LSCBR: The Escherichia coli Genetic Stock Center

该奖项再次支持耶鲁大学大肠杆菌遗传储备中心（CGSC）的运作。大肠杆菌是一种重要的模式生物，从遗传学和生物化学的角度来看，它可能是最容易理解的细胞生物。这种高度的理解使得大肠杆菌不仅是一个重要的研究对象，而且是研究各种生物的实验室工具，也是生物技术的宝贵工具。CGSC保存了大约27,500种大肠杆菌K-12基因衍生物的培养物。菌株收集中有超过8000种不同的突变，在大肠杆菌K-12基因组测序的4111个已鉴定基因中，超过90%的基因已证实突变。这些文化被分发给美国和全球70多个国家的研究人员和教育工作者。CGSC在我们的网站（http://cgsc.biology.yale.edu）上提供菌株、基因、基因产物和基因图谱的信息。重要的是，CGSC还为那些对使用大肠杆菌菌株有疑问的研究人员提供专家信息和建议。CGSC为教育目的提供文化，从小学科学展览项目到研究生分子遗传学课程。它还为工业、政府和学术机构研究各种学科的研究人员提供了菌株。库存中心分发的菌株数量在过去5年中增加了89%，目前每年分发约7,000株，以响应近2,000个请求。通过该奖项提供的支持补贴了大约54%的维护库存中心的直接成本。用户费用用于支付运营费用的余额。

This award renews support for the operation of the E. coli Genetic Stock Center (CGSC) at Yale University. Escherichia coli is an important model organism and is perhaps the best understood cellular organism from a genetics and biochemistry standpoint. This high degree of understanding has made E. coli not just an important subject of research, but also the laboratory workhorse for studying a broad variety of organisms as well as an invaluable tool for biotechnology. The CGSC maintains a collection of ca. 27,500 cultures of genetically defined derivatives of E. coli K-12. There are over 8,000 different mutations represented in the strain collection with verified mutations in over 90% of the 4,111 identified genes in the sequenced E. coli K-12 genome. These cultures are distributed to researchers and educators in the US and over 70 countries worldwide. The CGSC provides information about strains, genes, gene products and genetic maps in a fully searchable database at our website (http://cgsc.biology.yale.edu). Importantly CGSC also acts as a source of expert information and advice for those researchers who have questions about using E. coli strains. The CGSC provides cultures for educational purposes ranging from elementary school science fair projects to graduate courses in molecular genetics. It also supplies strains to researchers in industry, government and academic institutions studying a diverse range of subjects. The number of strains distributed by the Stock Center has increased 89% over the past 5 years and it is currently distributing ca. 7,000 strains annually in response to nearly 2,000 requests. Support provided through this award subsidizes ca. 54% of the direct costs of maintaining the Stock Center. User fees provide for the balance of the operating expenses.