LSC: The Escherichia Coli Genetic Stock Center

该奖项为耶鲁大学大肠杆菌遗传储备中心（CGSC）的运作提供了新的支持。大肠杆菌是一种重要的模式生物，从遗传学和生物化学的角度来看，它可能是最容易理解的细胞生物。这种高度的理解使得大肠杆菌不仅是一个重要的研究对象，而且是对各种各样的生物体进行分子遗传学研究的实验室工作马，也是生物技术的宝贵工具。CGSC保持了超过8500个大肠杆菌菌株K-12的基因定义衍生物的活跃收集。随着新的有用菌株的出现，这个集合经常更新，例如最近获得的Keio集合，一个超过3800个单基因敲除的系统集合。CGSC通常每年向全世界的科学家和教育工作者分发大约5000株菌株。此外，CGSC还在收集网站（http://cgsc.biology.yale.edu）上提供了有关现有菌株的信息、遗传图谱以及基因和基因产物的信息，这些信息在一个完全可搜索的数据库中。CGSC还参与了EcoliHub项目和其他基于网络的资源，如EcoGene和EcoCyc，这些资源直接链接到CGSC的数据库。作为其在研究界的作用的一部分，CGSC协调基因符号和等位基因编号的分配，并努力鼓励遵守公认的遗传命名法。通过该奖项提供的支持补贴了维持菌株收集和相应数据库的持续成本的约76%。用户费用和机构支持提供了运营费用的余额。

This award provides renewed support for the operation of the Escherichia coli Genetic Stock Center (CGSC) at Yale University. Escherichia coli (E. coli) is an important model organism and is perhaps the best understood cellular organism from a genetics and biochemistry standpoint. This high degree of understanding has made E. coli not just an important subject of research, but also the laboratory workhorse for molecular genetics research on a broad variety of organisms as well as an invaluable tool for biotechnology. The CGSC maintains an active collection of over 8,500 genetically defined derivatives of the E. coli strain K-12. This collection is updated frequently as new useful strains become available such as the recently acquired Keio collection, a systematic collection of over 3,800 single gene knockouts. The CGSC typically distributes approximately 5,000 strains per year to scientists and educators worldwide. In addition, the CGSC provides information about available strains, as well as genetic maps and information on genes and gene products in a fully searchable database at the collection website (http://cgsc.biology.yale.edu). The CGSC also participates in the EcoliHub project and other web based resources such as EcoGene, and EcoCyc that link directly to the CGSC database. As part of its role in the research community, the CGSC coordinates the assignment of gene symbols and allele numbers and works to encourage adherence to accepted genetic nomenclature. Support provided through this award subsidizes approximately 76% of the ongoing cost of maintaining the strain collection and the corresponding databases. User's fees and institutional support provide for the balance of the operating expenses.