UNS:Coupling Thermophoresis with Engineered Convection for Label free, Continuous Bionanoparticle Concentration in Microfluidic Devices

1511284成都大学生物纳米粒子，如病毒和水泡，通常集中在临床诊断、防御监测和食品安全监测中。传统的方法，如高速离心法和纳滤法，都是仪器和劳动力密集型的，在资源有限的条件下不切实际。这些挑战促使研究人员创造一种用于纳米颗粒加工的新型微流控解决方案。拟议研究的成功将在病毒样本处理方面产生广泛的实际影响，用于感染的临床诊断、国土安全监测和食品安全监测。这种跨学科的研究将为本科生和研究生的培养提供极好的机会。这项研究将有助于课程的发展，并直接影响本科和研究生的课程。调查人员将继续积极参与K-12外展计划，以激励和吸引有才华的学生进入STEM领域。研究人员还将继续努力增加来自代表性不足群体的学生参与这一研究计划。建议的策略将热泳与工程对流相结合，以克服布朗运动，实现纳米粒子的定向迁移。提出的策略是通过对纳米颗粒在温度梯度中迁移的基础研究和合理的对流设计来增强这种分离。利用简单的微尺度流动控制运动学和热分布，该方法适用于生物溶液中悬浮的纳米级成分，如病毒、脂质体和外切体。分离过程是生物相容的，无需标记，并且浓缩的物种可以连续回收。除了与设计更快、更好、更便宜的诊断方法相关的变革性社会影响外，这项研究还创新地产生了对生物物种的非平衡传输和微结构基质在无惯性流动中的流场控制的基本理解。

1511284ChengLehigh UniversityBionanoparticles, such as viruses and vesicles, are commonly concentrated in clinical diagnosis, defense surveillance and food safety monitoring. Conventional methods, such as high-speed centrifugation and nanofiltration, are instrument and labor intensive and unpractical under resource-limited conditions. These challenges motivate the researchers to create a novel microfluidic solution for nanoparticle processing. Success of the proposed research will have broad practical impact in viral sample processing for clinical diagnostics of infection, homeland security surveillance and food safety monitoring. This interdisciplinary research will provide excellent opportunities for undergraduate and graduate student training. This research will contribute to course development and directly impact the undergraduate and graduate curriculum. The investigators will continue to actively participate in K-12 outreach programs to motivate and attract talented students to STEM fields. The investigators will also continue the effort to increase the participation of students from underrepresented groups in this research program.The proposed strategy combines thermophoresis with engineered convection to overcome Brownian motion and achieve directed nanoparticle migration. The proposed strategy is enabled by a fundamental investigation of nanoparticle migration in a temperature gradient and rational design of convective flow to augment such separations. Using a simple microscale flow having controlled kinematics and thermal profiles, the approach is universal for suspended nanoscale constituents such as viruses, liposomes and exosomes in biological solutions. The separation process is biocompatible, label-free, and the concentrated species are retrieved continuously. In addition to the transformative societal impact associated with designing faster, better, cheaper diagnostics, the research is also innovative in generating fundamental understanding of both non-equilibrium transport of biological species and flow field control in inertial-free flows by microstructured substrates.