CSBR: Living Stock Collections: A "Biological Gold Mine" of plant natural products and pathways for biochemical and evolutionary studies

CSBR：活畜收藏：植物天然产物的“生物金矿”以及生化和进化研究的途径

• 批准号: 1561572
• 负责人: Jennifer Normanly
• 金额: $ 31.59万
• 依托单位: University of Massachusetts Amherst
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1561572&HistoricalAwards=false
• 关键词: CSBR; Living; Stock; Collections; Biological

An award is made to the University of Massachusetts Amherst to improve a large Plant Cell Culture Library (PCCL) that was recently donated to the university from industry, and make it available to the research community. The PCCL includes over 2200 unique plant species, the largest and most diverse collection of plant cell cultures in the world (representing approximately 69% of plant orders) and is the only such collection in the western hemisphere. The PCCL is a unique and valuable source of novel natural products and the genetic information required for their biosynthesis. Significantly, the availability of the PCCL to the academic research community and to industry has the potential for broad societal impact through the discovery and development of new compounds with applications in ecological sustainability (green chemistry, renewable resources), agriculture (prevention of crop loss with novel anti-microbial or anti-fungal agents, nutritional enhancement of food and feed crops) and human health and wellness. Additionally, the PCCL provides a valuable resource for undergraduate education and outreach as a source of material with which to engage students in the vast genetic and biochemical diversity of plants. Specifically, this project will streamline cell culture maintenance protocols and optimize procedures for rapid culture scale-up and dissemination to the research community. The PCCL database will be modernized, a web interface for the research community deployed, and a sustainable model for maintaining and distributing PCCL materials will be developed. The approach will improve the development of cold storage conditions that will reduce the labor and cost of maintaining the living cultures by slowing growth to increase the time between transfers. Cryopreservation methods will be tested to optimize the transfer of as many living cultures as possible to a cryopreserved state. Cryopreserved materials will be duplicated to be deposited in the National Center for Genetic Resources Preservation, a USDA facility in Fort Collins. Culture media will be evaluated to reduce the amount of media required for culture maintenance. Finally, an updated PCCL taxonomy will be integrated into a web-based interface to improve accessibility of the PCCL (pcc.library.umass.edu).

马萨诸塞大学阿默斯特分校被授予一个奖项，以改进最近从工业界捐赠给该大学的大型植物细胞培养库(PCCL)，并使其可供研究界使用。PCCL包括2200多个独特的植物物种，是世界上最大和最多样化的植物细胞培养集合(约占植物目的69%)，也是西半球唯一的此类集合。PCCL是新的天然产物及其生物合成所需遗传信息的独特而宝贵的来源。值得注意的是，通过发现和开发在生态可持续性(绿色化学、可再生资源)、农业(使用新型抗微生物或抗真菌制剂防止作物损失、提高食品和饲料作物的营养)以及人类健康和健康方面的新化合物，PCCL对学术研究界和工业具有广泛的社会影响。此外，PCCL为本科生教育和推广提供了宝贵的资源，作为让学生参与植物广泛的遗传和生物化学多样性的材料来源。具体地说，该项目将简化细胞培养维护方案，并优化快速扩大培养规模并向研究界传播的程序。PCCL数据库将实现现代化，为研究界部署一个网络界面，并将制定一个维护和分发PCCL材料的可持续模式。该方法将改善冷藏条件的发展，通过减缓增长以增加两次转移之间的时间，从而减少维持活的文化的劳动力和成本。将对低温保存方法进行测试，以优化将尽可能多的活培养物转移到低温保存状态。冷冻保存的材料将被复制保存在国家遗传资源保护中心，这是美国农业部在柯林斯堡的设施。将对培养基进行评估，以减少维持培养所需的基质量。最后，最新的PCCL分类将纳入一个基于网络的界面，以改善PCCL(pcc.Library.umass.edu)的可及性。