CAREER: Testing The Predictability of Flower Color Evolution at a Phylogenetic Scale in the Petunieae Clade (Solanaceae)

职业：在矮牵牛科（茄科）的系统发育尺度上测试花色进化的可预测性

• 批准号: 1553114
• 负责人: Stacey Smith
• 金额: $ 95.45万
• 依托单位: University of Colorado at Boulder
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2024-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1553114&HistoricalAwards=false
• 关键词: CAREER; Testing; Predictability; Flower; Color

Evolutionary biologists have long recognized that species can evolve many different forms (phenotypes) using the same sets of genes. One explanation for this pattern is that new forms can arise by changes in gene regulation, that is, shifts in when and where those genes are active. Although regulatory differences are known to underlie changes in features as complex as eyes and wings, it remains unclear how broadly this regulatory hypothesis applies and whether, in some cases, changes in the genes themselves are more important. This project will address this question by tracing the evolutionary changes involved in flower color transitions across Petunieae, a group of 140 species that includes the ornamental petunias. The genes that produce floral pigments are well studied, making it possible to characterize the genetic changes associated with flower color evolution even at this broad scale. As a part of this work, the researchers will create and present color-themed activities to diverse groups of K-12 students and undergraduates in order to enhance teaching and learning about the connections between genes, pathways, and phenotypes across the tree of life.This project will test the role of regulatory evolution in the color diversification of Petunieae through three specific aims. The first aim will infer the history of flower color shifts in the clade using a new phylogeny constructed from transcriptomic data. Building on this phylogenetic framework, the second aim will use comparative methods and biochemical profiling to quantify the evolutionary relationship between changes in the expression of pigment pathway genes and color variation. Given the modular nature of the regulation of these genes, the third aim will test for correlated evolution of gene expression across the phylogeny. These aims will be carried out through collaborations with botanists from Brazil and Argentina, who will participate in research exchanges to foster the sharing of knowledge and techniques. At a local scale, the research activities will be integrated into outreach events as well as inquiry-based course modules for undergraduates and upward-bound high school students. These innovative teaching approaches will be coupled with new tools to assess learning gains and guide improvement.

进化生物学家很早就认识到，物种可以使用相同的基因集进化出许多不同的形式（表型）。 对这种模式的一种解释是，基因调控的变化可以产生新的形式，即这些基因活跃的时间和地点的变化。 尽管已知调控差异是眼睛和翅膀等复杂特征变化的基础，但目前尚不清楚这种调控假设的适用范围有多大，以及在某些情况下基因本身的变化是否更重要。该项目将通过追踪矮牵牛科（包括观赏矮牵牛在内的 140 个物种）花色转变所涉及的进化变化来解决这个问题。 产生花色素的基因得到了充分研究，使得即使在如此广泛的范围内也能够表征与花颜色进化相关的遗传变化。 作为这项工作的一部分，研究人员将创建并向不同群体的 K-12 学生和本科生展示颜色主题活动，以加强对生命树中基因、通路和表型之间联系的教学和学习。该项目将通过三个具体目标测试调节进化在矮牵牛颜色多样化中的作用。第一个目标是使用根据转录组数据构建的新系统发育来推断进化枝中花朵颜色变化的历史。在此系统发育框架的基础上，第二个目标将使用比较方法和生化分析来量化色素途径基因表达变化与颜色变化之间的进化关系。考虑到这些基因调控的模块化性质，第三个目标将测试整个系统发育中基因表达的相关进化。这些目标将通过与巴西和阿根廷的植物学家合作来实现，他们将参与研究交流，以促进知识和技术的共享。在地方范围内，研究活动将被纳入外展活动以及针对本科生和向上发展的高中生的探究式课程模块中。这些创新的教学方法将与新工具相结合，以评估学习成果并指导改进。

项目成果

Computational Modeling of Anthocyanin Pathway Evolution: Biases, Hotspots, and Trade-offs

花青素途径进化的计算模型：偏差、热点和权衡

• DOI: 10.1093/icb/icz049
• 发表时间: 2019
• 期刊: Integrative and Comparative Biology
• 影响因子: 2.6
• 作者: Wheeler, L. C.;Smith, S. D.
• 通讯作者: Smith, S. D.

Modelling pollinator and nonpollinator selection on flower colour variation

• DOI: 10.1111/1365-2745.14057
• 发表时间: 2023-04
• 期刊: Journal of Ecology
• 影响因子: 5.5
• 作者: B. Tenhumberg;A. Dellinger;Stacey D. Smith

Stepwise evolution of floral pigmentation predicted by biochemical pathway structure

• DOI: 10.1111/evo.13589
• 发表时间: 2018-12-01
• 期刊: EVOLUTION
• 影响因子: 3.3
• 作者: Ng, Julienne;Freitas, Loreta B.;Smith, Stacey D.
• 通讯作者: Smith, Stacey D.

Repeated evolution of a morphological novelty: a phylogenetic analysis of the inflated fruiting calyx in the Physalideae tribe (Solanaceae)

• DOI: 10.1002/ajb2.1242
• 发表时间: 2019-02-01
• 期刊: AMERICAN JOURNAL OF BOTANY
• 影响因子: 3
• 作者: Deanna, Rocio;Larter, Maximilian D.;Smith, Stacey D.
• 通讯作者: Smith, Stacey D.

Phylogenetics is the New Genetics (for Most of Biodiversity)

系统发育学是新的遗传学（对于大多数生物多样性而言）

• DOI: 10.1016/j.tree.2020.01.005
• 发表时间: 2020
• 期刊: Trends in Ecology & Evolution
• 影响因子: 16.8
• 作者: Smith, Stacey D.;Pennell, Matthew W.;Dunn, Casey W.;Edwards, Scott V.
• 通讯作者: Edwards, Scott V.