PIRE: Halting Environmental Antimicrobial Resistance Dissemination (HEARD)

PIRE：阻止环境抗菌素耐药性传播 (HEARD)

• 批准号: 1545756
• 负责人: Peter Vikesland
• 金额: $ 360万
• 依托单位: Virginia Polytechnic Institute and State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-10-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1545756&HistoricalAwards=false
• 关键词: PIRE; Halting; Environmental; Antimicrobial; Resistance

Antimicrobial resistance (AMR), which occurs when disease-causing organisms no longer respond to the drugs commonly used to treat them, is a worldwide public health crisis and as such has been proclaimed to be one of the greatest threats to human wellbeing of the 21st Century. Halting AMR is a complex task because natural background levels of AMR vary worldwide, there are many ways that humans impact AMR, and because natural and human impacts interact in different ways around the world to influence how multi-antimicrobial resistant "super-bugs" arise and are transmitted. Although substantial effort has focused on lessening hospital-derived resistance, the spread of AMR has continued to accelerate, thus creating new attention to diminishing the spread and/or transmission of AMR in the wastewater environment. Wastewater treatment plants are a logical focus because they serve as collection points for resistant organisms and antimicrobial compounds from a wide variety of sources (i.e., hospitals, industries, households) and they are potential breeding grounds for environmental dissemination of AMR. Antimicrobial drugs and other chemical stressors (e.g., heavy metals, biocides) regularly enter wastewater treatment plants and may select for resistant organisms, while also stimulating them to produce and share the DNA elements responsible for resistance. This PIRE project, Halting Environmental Antimicrobial Resistance Dissemination [HEARD], will 1) quantify how wastewater treatment processes affect different aspects of AMR (e.g., the antimicrobial drugs, AMR organisms, and the DNA elements underlying AMR) across a global transect of wastewater treatment plants, 2) determine how the characteristics of wastewater treatment plants and the receiving environment (e.g., river, lake, or pipe network) interact to affect the spread of AMR, and 3) develop and test novel approaches to stop the spread of AMR originating from wastewater treatment plants. The international team assembled for this PIRE project includes researchers from four U.S. institutions and six other countries (China, India, Philippines, Portugal, Sweden and Switzerland). The international dimensions of this project are essential because 1) the propagation of AMR is of global concern, 2) the use and disposal of antimicrobials and wastewater management practices differ significantly from one society to another, and 3) international research collaboration prepares U.S. students to be part of a globally engaged U.S. science and engineering workforce.Three overarching hypotheses drive HEARD: Hypothesis 1: wastewater treatment plant influents can be monitored to gauge the impacts of local antimicrobial use and disposal practices on the prevalence of resistant organisms and resistance elements. Hypothesis 2: A broad gradient of antimicrobial resistance elements and resistant bacteria are present in wastewater effluents across the globe. Hypothesis 3: wastewater treatment processes and receiving environments can be chosen or modified to mitigate the spread of antimicrobial resistance. To address these hypotheses and answer these questions we have developed a comprehensive research plan organized around three research thrusts: Thrust 1: Global Reconnaissance of Antimicrobial Drugs, Antibiotic Resistant Bacteria, and Resistance Element Fate During Wastewater Treatment; Thrust 2: The Relative Roles of Wastewater Treatment Plants and Receiving Environments in Resistance Dissemination; and Thrust 3: Advancing Wastewater Treatment Technologies for Antimicrobial Drug, Antibiotic Resistant Bacteria, and Resistance Element Removal. HEARD brings together four U.S. universities and ten international academic institutions. The project's initial focus will be to globally track and quantify the concentrations of a select group of target resistance elements (e.g., NDM-1, intI1, blaTEM, vanA, and sul1) within wastewater treatment plant influents and effluents in the U.S., Asia, and Europe. In parallel, the project members will utilize metagenomics to detect nontarget resistance elements and bacteria. The metagenomic information will then direct and refine future targeted sampling efforts across the global transect of field sites. To develop solutions to the threat of wastewater mediated resistance dissemination the team will examine both at field and laboratory scale how changes in wastewater treatment plant operational variables (e.g., F/M ratio, solids retention time, and aerobic/anaerobic conditions) affect both resistant bacteria and resistance elements. The project's international partners synergistically provide the U.S.-based PIRE students with intracultural context, international research experience with access to world-class collaborators and facilities, and unique expertise in antimicrobial resistance and the global threat of resistance dissemination.This award is co-funded by the Division of Chemical, Bioengineering, Environmental, and Transport Systems of NSF's Directorate for Engineering.

抗菌素耐药性（AMR）是指致病微生物不再对常用药物产生反应，是一种全球性的公共卫生危机，因此已被宣布为21世纪世纪对人类健康的最大威胁之一。阻止AMR是一项复杂的任务，因为AMR的自然背景水平在世界范围内各不相同，人类影响AMR的方式有很多，并且因为自然和人类的影响在世界各地以不同的方式相互作用，影响多重抗菌素耐药性“超级细菌”的产生和传播。 虽然大量的努力集中在减少医院来源的耐药性，AMR的传播继续加速，从而产生了新的关注，以减少AMR在废水环境中的传播和/或传播。废水处理厂是一个合乎逻辑的焦点，因为它们作为耐药生物和来自各种来源的抗微生物化合物的收集点（即，医院、工业、家庭），它们是AMR环境传播的潜在滋生地。抗微生物药物和其他化学应激物（例如，重金属、生物杀灭剂）定期进入废水处理厂，并可能选择抗性生物，同时也刺激它们产生和分享负责抗性的DNA元件。这个PIRE项目，停止环境抗菌素耐药性传播[HEARD]，将1）量化废水处理过程如何影响AMR的不同方面（例如，抗微生物药物、AMR生物体和AMR背后的DNA元件），2）确定废水处理厂和接收环境的特性（例如，河流、湖泊或管网）相互作用，影响AMR的传播，3）开发和测试新的方法，以阻止源自废水处理厂的AMR的传播。PIRE项目的国际团队包括来自四个美国机构和其他六个国家（中国，印度，菲律宾，葡萄牙，瑞典和瑞士）的研究人员。该项目的国际层面是必不可少的，因为1）AMR的传播是全球关注的问题，2）抗菌剂的使用和处置以及废水管理实践在不同社会之间存在显著差异，3）国际研究合作使美国学生成为全球参与的美国科学和工程劳动力的一部分。HEARD的三个主要假设：假设1：可以监测废水处理厂的流入物，以衡量当地抗菌剂的使用和处置做法对耐药生物体和耐药元素流行的影响。 假设2：在地球仪的废水排放物中存在广泛的抗微生物耐药元素和耐药细菌梯度。假设3：可以选择或修改废水处理工艺和接收环境，以减轻抗菌素耐药性的传播。为了解决这些假设和回答这些问题，我们制定了一个全面的研究计划，围绕三个研究重点组织：重点1：全球调查抗菌药物，抗生素耐药细菌和耐药元素在废水处理过程中的命运;重点2：废水处理厂和接收环境在耐药性传播中的相对作用;以及重点3：先进的废水处理技术，用于抗菌药物，抗生素耐药细菌和抗性元素的去除。HEARD汇集了四所美国大学和十个国际学术机构。该项目最初的重点将是在全球范围内跟踪和量化一组选定的目标耐药元素（例如，NDM-1、intI 1、blaTEM、vanA和sul 1）在美国污水处理厂流入物和流出物中的应用，亚洲和欧洲。与此同时，项目成员将利用宏基因组学来检测非靶向耐药元件和细菌。然后，宏基因组信息将指导和完善未来在全球实地样带中有针对性的采样工作。为了开发解决废水介导的抗性传播威胁的解决方案，该团队将在现场和实验室规模上研究废水处理厂操作变量（例如，F/M比、固体保留时间和好氧/厌氧条件）影响抗性细菌和抗性元件。 该项目的国际合作伙伴协同提供美国-该奖项由美国国家科学基金会工程理事会化学、生物工程、环境和运输系统部门共同资助。

项目成果

Comprehensive assessment of chemical residues in surface and wastewater using passive sampling, chemical, biological, and fish behavioral assays

使用被动采样、化学、生物和鱼类行为测定对地表和废水中的化学残留物进行综合评估

• DOI: 10.1016/j.scitotenv.2022.154176
• 发表时间: 2022
• 期刊: Science of The Total Environment
• 影响因子: 9.8
• 作者: Brunelle, Laura D.;Huang, Irvin J.;Angeles, Luisa F.;Running, Logan S.;Sirotkin, Howard I.;McElroy, Anne E.;Aga, Diana S.
• 通讯作者: Aga, Diana S.

Lectin-Modified Bacterial Cellulose Nanocrystals Decorated with Au Nanoparticles for Selective Detection of Bacteria Using Surface-Enhanced Raman Scattering Coupled with Machine Learning

• DOI: 10.1021/acsanm.1c02760
• 发表时间: 2022-01-07
• 期刊: ACS APPLIED NANO MATERIALS
• 影响因子: 5.9
• 作者: Rahman, Asifur;Kang, Seju;Vikesland, Peter J.
• 通讯作者: Vikesland, Peter J.

Complementing RNA Detection with Pharmaceutical Monitoring for Early Warning of Viral Outbreaks through Wastewater-Based Epidemiology

• DOI: 10.1021/acs.estlett.2c00259
• 发表时间: 2022-06-14
• 期刊: ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
• 影响因子: 10.9
• 作者: Halwatura, Lahiruni M.;Mclerran, Isabella S.;Aga, Diana S.
• 通讯作者: Aga, Diana S.

Surface-Enhanced Raman Spectroscopy of Bacterial Metabolites for Bacterial Growth Monitoring and Diagnosis of Viral Infection

细菌代谢物的表面增强拉曼光谱用于细菌生长监测和病毒感染诊断

• DOI: 10.1021/acs.est.1c02552
• 发表时间: 2021
• 期刊: Environmental Science & Technology
• 影响因子: 11.4
• 作者: Wang, Wei;Kang, Seju;Vikesland, Peter J.
• 通讯作者: Vikesland, Peter J.

One-step biosynthesis of a bilayered graphene oxide embedded bacterial nanocellulose hydrogel for versatile photothermal membrane applications

用于多功能光热膜应用的双层氧化石墨烯嵌入细菌纳米纤维素水凝胶的一步生物合成

• DOI: 10.1039/d1en00754h
• 发表时间: 2022
• 期刊: Environmental Science: Nano
• 作者: Divyapriya, Govindaraj;Rahman, Asifur;Leng, Weinan;Wang, Wei;Vikesland, Peter J.
• 通讯作者: Vikesland, Peter J.