CSBR: Living Stocks - The Escherichia Coli Genetic Stock Center

CSBR：活体种群 - 大肠杆菌遗传种群中心

• 批准号: 1458262
• 负责人: John Wertz
• 金额: $ 49.87万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-15 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1458262&HistoricalAwards=false
• 关键词: CSBR; Living; Stocks; Escherichia; Coli

This award supports the E. coli Genetic Stock Center (CGSC) at Yale University. Escherichia coli is an important model organism and is perhaps the best understood cellular organism from a genetics and biochemistry standpoint. This high degree of understanding has made E. coli not just an important subject of research, but also the laboratory workhorse for studying a broad variety of organisms as well as an invaluable tool for biotechnology. The mission of the CGSC is to act as a central repository for genetically modified strains of E. coli created by the research community, so that they may be readily available to other researchers and educators. To that end, the CGSC maintains a collection of ~28,000 cultures of genetically defined derivatives of E. coli K-12. There are over 8,000 different mutations represented in this strain collection with verified mutations in over 90% of the 4,111 identified genes in the sequenced E. coli K-12 genome. These cultures are distributed to researchers and educators in the US and over 65 countries worldwide. In addition to the distribution of cultures, the CGSC is also an information resource, and provides information about strains, mutations, genes, gene products and genetic maps, in a fully searchable database on the web. Importantly CGSC also acts as a source of expert information and advice for those researchers who have questions about using the E. coli strains in the collection. The CGSC will continue to maintain and expand its publicly available database on strain genotypes, phenotypes, histories and pedigrees, which is unique to the Stock Center. Information systems will be upgraded to more seamlessly integrate with other E. coli web resources, including the EcoliWiki project, EcoGene and EcoCyc. The CGSC is collaborating with other international culture collection databases such as StrainInfo and the World Data Centre for Microorganisms (WDCM) so that the collection can be indexed by these global strain databases. Over the period of this award, capabilities will be expanded to distribute large systematic strain sets, which are useful for users interested in genomic and proteomic research. Strain construction services will be offered for those users who require a genetically modified strain, but lack the capability of constructing it themselves, particularly to those outside of the traditional bacterial genetics community. Access to the strain collection database, including genotypes, gene function and map location, as well as an extensive bibliography are available at (http://cgsc.biology.yale.edu/).

该奖项支持E。大肠杆菌遗传储备中心（CGSC）在耶鲁大学。大肠杆菌是一种重要的模式生物，从遗传学和生物化学的角度来看，可能是最好理解的细胞生物。这种高度的理解使E.大肠杆菌不仅是一个重要的研究课题，也是研究各种生物的实验室主力，也是生物技术的宝贵工具。CGSC的使命是作为转基因大肠杆菌菌株的中央储存库。大肠杆菌中创建的，以便他们可以随时提供给其他研究人员和教育工作者。为此，CGSC保存了约28，000个E. coli K-12。在该菌株集合中有超过8，000种不同的突变，在测序的E. coli K-12基因组。这些培养物被分发给美国和全球65个国家的研究人员和教育工作者。除了文化的分布，CGSC也是一个信息资源，并提供有关菌株，突变，基因，基因产物和遗传图谱的信息，在网上完全可搜索的数据库。重要的是，CGSC还为那些对使用E.大肠杆菌菌株的收集。CGSC将继续维护和扩大其关于菌株基因型、表型、历史和谱系的公开数据库，这是库存中心独有的。信息系统将得到升级，以更无缝地与其他电子商务系统集成。大肠杆菌网络资源，包括EcoliWiki项目，EcoGene和EcoCyc。 CGSC正在与其他国际培养物收集数据库合作，如StrainInfo和世界微生物数据中心（WDCM），以便这些全球菌株数据库可以索引这些收集。 在此期间，将扩大能力，以分发大型系统菌株集，这对对基因组学和蛋白质组学研究感兴趣的用户很有用。 菌株构建服务将提供给需要转基因菌株但缺乏自行构建能力的用户，特别是传统细菌遗传学界以外的用户。访问菌株收集数据库，包括基因型、基因功能和图谱位置，以及广泛的参考书目可在（http：//cgsc.biology.yale.edu/）上获得。