Unraveling the genetic architecture of winter hardiness and quality traits in durum by genome wide and candidate gene based association mapping

通过全基因组和基于候选基因的关联作图揭示硬粒小麦抗寒性和品质性状的遗传结构

• 批准号: 226048836
• 负责人: Professor Dr. Friedrich Longin
• 金额: --
• 依托单位: Landessaatzuchtanstalt (720)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2012
• 资助国家: 德国
• 起止时间: 2011-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/226048836?language=en
• 关键词: Unraveling; genetic; architecture; winter; hardiness

Durum wheat is mainly grown as a summer crop. An introduction of a winter form failed until now due to the difficulty to combine winter hardiness with required process quality. Winter hardiness is a complex trait, but in most regions the frost tolerance is decisive. Thereby a major QTL, which was found in T. monococcum, T.aestivum, H. vulgare and S.cereale on chromosome 5, seems especially important. With genotyping by sequencing it is now possible to make association mapping based on very high dense marker maps, which delivers new possibilities to detect main and epistatic effects. Furthermore, new sequencing techniques allow candidate gene based association mapping. The main aim of the project is to unravel the genetic architecture of frost tolerance and quality traits in durum. Thereby, the objectives are to (1) determine the genetic variance, heritability and correlations among frost tolerance and quality traits, (2) examine linkage disequilibrium and population structure, (3) investigate sequence polymorphism at candidate genes for frost tolerance, and (4) perform candidate gene based and genome wide association mapping.

硬质小麦主要作为夏季作物种植。到目前为止，由于很难将冬季抗寒性与所需的工艺质量结合起来，引入冬季形式失败了。耐寒性是一个复杂的特征，但在大多数地区，耐霜性是决定性的。因此，在5号染色体上发现的一个主效QTL显得尤为重要，该QTL位于单粒小麦、普通小麦和麦粒小麦中。通过测序进行基因分型，现在可以基于非常高密度的标记图谱进行关联作图，这为检测主效应和上位性效应提供了新的可能性。此外，新的测序技术允许基于候选基因的关联映射。该项目的主要目的是揭示硬质植物耐霜性和品质性状的遗传结构。因此，目标是(1)确定耐霜性和品质性状之间的遗传方差、遗传力和相关性，(2)检验连锁不平衡和群体结构，(3)研究耐霜性候选基因的序列多态性，(4)进行基于候选基因和全基因组的关联定位。

项目成果

Combining frost tolerance, high grain yield and good pasta quality in durum wheat

• DOI: 10.1111/pbr.12064
• 发表时间: 2013-08-01
• 期刊: PLANT BREEDING
• 影响因子: 2
• 作者: Longin, Carl Friedrich Horst;Sieber, Alisa-Naomi;Reif, Jochen C.
• 通讯作者: Reif, Jochen C.

Vitreosity, its stability and relationship to protein content in durum wheat

硬粒小麦的玻璃体度、稳定性及其与蛋白质含量的关系

• DOI: 10.1016/j.jcs.2014.10.008
• 发表时间: 2015
• 期刊: Journal of Cereal Science
• 影响因子: 3.8
• 作者: Sieber;T. Würschum;C.F.H. Longin
• 通讯作者: C.F.H. Longin

The potential of genomic‐assisted breeding to improve Fusarium head blight resistance in winter durum wheat

基因组辅助育种提高冬硬粒小麦赤霉病抗性的潜力

• DOI: 10.1111/pbr.12515
• 发表时间: 2017
• 期刊: Plant Breeding
• 影响因子: 2
• 作者: Miedaner;A.-N. Sieber;H. Desaint;H. Bürstmayr;T. Würschum;C.F.H. Longin
• 通讯作者: C.F.H. Longin

Evaluation of a semi‐controlled test as a selection tool for frost tolerance in durum wheat (Triticum durum)

半对照试验作为硬粒小麦（Triticum durum）抗冻性选择工具的评估

• DOI: 10.1111/pbr.12181
• 发表时间: 2014
• 期刊: Plant Breeding
• 影响因子: 2
• 作者: Sieber;T. Würschum;C.F.H. Longin
• 通讯作者: C.F.H. Longin

Copy number variation of CBF-A14 at the Fr-A2 locus determines frost tolerance in winter durum wheat

Fr-A2基因座CBF-A14的拷贝数变异决定了冬季硬粒小麦的抗冻性

• DOI: 10.1007/s00122-016-2685-3
• 发表时间: 2016
• 期刊: Theoretical and Applied Genetics
• 影响因子: 5.4
• 作者: Sieber;C.F.H. Longin;W.L. Leiser;T. Würschum
• 通讯作者: T. Würschum