Expanding the Use of Liquid Chromatography Through Active Temperature Control

通过主动温度控制扩大液相色谱的使用

• 批准号: 1608757
• 负责人: Stephen Weber
• 金额: $ 42.25万
• 依托单位: University of Pittsburgh
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1608757&HistoricalAwards=false
• 关键词: Expanding; Use; Liquid; Chromatography; Through

This project is funded by the Chemical Measurement and Imaging Program of the Division of Chemistry at the National Science Foundation. Professor Stephen Weber and colleagues at the University of Pittsburgh address limitations of liquid chromatography. The team is improving the ability of liquid chromatography techniques to distinguish one substance from another, as well as improving the ability to measure trace concentrations in a variety of samples. Chromatography is based on the principle of partitioning, the tendency of a substance to prefer an oily substance to water-based solution. The outcomes of this project include obtaining more and better information from samples with lower cost and lower environmental burden than has been previously realized. Collaborations with scientists in the fields of proteomics (for example, health, pharmaceuticals, and biochemistry research) and two-dimensional liquid chromatography (for example, pharmaceuticals, chemical industry, agriculture, and the environment) demonstrate the effectiveness of the project goals. The broader impacts are evidenced in that improvements in chromatographic processes that are useful across many fields and applications. Liquid chromatography is used in virtually every industry, for example, environmental, pharmaceutical, nutritional, forensic, toxicology, polymers, chemicals, and cosmetics. A Girl Scout merit badge project is also being developed by Dr. Weber to explore chromatography methods and its uses.The project uses temperature control of the column to achieve greater solute focusing and to control retention in a predictable way. Retention enthalpies, which control the sensitivity of a particular compound to changes in temperature, are determined for a number of related organic compounds. These data are used to determine molecular fragment enthalpies. The temperature dependence of the retention of novel compounds are then predicted. Software to predict chromatographic behavior in the face of changing column temperature aids in chromatographic method development. The temperature control, when used to aid solute focusing, significantly improves concentration detection limits.

本项目由美国国家科学基金化学学部化学测量与成像项目资助。匹兹堡大学的斯蒂芬·韦伯教授及其同事解决了液相色谱法的局限性。该团队正在提高液相色谱技术的能力，以区分一种物质与另一种物质，以及提高测量各种样品中痕量浓度的能力。色谱法是基于分配原理，即物质倾向于油性物质而不是水基溶液。该项目的成果包括从样品中获得更多和更好的信息，成本更低，环境负担更低，比以前实现的。与蛋白质组学（例如，健康、制药和生物化学研究）和二维液相色谱（例如，制药、化学工业、农业和环境）领域的科学家的合作证明了项目目标的有效性。更广泛的影响在色谱过程的改进中得到证明，这些改进在许多领域和应用中都是有用的。液相色谱法几乎用于每个行业，例如，环境，制药，营养，法医，毒理学，聚合物，化学品和化妆品。韦伯博士还在开发一个女童军荣誉徽章项目，以探索色谱法及其用途。该项目使用温度控制柱来实现更大的溶质聚焦，并以可预测的方式控制保留。保留焓控制特定化合物对温度变化的敏感性，对许多相关的有机化合物是确定的。这些数据用于确定分子片段焓。然后预测了新化合物保留的温度依赖性。软件预测色谱行为在面对变化的柱温有助于色谱方法的发展。温度控制，当用来帮助溶质聚焦，显著提高浓度检测限。