UNS:Coupling Thermophoresis with Engineered Convection for Label free, Continuous Bionanoparticle Concentration in Microfluidic Devices

1511284成都大学生物纳米颗粒，如病毒和囊泡，通常集中在临床诊断，国防监测和食品安全监测。常规方法，如高速离心和纳米过滤，是仪器和劳动密集型的，在资源有限的条件下不实用。这些挑战促使研究人员创造一种用于纳米颗粒加工的新型微流体解决方案。拟议研究的成功将对病毒样本处理产生广泛的实际影响，用于感染的临床诊断，国土安全监测和食品安全监测。 这种跨学科的研究将为本科生和研究生培训提供极好的机会。这项研究将有助于课程开发，并直接影响本科和研究生课程。调查人员将继续积极参与K-12外展计划，以激励和吸引有才华的学生到STEM领域。研究人员还将继续努力增加来自代表性不足群体的学生在这项研究计划中的参与。拟议的策略将热泳与工程对流相结合，以克服布朗运动，实现定向纳米粒子迁移。 所提出的策略是使纳米粒子迁移的温度梯度和合理设计的对流，以增加这种分离的基本调查。使用具有受控运动学和热分布的简单微尺度流，该方法对于生物溶液中的悬浮纳米级成分（例如病毒、脂质体和外来体）是通用的。分离过程是生物相容的，无标记的，并且浓缩的物质被连续地回收。除了与设计更快，更好，更便宜的诊断相关的变革性社会影响外，该研究还创新性地产生了对生物物种的非平衡运输和微结构基底无惯性流动中的流场控制的基本理解。

1511284ChengLehigh UniversityBionanoparticles, such as viruses and vesicles, are commonly concentrated in clinical diagnosis, defense surveillance and food safety monitoring. Conventional methods, such as high-speed centrifugation and nanofiltration, are instrument and labor intensive and unpractical under resource-limited conditions. These challenges motivate the researchers to create a novel microfluidic solution for nanoparticle processing. Success of the proposed research will have broad practical impact in viral sample processing for clinical diagnostics of infection, homeland security surveillance and food safety monitoring. This interdisciplinary research will provide excellent opportunities for undergraduate and graduate student training. This research will contribute to course development and directly impact the undergraduate and graduate curriculum. The investigators will continue to actively participate in K-12 outreach programs to motivate and attract talented students to STEM fields. The investigators will also continue the effort to increase the participation of students from underrepresented groups in this research program.The proposed strategy combines thermophoresis with engineered convection to overcome Brownian motion and achieve directed nanoparticle migration. The proposed strategy is enabled by a fundamental investigation of nanoparticle migration in a temperature gradient and rational design of convective flow to augment such separations. Using a simple microscale flow having controlled kinematics and thermal profiles, the approach is universal for suspended nanoscale constituents such as viruses, liposomes and exosomes in biological solutions. The separation process is biocompatible, label-free, and the concentrated species are retrieved continuously. In addition to the transformative societal impact associated with designing faster, better, cheaper diagnostics, the research is also innovative in generating fundamental understanding of both non-equilibrium transport of biological species and flow field control in inertial-free flows by microstructured substrates.