EAPSI: Discovering Novel Functions of Protein Repeats Critical to Gene Expression

EAPSI:发现对基因表达至关重要的蛋白质重复的新功能

基本信息

  • 批准号:
    1514793
  • 负责人:
  • 金额:
    $ 0.51万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Fellowship Award
  • 财政年份:
    2015
  • 资助国家:
    美国
  • 起止时间:
    2015-06-01 至 2016-05-31
  • 项目状态:
    已结题

项目摘要

Understanding how the genome is read by the cellular machinery to allow the cell to survive is a major focus of biological research. One specific enzyme of the cellular machinery contains an unusual region of highly repetitive structure critical to gene expression. However the reason for having so many repetitions of the same structure or the specific function of individual repeats is currently unknown. This project will develop novel genetic tools to investigate the role of these repeats in controlling gene expression. The development and application of this approach will be performed in collaboration with Dr. Hiroaki Kato of the Shimane University School of Medicine in Japan, an expert in the control of gene expression by the cellular machinery. Results obtained from this work will drive further understanding of the role of protein repeats during gene expression in both healthy and sick cells. Progress through the transcription cycle is tightly regulated by the heptad repeats of the C-terminal domain of RNA polymerase II. These repeats, numbering 26 in yeast and 52 in humans, are post-translationally modified to recruit additional protein complexes to the site of transcription. This project will investigate specific stretches of repeats to understand if identical protein repeats can have unique functions in recruiting protein factors. Collaboration with Dr. Kato will provide access to the fission yeast model organism to complement previous studies in budding yeast. A new system for studying RNA polymerase II based upon a method used in budding yeast will be developed in fission yeast. The system will then be applied to study repeat-specific protein recruitment during heterochromatin silencing ? a pathway present in fission yeast (and shared with higher eukaryotes) but absent in budding yeast. This comparative approach will uncover additional spatial mechanisms of the C-terminal domain repeats in regulating the activity of RNA polymerase II. This NSF EAPSI award is funded in collaboration with the Japan Society for the Promotion of Science (JSPS).
了解细胞机器如何读取基因组以使细胞存活是生物学研究的主要焦点。细胞机器中的一种特定酶含有一个对基因表达至关重要的高度重复结构的不寻常区域。然而,相同结构的重复如此之多的原因或单个重复的特定功能目前尚不清楚。该项目将开发新的遗传工具来研究这些重复序列在控制基因表达中的作用。这种方法的开发和应用将与日本岛根大学医学院的Hiroaki Kato博士合作进行,Hiroaki Kato博士是通过细胞机制控制基因表达的专家。从这项工作中获得的结果将推动进一步了解蛋白质重复序列在健康和患病细胞中基因表达过程中的作用。通过转录周期的进展受到RNA聚合酶II的C-末端结构域的七肽重复序列的严格调控。这些重复序列,在酵母中编号为26,在人类中编号为52,在转录后被修饰以将额外的蛋白质复合物募集到转录位点。该项目将研究特定的重复序列,以了解相同的蛋白质重复序列是否在招募蛋白质因子方面具有独特的功能。与加藤博士的合作将提供裂变酵母模式生物,以补充以前在芽殖酵母中的研究。基于芽殖酵母中使用的方法,将在裂殖酵母中开发用于研究RNA聚合酶II的新系统。然后,该系统将被应用于研究重复特异性蛋白质募集过程中异染色质沉默?一种存在于分裂酵母中(和高等真核生物共有)但在芽殖酵母中不存在的途径。这种比较的方法将揭示额外的空间机制的C-末端结构域重复序列在调节RNA聚合酶II的活性。NSF EAPSI奖由日本科学促进会(JSPS)资助。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Michael Babokhov其他文献

DNA Instability Maintains the Repeat Length of the Yeast RNA Polymerase II C-terminal Domain*
DNA 不稳定性维持了酵母 RNA 聚合酶 II C 末端结构域的重复长度*
  • DOI:
  • 发表时间:
    2016
  • 期刊:
  • 影响因子:
    4.8
  • 作者:
    Summer Morrill;Alexandra E. Exner;Michael Babokhov;Bradley I. Reinfeld;S. Fuchs
  • 通讯作者:
    S. Fuchs
Tandem repeats drive variation of intrinsically disordered regions in budding yeast
串联重复驱动芽殖酵母中本质无序区域的变化
  • DOI:
    10.1101/339663
  • 发表时间:
    2018
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Michael Babokhov;Bradley I. Reinfeld;Kevin Hackbarth;Yotam Bentov;S. Fuchs
  • 通讯作者:
    S. Fuchs

Michael Babokhov的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

相似海外基金

The Platelet Transcriptome and Organ Failure After Injury: Discovering Molecular Biomarkers and Preventative Targets through Interrogating Novel RNA Modifications
血小板转录组和损伤后器官衰竭:通过研究新的 RNA 修饰发现分子生物标志物和预防靶点
  • 批准号:
    10711791
  • 财政年份:
    2023
  • 资助金额:
    $ 0.51万
  • 项目类别:
Discovering Novel Properties in Few-Layer MXenes Using Analytical, In-Situ Scanning Transmission Electron Microscopy
使用分析原位扫描透射电子显微镜发现少层 MXene 的新特性
  • 批准号:
    2309396
  • 财政年份:
    2023
  • 资助金额:
    $ 0.51万
  • 项目类别:
    Continuing Grant
Discovering novel approaches to combat obesity by manipulating diet composition and thermogenesis in brown fat
通过控制饮食成分和棕色脂肪的生热作用,发现对抗肥胖的新方法
  • 批准号:
    559954-2021
  • 财政年份:
    2022
  • 资助金额:
    $ 0.51万
  • 项目类别:
    Alexander Graham Bell Canada Graduate Scholarships - Doctoral
Discovering novel predictors of minimally verbal outcomes in autism through computational modeling
通过计算模型发现自闭症最低限度语言结果的新预测因素
  • 批准号:
    10521901
  • 财政年份:
    2022
  • 资助金额:
    $ 0.51万
  • 项目类别:
Methylotrophs: underexplored bacteria for discovering novel natural products and biochemistry
甲基营养菌:用于发现新型天然产物和生物化学的尚未开发的细菌
  • 批准号:
    10810046
  • 财政年份:
    2022
  • 资助金额:
    $ 0.51万
  • 项目类别:
Discovering novel microRNAs that regulate central insulin sensitivity
发现调节中枢胰岛素敏感性的新型 microRNA
  • 批准号:
    575921-2022
  • 财政年份:
    2022
  • 资助金额:
    $ 0.51万
  • 项目类别:
    Alexander Graham Bell Canada Graduate Scholarships - Master's
Computational analysis of whole genome sequence data for discovering novel risk genes of structural birth defects
全基因组序列数据的计算分析,以发现结构性出生缺陷的新风险基因
  • 批准号:
    10354418
  • 财政年份:
    2022
  • 资助金额:
    $ 0.51万
  • 项目类别:
Discovering novel genetic alterations in highly complicated genetic regions of pediatric brain tumors
在儿科脑肿瘤高度复杂的遗传区域中发现新的遗传改变
  • 批准号:
    22K19591
  • 财政年份:
    2022
  • 资助金额:
    $ 0.51万
  • 项目类别:
    Grant-in-Aid for Challenging Research (Exploratory)
Discovering novel p53 binding partners through pCAP therapeutic targeting
通过 pCAP 治疗靶向发现新型 p53 结合伴侣
  • 批准号:
    575151-2022
  • 财政年份:
    2022
  • 资助金额:
    $ 0.51万
  • 项目类别:
    University Undergraduate Student Research Awards
Computational analysis of whole genome sequence data for discovering novel risk genes of structural birth defects
全基因组序列数据的计算分析,以发现结构性出生缺陷的新风险基因
  • 批准号:
    10673600
  • 财政年份:
    2022
  • 资助金额:
    $ 0.51万
  • 项目类别:
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了