Regulation of developmental pathways by the JAGGED LATERAL ORGANS gene of Arabidopsis

植物分生组织的持续生长和器官生产需要精确协调干细胞增殖和干细胞后代对不同分化途径的承诺。在拟南芥中，促进未分化状态的KNOX家族同源结构域转录因子，以及器官启动过程中激活的生长素运输和信号通路，似乎在分生组织发育的协调中起着拮抗作用。在第一个资助阶段，我们发现锯齿状侧方器官(JLO)是侧方器官边界结构域(LBD)家族的一个转录因子，它调节着KNOX基因和PINFORMED(PIN)家族基因的表达，PINFORMED家族编码生长素外流载体。JLO与其他LBD蛋白相互作用，如AS2，在多种发育途径中具有中枢调节功能。我们现在将利用生化和分子遗传学的方法来鉴定涉及JLO的蛋白质复合体，确定JLO复合体调控的靶基因及其功能，最后询问JLO本身是如何转录和功能调节的，从早期胚胎发生到植物发育都需要JLO。

Continuous growth and organ production from plant meristems requires a precise coordination of stem cell proliferation and commitment of stem cell descendents to diverse differentiation pathways. In Arabidopsis thaliana, KNOX-family homeodomain transcription factors that promote the undifferentiated state, and auxin transport and signaling pathways activated during organ initation appear to play antagonistic roles in the coordination of meristem development. In the first funding period, we showed that JAGGED LATERAL ORGANS (JLO), a transcription factor of the LATERAL ORGAN BOUNDARY DOMAIN (LBD) family, regulates the expression of both KNOX genes and genes of the PINFORMED (PIN) family which encode auxin efflux carrier. JLO interacts with other LBD proteins, such as AS2, and has a central regulatory function in multiple developmental pathways. We will now employ biochemical and molecular genetic approaches to characterise the protein complexes that involve JLO, identify the target genes and their functions which are regulated by JLO complexes, and finally ask how JLO itself, which is required for plant development from early embryogenesis onwards, is transcriptionally and functionally regulated.