Mapping and analysis of the infection-related dynamics of active DNA regions in the genome of Colletotrichum graminicola.

植物病原性子囊菌禾生炭疽菌（Colletotrichum graminicola）在世界范围内导致玉米种植的大量产量损失。除了经济上的重要性，C。草生菌已被确立为植物病理学中关于半活体营养型感染策略的模式生物。因此，该真菌代表炭疽菌属，其许多植物病原体感染广泛范围的农学上重要的作物和水果。禾生草属的形态和生理变化剧烈。成功的侵染依赖于宿主表面的识别和附着、孢子的萌发、附着胞的形成、分泌效应子对宿主防御和库源关系的调控以及侵入性坏死营养菌丝的分化。在许多真核生物中，分化受表观遗传调控，通过染色质的凝聚直接影响发育基因的转录活性。我们利用FAIREseq（Formaldehyde-Assisted Isolation of Regulatory Elements，甲醛辅助分离调控元件）技术，旨在鉴定和跟踪C.禾生菌在玉米病原发育过程中的作用。本项目的目标是鉴定表观遗传调控基因和基因组区域，这些基因和区域对于建立和维持相容的宿主-病原体相互作用非常重要。

The plant pathogenic ascomycete Colletotrichum graminicola causes substantial yield losses in maize cultivation worldwide. In addition to the economic importance, C. graminicola has been established as a model organism in plant pathology with respect to the hemibiotrophic infection strategy. Thus, the fungus represents the genus Colletotrichum whose numerous plant pathogens infect a broad range of agronomically important crops and fruits.Pathogenic development of the C. graminicola is featured by drastic morphological and physiological changes. Successful infection depends on recognition of the host surface and attachment, spore germination, formation of an appressorium, manipulation of host defense and sink-source relations by secreted effectors as well as differentiation of invasive necrotrophic hyphae.In many eukaryotic organisms, differentiation is controlled by epigenetic regulation via condensation of chromatin which directly affects transcriptional activity of developmental genes. It is still largely unknown whether this dynamic process is also involved in the infection-related morphogenesis of fungal pathogens or the expression of virulence genes.Using FAIREseq (Formaldehyde-Assisted Isolation of Regulatory Elements), we aim at identifying and following the dynamics of euchromatin in the genome of C. graminicola during pathogenic development in maize. The objective of the current project is the identification of epigenetically regulated genes and genomic regions that are important for establishing and maintaining a compatible host-pathogen interaction.