Nanostructured Surfaces with Selective Biotraps for Sensing

具有用于传感的选择性生物陷阱的纳米结构表面

• 批准号: 1401720
• 负责人: Vladimir Tsukruk
• 金额: $ 30万
• 依托单位: Georgia Tech Research Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-15 至 2017-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1401720&HistoricalAwards=false
• 关键词: Nanostructured; Surfaces; Selective; Biotraps; Sensing

PI: Tsukruk, Vladimir V.Proposal: 1401720Title: Nanostructured Surfaces with Selective Biotraps for SensingThe demand for robust, reliable, selective, and sensitive detection of important stress or disease biomarkers has become high to meet the needs of the fast growing fields of biosensing for health, performance, and bioenvironmental monitoring as well as real-time monitoring of cognitive function, state of fatigue, and environmental exposure. Highly sensitive and selective nanomaterials for novel biosensing routines will be advanced in this project through precise fabrication of nanomaterials composed of noble metal nanostructures decorated with grafted selective biomolecules. A broader impact of this project is anticipated through the enhanced training of students with an emphasis on their early involvement in an interdisciplinary, fundamental & applied research experience. In addition, the PI places continued emphasis on recruiting under-represented students by enhancing collaboration with HBCU Morehouse College in Atlanta.Highly sensitive and selective hybrid nanomaterials for biosensing will be advanced in this project through an understanding of the selective binding characteristics of functionalized ?biotraps? composed of noble metal nanostructures decorated with grafted biomolecules. These biosensing nanomaterials will target stress-, performance-, and disease-related biomarkers based upon utilization of SERS phenomena. The candidates for biofunctionalization of metal nanostructures are aptamers and selectively-binding peptides, each capable of controlled grafting to the nanostructures and remarkable specificity to target molecules. Anisotropic individual plasmonic structures (such as gold and silver nanocubes) will be the materials base because their pattern of plasmonic resonances covers a wide range of the electromagnetic spectrum. Specific technical tasks to be addressed include: 1) fabrication of noble metal nanostructures with highly controlled shapes in array-form using electron beam lithography and directed self-assembly; 2) determine how specific structural peculiarities affect their individual plasmonic responses; 3) robust biofunctionalization of nanostructured gaps; 4) investigate SERS phenomena of these biofunctionalized traps designed to selectively bind different biomolecules. The highly interdisciplinary nature of the proposed research requires intense collaboration with biochemist experts from the Air Force Research Lab and chemists from academic institutions.

主要研究者：Tsukruk，弗拉基米尔五.建议：1401720标题：具有用于传感的选择性生物陷阱的纳米结构表面对重要压力或疾病生物标志物的稳健、可靠、选择性和灵敏检测的需求已经变得很高，以满足用于健康、性能和生物环境监测以及认知功能、疲劳状态和环境暴露的实时监测的快速增长的生物传感领域的需求。 本项目将通过精确制造由接枝选择性生物分子修饰的贵金属纳米结构组成的纳米材料，推进用于新型生物传感例程的高灵敏度和选择性纳米材料。 通过加强对学生的培训，强调他们早期参与跨学科的基本应用研究经验，预计该项目将产生更广泛的影响。 此外，PI的地方继续强调通过加强与HBCU莫尔豪斯学院在亚特兰大的合作招收代表性不足的学生。高灵敏度和选择性的生物传感杂化纳米材料将在这个项目中通过对功能化的选择性结合特性的理解先进？生物陷阱由修饰有接枝生物分子的贵金属纳米结构组成。 这些生物传感纳米材料将基于Sers现象的利用针对压力，性能和疾病相关的生物标志物。 金属纳米结构的生物功能化的候选物是适体和选择性结合肽，每种适体和选择性结合肽都能够受控地接枝到纳米结构上并且对靶分子具有显著的特异性。 各向异性的单个等离子体结构（如金和银纳米立方体）将是材料基础，因为它们的等离子体共振模式覆盖了宽范围的电磁波谱。 要解决的具体技术任务包括：1）使用电子束光刻和定向自组装制造具有高度可控形状的阵列形式的贵金属纳米结构; 2）确定特定结构特性如何影响其个体等离子体响应; 3）纳米结构间隙的鲁棒生物功能化; 4）研究这些生物功能化陷阱的Sers现象，这些陷阱旨在选择性地结合不同的生物分子。 拟议研究的高度跨学科性质需要与空军研究实验室的生物化学家和学术机构的化学家密切合作。