LSCBR: The Escherichia coli Genetic Stock Center

LSCBR：大肠杆菌遗传库存中心

• 批准号: 1049728
• 负责人: John Wertz
• 金额: $ 133.25万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-15 至 2016-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1049728&HistoricalAwards=false
• 关键词: LSCBR; Escherichia; coli; Genetic; Stock

This award renews support for the operation of the E. coli Genetic Stock Center (CGSC) at Yale University. Escherichia coli is an important model organism and is perhaps the best understood cellular organism from a genetics and biochemistry standpoint. This high degree of understanding has made E. coli not just an important subject of research, but also the laboratory workhorse for studying a broad variety of organisms as well as an invaluable tool for biotechnology. The CGSC maintains a collection of ca. 27,500 cultures of genetically defined derivatives of E. coli K-12. There are over 8,000 different mutations represented in the strain collection with verified mutations in over 90% of the 4,111 identified genes in the sequenced E. coli K-12 genome. These cultures are distributed to researchers and educators in the US and over 70 countries worldwide. The CGSC provides information about strains, genes, gene products and genetic maps in a fully searchable database at our website (http://cgsc.biology.yale.edu). Importantly CGSC also acts as a source of expert information and advice for those researchers who have questions about using E. coli strains. The CGSC provides cultures for educational purposes ranging from elementary school science fair projects to graduate courses in molecular genetics. It also supplies strains to researchers in industry, government and academic institutions studying a diverse range of subjects. The number of strains distributed by the Stock Center has increased 89% over the past 5 years and it is currently distributing ca. 7,000 strains annually in response to nearly 2,000 requests. Support provided through this award subsidizes ca. 54% of the direct costs of maintaining the Stock Center. User fees provide for the balance of the operating expenses.

该奖项重申了对E。大肠杆菌遗传储备中心（CGSC）在耶鲁大学。 大肠杆菌是一种重要的模式生物，从遗传学和生物化学的角度来看，可能是最好理解的细胞生物。 这种高度的理解使E.大肠杆菌不仅是一个重要的研究课题，也是研究各种生物的实验室主力，也是生物技术的宝贵工具。 CGSC保存了一个CA集合。27，500个E. coli K-12中表达。在菌株集合中有超过8，000种不同的突变，在测序的E. coli K-12基因组。 这些文化被分发给美国和全球70多个国家的研究人员和教育工作者。CGSC在我们的网站（http：//www.example.com）上提供了关于菌株、基因、基因产物和遗传图谱的信息，可完全检索。cgsc.biology.yale.edu 重要的是，CGSC还为那些对使用E.大肠杆菌菌株。CGSC为教育目的提供文化，从小学科学展览项目到分子遗传学研究生课程。 它还为工业、政府和学术机构研究各种学科的研究人员提供菌株。 在过去的5年里，库存中心分发的菌株数量增加了89%，目前正在分发约1000种。每年有7,000个菌株响应近2,000个请求。通过这个奖项提供的支持补贴CA。54%的直接成本维持库存中心。使用费用于支付业务费用的余额。