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CAREER: Surface and Interparticle Forces for Improved Virus Removal

职业：表面力和颗粒间力可改善病毒去除效果

基本信息

批准号：
1451959
负责人：
Caryn Heldt
金额：
$ 52.57万
依托单位：
Michigan Technological University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2015
资助国家：
美国
起止时间：
2015-05-01 至 2022-04-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1451959&HistoricalAwards=false
关键词：
CAREER Surface Interparticle Forces Improved

项目摘要

1451959HeldtThis CAREER project proposes a shift in the design and creation of next-generation virus removal and detection technologies. Instead of screening large libraries of chemistries to determine the ones that will most efficiently remove viruses, this project will first examine the surface chemistry of the viruses. This will lead to targeted chemistries that will attract viruses and exponentially increase the efficiency of screening processes. The process to be followed will benchmark new surface evaluation techniques with currently established surface chemistry analysis to determine if chemical forces of viruses can be determined at the single-particle scale. The CAREER education plan will train undergraduate and graduate students to be role models to high school and community college students in order to attract and retain underrepresented minorities to STEM fields. The main focus of the education plan is a collaboration with Wayne County Community College in Detroit, MI to provide engaging and hands-on research opportunities to community college students. The goal is to use research as a vehicle for sustained STEM engagement and to encourage B.S. degree completion. This unique program will expose over 500 inner city students to research opportunities and expanded career options. The proposed work is built upon the hypothesis that an advanced understanding of viral surface chemical interactions and interparticle forces will enable a significant transformation in virus removal and detection technologies, dramatically reducing the cost while simultaneously increasing efficiency. The outcome may result in an increase in availability and accessibility to life-saving biotherapies. The overall goal of this CAREER project is to transform virus removal processes by creating a fundamental framework of viral surface and interparticle forces. The specific goals to be accomplished are: 1) Analyze the surface chemistry of several model viruses, 2) Quantify interparticle forces of model viruses and 3) Prototype a surface and interparticle force-informed method for virus removal. This study of surface and interparticle forces will benchmark traditional techniques against novel surface characterizations. Quantitative surface chemistry and interparticle force data will inform an innovative design of virus removal processes. Three relevant viruses will be used in this study, an enveloped mammalian virus, a non-enveloped mammalian virus and a bacteriophage.

1451959heldtcareer项目提出了下一代病毒清除和检测技术的设计和创造方面的转变。这个项目将首先检查病毒的表面化学成分，而不是筛选大型化学成分库来确定最有效地去除病毒的化学成分。这将导致有针对性的化学物质吸引病毒，并成倍提高筛选过程的效率。接下来的过程将以目前建立的表面化学分析作为新的表面评估技术的基准，以确定是否可以在单颗粒尺度上确定病毒的化学力。职业教育计划将培养本科生和研究生成为高中和社区大学学生的榜样，以吸引和留住代表性不足的少数民族进入STEM领域。教育计划的主要重点是与密歇根州底特律的韦恩县社区学院合作，为社区大学生提供参与和实践的研究机会。目标是利用研究作为持续参与STEM的工具，并鼓励完成学士学位。这个独特的项目将为500多名内城学生提供研究机会和更多的职业选择。提出的工作是建立在这样一个假设之上的：对病毒表面化学相互作用和粒子间力的深入了解将使病毒去除和检测技术发生重大转变，大大降低成本，同时提高效率。其结果可能导致救生生物疗法的可得性和可及性的增加。这个CAREER项目的总体目标是通过创建病毒表面和粒子间力的基本框架来改变病毒去除过程。具体目标是：1)分析几种模型病毒的表面化学性质；2)量化模型病毒的粒子间力；3)原型化一种表面和粒子间力信息的病毒去除方法。这项表面和粒子间力的研究将以传统技术为基准，对抗新的表面表征。定量表面化学和粒子间力数据将为病毒去除过程的创新设计提供信息。本研究将使用三种相关病毒，包膜哺乳动物病毒，非包膜哺乳动物病毒和噬菌体。

项目成果

期刊论文数量（11）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Single-particle chemical force microscopy to characterize virus surface chemistry

DOI：
10.2144/btn-2020-0085
发表时间：
2020-10-01
期刊：
BIOTECHNIQUES
影响因子：
2.7
作者：
Mi, Xue;Heldt, Caryn L.
通讯作者：
Heldt, Caryn L.

Virus Isoelectric Point Determination Using Single-Particle Chemical Force Microscopy

DOI：
10.1021/acs.langmuir.9b03070
发表时间：
2020-01-14
期刊：
LANGMUIR
影响因子：
3.9
作者：
Mi, Xue;Bromley, Emily K.;Heldt, Caryn L.
通讯作者：
Heldt, Caryn L.

Challenges in downstream purification of gene therapy viral vectors

DOI：
10.1016/j.coche.2021.100780
发表时间：
2022-03-01
期刊：
CURRENT OPINION IN CHEMICAL ENGINEERING
影响因子：
6.6
作者：
Singh, Nripen;Heldt, Caryn L.
通讯作者：
Heldt, Caryn L.

A generalized purification step for viral particles using mannitol flocculation

DOI：
10.1002/btpr.2651
发表时间：
2018-07
期刊：
Biotechnology Progress
影响因子：
2.9
作者：
C. Heldt;Ashish Saksule;Pratik U. Joshi;M. Ghafarian
通讯作者：
C. Heldt;Ashish Saksule;Pratik U. Joshi;M. Ghafarian

Osmolyte enhanced aqueous two‐phase system for virus purification

用于病毒纯化的渗透剂增强水两相系统

DOI：
10.1002/bit.27849
发表时间：
2021
期刊：
Biotechnology and Bioengineering
影响因子：
3.8
作者：
Joshi, Pratik U.;Turpeinen, Dylan G.;Schroeder, Michael;Jones, Bianca;Lyons, Audrey;Kriz, Seth;Khaksari, Maryam;O'Hagan, David;Nikam, Savita;Heldt, Caryn L.
通讯作者：
Heldt, Caryn L.