Regulation of developmental pathways by the JAGGED LATERAL ORGANS gene of Arabidopsis

拟南芥 JAGGED LATERAL ORGANS 基因对发育途径的调节

• 批准号: 57771869
• 负责人: Professor Dr. Rüdiger Simon
• 金额: --
• 依托单位: Division of Plant and Crop Sciences
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/57771869?language=en
• 关键词: Regulation; developmental; pathways; JAGGED; LATERAL

Continuous growth and organ production from plant meristems requires a precise coordination of stem cell proliferation and commitment of stem cell descendents to diverse differentiation pathways. In Arabidopsis thaliana, KNOX-family homeodomain transcription factors that promote the undifferentiated state, and auxin transport and signaling pathways activated during organ initation appear to play antagonistic roles in the coordination of meristem development. In the first funding period, we showed that JAGGED LATERAL ORGANS (JLO), a transcription factor of the LATERAL ORGAN BOUNDARY DOMAIN (LBD) family, regulates the expression of both KNOX genes and genes of the PINFORMED (PIN) family which encode auxin efflux carrier. JLO interacts with other LBD proteins, such as AS2, and has a central regulatory function in multiple developmental pathways. We will now employ biochemical and molecular genetic approaches to characterise the protein complexes that involve JLO, identify the target genes and their functions which are regulated by JLO complexes, and finally ask how JLO itself, which is required for plant development from early embryogenesis onwards, is transcriptionally and functionally regulated.

植物分生组织的持续生长和器官产生需要干细胞增殖的精确协调和干细胞后代的不同分化途径的承诺。在拟南芥中，促进未分化状态的knox家族同源结构域转录因子和器官启动过程中激活的生长素运输和信号通路似乎在分生组织发育的协调中起拮抗作用。在第一个资助期，我们发现JAGGED LATERAL ORGANS （JLO）是一个侧面器官边界域（LBD）家族的转录因子，可以调节编码生长素外排载体的KNOX基因和PINFORMED （PIN）家族基因的表达。JLO与其他LBD蛋白相互作用，如AS2，并在多种发育途径中具有中心调节功能。我们现在将采用生物化学和分子遗传学的方法来表征涉及JLO的蛋白质复合物，确定受JLO复合物调节的靶基因及其功能，并最终询问从早期胚胎发生开始的植物发育所需的JLO本身是如何被转录和功能调节的。

项目成果

How boundaries control plant development.

• DOI: 10.1016/j.pbi.2013.11.013
• 发表时间: 2014-02
• 期刊: Current opinion in plant biology
• 影响因子: 9.5
• 作者: P. Zadnikova;R. Simon