MRI: Development of a Multi-Photon Microscope with Adaptive Optics

MRI：开发具有自适应光学器件的多光子显微镜

• 批准号: 1429810
• 负责人: Marco Rolandi
• 金额: $ 50.38万
• 依托单位: University of California-Santa Cruz
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2020-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1429810&HistoricalAwards=false
• 关键词: MRI; Development; Multi; Photon; Microscope

An award is made to the University of California Santa Cruz to add long-wavelength excitation light (1,550 nm) and adaptive optics (AO) into a commercial multiphoton microscope (Olympus) for live deeptissue biological imaging. Adaptive optics has provided diffraction-limited images for large ground-based telescopes by correcting for the blurring caused by the atmosphere. They propose to re-apply similar adaptive optics techniques in multiphoton microscopy to obtain diffraction-limited images from deep within living biological tissues. This development will provide biologists with new imaging capabilities, similar to advantages adaptive optics provided to astronomers, enabling them to obtain the highestresolution deep-tissue images in neurobiology to study brain development and synapse plasticity in the nervous system, in cell biology to study cell cycle events, and in developmental biology to study stem cells, chromatin remodeling and the cytoskeleton. The research activities in this project include opportunities for undergraduate and graduate students to participate in a multidisciplinary research team consisting of optical physicists, electrical engineers, and biologists, contributing to their training as the next generation of instrumentalists and advanced users. The research activities and instrumentation will be integrated into teaching at the undergraduate and graduate levels through hands-on projects in engineering and science courses at UCSC, a Hispanic Serving Institution (HSI), and through an NSF sponsored outreach program, the California State Summer School for Mathematics and Science(COSMOS), a summer residential program for high school scholars with demonstrated interest and achievement in math and science. They will also work in a partnership with all ten UC campuses and three affiliated national labs (LANL, LBNL, and LLNL) and industry to broadly disseminate the technology to the biological research community. Such improvements will benefit society by greatly advancing our fundamental understanding of life processes at the cellular and sub-cellular levels.The proposed activity more than doubles the depth (1,500nm) of diffraction limited imaging (738 nm) into dynamic live tissue (AO frame rate 1 Hz) where many fundamental cellular processes occur, such as neuron growth, organization and synapse formation. For example, by increasing the imaging depth from 500 um to 1,000 um, they can reach the deeper cortical layers to see if synaptic reorganization during development and under pathological conditions follows similar rules as in the superficial cortical layers, were synapses are constantly remodeling in living animals. By increasing the imaging depth beyond 1,000 um they can reach the Hippocampus, the site for learning and memory. This will allow studies of how synapses reorganize during learning and how they encode for long-lasting memory.

加州大学圣克鲁斯分校获奖，将长波长激发光(1,550 nm)和自适应光学(AO)添加到商用多光子显微镜(奥林巴斯)中，用于活体深层组织生物成像。自适应光学通过校正大气造成的模糊，为大型地面望远镜提供了衍射受限的图像。他们建议将类似的自适应光学技术重新应用于多光子显微镜，以获得生物组织内部深处的衍射限制图像。这一进展将为生物学家提供新的成像能力，类似于自适应光学向天文学家提供的优势，使他们能够获得最高分辨率的深层组织图像，在神经生物学中研究大脑发育和神经系统中的突触可塑性，在细胞生物学中研究细胞周期事件，在发育生物学中研究干细胞，染色质重塑和细胞骨架。该项目的研究活动包括让本科生和研究生有机会参加由光学物理学家、电气工程师和生物学家组成的多学科研究团队，为他们作为下一代仪器手和高级用户的培训做出贡献。研究活动和工具将整合到本科生和研究生的教学中，通过南加州大学(UCSC)的工程和科学课程实践项目，以及由NSF赞助的加州州立数学与科学暑期学校(COSMOS)，这是一个针对对数学和科学表现出兴趣和成就的高中学者的暑期住宿计划。他们还将与加州大学所有十个校区和三个附属国家实验室(LANL、LBNL和LLNL)和行业合作，向生物研究界广泛传播这项技术。这些改进将极大地促进我们在细胞和亚细胞水平上对生命过程的基本理解，从而使社会受益。拟议的活动将衍射限制成像(738 Nm)的深度(1500 Nm)增加一倍以上，进入动态活组织(AO帧速率1赫兹)，许多基本的细胞过程发生，如神经元的生长、组织和突触形成。例如，通过将成像深度从500微米增加到1,000微米，他们可以到达更深的皮质层，以查看在发育过程中和病理条件下的突触重组是否遵循与皮层浅层相似的规则，因为突触在活体动物中不断重塑。通过增加成像深度超过1000微米，他们可以到达海马体，学习和记忆的地方。这将允许研究突触在学习过程中如何重组，以及它们如何编码以实现持久记忆。