根尖。拟南芥的根由排列成规则细胞列的细胞组成，这些细胞起源于根尖的干细胞。干细胞的分裂活动和它们的子细胞的分化必须协调，这是通过交换信号如植物激素生长素和小肽来实现的。肽CLE 40由分化的鼻小柱细胞分泌。它由位于鼻小柱干细胞质膜上的受体蛋白ACR 4感知，并控制转录因子WOX 5的表达。WOX 5反过来又需要抑制小柱干细胞的过早分化。CLE 40、ACR 4和WOX 5共同建立了控制根分生组织中细胞行为的调控模块。我们打算分析这个模块的组件如何相互作用，它们与生长素信号通路的交叉，以及由这个模块调节并最终控制细胞进一步分裂或分化的靶基因。为了了解CLE 40-ACR 4-WOX 5模块的功能，我们将改变单个组件的活性，研究它们的相互转录调节，并研究它们的启动子中的转录控制区域。来自激素信号通路的输入将通过激素处理或激素功能和运输的研究来研究。目标基因及其活性将通过下一代测序方法和产生适当的突变系来鉴定。