EAGER: Engineering bacterial lectins for glycomics analysis

EAGER：工程细菌凝集素用于糖组学分析

• 批准号: 1452290
• 负责人: Ruizhen Chen
• 金额: $ 4.04万
• 依托单位: Georgia Tech Research Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1452290&HistoricalAwards=false
• 关键词: EAGER; Engineering; bacterial; lectins; glycomics

1452290ChenGeorgia TechGlycosylation is the process that attaches glycans to proteins, lipids, or other organic molecules. Glycans on proteins and lipids may serve as markers or identifiers in many vital biological processes such as cell growth, cell-cell interactions,and cell mig. Unusual glycosylation has been implicated in many different types of diseases including cancer. The lack of simple and effective tools to detect glycolsylation creates a significant obstacle to the advances in this area. The PI proposes a study to develop new tools and methods useful to assist in the detection of glycolsylation that are low cost and simple to use. Two specific aims are proposed: Aim 1: the PI proposes to develop and validate a novel glycoprotein quantification method, which can be incorporated into commonly used protein separation techniques. If successful the work proposed in could extend the use of 1-D and 2-D electrophoresis separation techniques. It could also make these tools more reliably employed and useful tool for glycoscience. Aim 2: As an example the PI proposes to demonstrate that the developed methods could be used for a real-time label-free bacterial surface glycome profiling and for study of dynamics of bacterial glycosylation in response to environmental conditions. The proposal also plans to incorporate engineered lectin molecules into the common protein separation and analysis format, such as SDS gel electrophoresis. If successful, this will change how glycans are detected by eliminating the need for antibody and other expensive reagents commonly in the processes. The proposed research may advance the development of much needed tools to simplify methods commonly used in glycoscience research. The success of the research has the potential to make glycomic analysis less arduous and less time consuming, less labor and material intensive. As a result, glycomic analysis may no longer be conducted by only a small number of specialized laboratories and may become much more accessible to a common laboratory. Thus the research will have significant impact on the field by bringing low cost tools to glycoscience researchers.

1452290 ChenGeorgia TechGlycosylation是将聚糖连接到蛋白质、脂质或其他有机分子上的过程。蛋白质和脂质上的聚糖在许多重要的生物过程中，如细胞生长、细胞间相互作用和细胞mig中，可以作为标记物或标识物。不寻常的糖基化与包括癌症在内的许多不同类型的疾病有关。缺乏简单有效的检测糖基化的工具对这一领域的进展造成了重大障碍。PI提出了一项研究，旨在开发有助于检测糖基化的新工具和方法，这些工具和方法成本低且易于使用。提出了两个具体目标：目标1：PI提出开发和验证一种新型糖蛋白定量方法，该方法可纳入常用的蛋白质分离技术。如果成功的工作中提出的可以扩展使用1-D和2-D电泳分离技术。它也可以使这些工具更可靠地使用和有用的工具，糖科学。目标二：作为一个例子，PI建议证明所开发的方法可用于实时无标记细菌表面糖组分析和研究细菌糖基化响应环境条件的动力学。该提案还计划将工程凝集素分子纳入常见的蛋白质分离和分析格式，如SDS凝胶电泳。如果成功，这将通过消除对抗体和其他昂贵试剂的需求来改变聚糖的检测方式。拟议的研究可能会促进急需的工具的开发，以简化糖科学研究中常用的方法。这项研究的成功有可能使糖组学分析变得不那么费力，耗时更少，劳动力和材料密集度更低。因此，糖组学分析可能不再仅由少数专业实验室进行，而可能变得更容易由普通实验室进行。因此，该研究将通过为糖科学研究人员带来低成本工具而对该领域产生重大影响。