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Role of the plasmodesmata localized ACR4 and CLV1 receptor kinases in controlling stem cell fate in the root

胞间连丝定位的 ACR4 和 CLV1 受体激酶在控制根干细胞命运中的作用

基本信息

批准号：
269106198
负责人：
Professor Dr. Rüdiger Simon
金额：
--
依托单位：
Institut Entwicklungsgenetik
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2014
资助国家：
德国
起止时间：
2013-12-31 至 2018-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/269106198?language=en
关键词：
Role plasmodesmata localized ACR4 CLV1

项目摘要

The homeostasis of the root stem cell domain is achieved by signaling between the quiescent centre (QC), stem cells and the surrounding cells that enter differentiation. It is not yet understood how cells communicate with each other in order to fine tune the balance between proliferation and differentiation. Recent work showed that the membrane associated receptor kinases ACR4 and CLV1, which control stem cell number in the distal root meristem, interact at the sites of plasmodesmata, suggesting that ACR4-CLV1 complexes may regulate the movement of signaling molecules necessary for root meristem homeostasis. In this project we plan to analyze if, and how, ACR4-CLV1 complexes control connectivity between meristem cells, and how this regulation contributes to the maintenance of root homeostasis. Furthermore, we will elucidate the downstream targets of ACR4-CLV1 complexes after perception of the CLE40 peptide. Therefore, we will perform transcript profiling of the QC in comparison with sorted columella stem cells, with or without CLE40 peptide treatment. Additionally, we will employ a genetic screen to identify new components regulating the balance between proliferation and differentiation. Ensuing gene cloning and functional analysis will expand our understanding of the ACR4-CLV1 signalling pathway. The results obtained in the proposed project will help the field in understanding how spatiotemporal communication integrates stem cell behaviour in the root meristem. Thus, this research project will address a fundamental problem in biology: how short range patterns of cells with different identities are generated and maintained, and how cell proliferation and differentiation is controlled by local signaling and is integrated with the growth requirements of the entire organism. Furthermore, understanding the longevity of plant stem niches will allow researchers to improve in general plant growth and biomass production.

根干细胞区域的动态平衡是通过静止中心(QC)、干细胞和进入分化的周围细胞之间的信号来实现的。目前还不清楚细胞之间是如何相互通信以微调增殖和分化之间的平衡的。最近的工作表明，控制远端根分生组织中干细胞数量的膜相关受体激酶ACR4和CLV1在胞间连丝位置相互作用，表明ACR4-CLV1复合体可能调节根分生组织动态平衡所必需的信号分子的运动。在这个项目中，我们计划分析ACR4-CLV1复合体是否以及如何控制分生组织细胞之间的连接，以及这种调节如何有助于维持根的动态平衡。此外，我们将阐明ACR4-CLV1复合体在感知CLE40肽后的下游靶点。因此，我们将对QC进行转录谱分析，并与分选的小柱干细胞进行比较，无论是否使用CLE40多肽处理。此外，我们将利用基因筛查来确定调节增殖和分化之间平衡的新成分。随后的基因克隆和功能分析将扩大我们对ACR4-CLV1信号通路的理解。在拟议的项目中获得的结果将有助于该领域理解时空通信如何将根分生组织中的干细胞行为结合在一起。因此，这一研究项目将解决生物学中的一个基本问题：如何产生和维持不同身份的细胞的短期模式，以及细胞的增殖和分化如何由局部信号控制并与整个生物体的生长要求相结合。此外，了解植物茎生态位的寿命将使研究人员从总体上改善植物生长和生物量生产。