Collaborative Research: Parental Effects, Telomere Dynamics, and the Cross-generational Consequences of Stressors

合作研究：父母效应、端粒动力学和压力源的跨代后果

• 批准号: 1656194
• 负责人: Britt Heidinger
• 金额: $ 61.02万
• 依托单位: North Dakota State University Fargo
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1656194&HistoricalAwards=false
• 关键词: Collaborative; Research; Parental; Effects; Telomere

Stress experienced by parents often has long-lasting effects on offspring. One intriguing but untested possibility is that stressors in parents alter their genes and these changes may then influence offspring. This project investigates this possibility using controlled experiments and field surveys in a bird system. The results will contribute to the understanding of mechanisms and consequences of cross-generation effects of environmental stressors, which is critical to understanding the long-term impacts of environmental change on organisms. This project incorporates students at several levels, from high school to post-graduate, who will be embedded in a mentoring network. This experience will give them thorough training in all aspects of being a scientist, including proposal writing, conducting research in the field and the lab, giving presentations, and preparing publications. Students will be actively recruited through Research to Improve Diversity and Education (RIDE), an organization that is dedicated to increasing the number of minorities in science, including local Tribal colleges in North Dakota. Moreover, the research team will also develop inquiry-based learning modules using data from the study for high school and college students that will be presented at Tribal colleges. This collaborative research project will assess the importance of an alternative mechanism of inheritance, direct effects of stressors on the DNA (telomeres) in parental gametes that are inherited by offspring. This mechanism would be a new route by which parental stress can impact offspring, and the study will document the lifetime effects on fitness of both this direct pathway and any indirect ones. These results will significantly expand the understanding of the mechanisms and functional consequences of cross-generational effects of environmental stressors, which is likely to be critical for predicting the long-term impacts of environmental change. In addition, this research will provide novel information about telomere length and loss rate in a wild system. Telomeres are increasingly used as a measure of biological aging in physiological ecology, yet there is still a lack of basic knowledge about the inheritance patterns and fitness consequences of variation in telomere length and loss rate in free-living organisms. The integrative approach employed spans molecular, hormonal and whole organism responses and in doing so will offer unique insight into the mechanistic underpinnings and functional consequences of stress exposure across generations. The results will add to our ability to assess how increasing stress levels will impact age-structured population dynamics and the potential for mitigating the impacts of early adversity for individuals and their descendants.

父母经历的压力往往对后代产生长期影响。一个有趣但未经证实的可能性是，父母的压力改变了他们的基因，这些变化可能会影响后代。本项目通过在鸟类系统中进行控制实验和实地调查来研究这种可能性。研究结果将有助于理解环境压力源跨代效应的机制和后果，这对于理解环境变化对生物体的长期影响至关重要。该项目包括从高中到研究生的几个层次的学生，他们将被纳入一个辅导网络。这种经验将使他们在作为一个科学家的各个方面得到全面的培训，包括提案写作，在现场和实验室进行研究，演讲和准备出版物。学生将通过研究，以提高多样性和教育（骑），一个致力于增加少数民族在科学，包括在北达科他州当地部落学院的数量组织积极招募。此外，研究小组还将利用研究数据为高中和大学生开发基于探究的学习模块，这些模块将在部落学院展示。这项合作研究项目将评估替代遗传机制的重要性，即压力对后代遗传的亲本配子中DNA（端粒）的直接影响。这一机制将成为父母压力影响后代的新途径，该研究将记录这一直接途径和任何间接途径对适应性的终身影响。这些结果将大大扩大对环境压力源的跨代影响的机制和功能后果的理解，这可能是预测环境变化的长期影响的关键。此外，这项研究将提供有关野生系统中端粒长度和丢失率的新信息。在生理生态学中，端粒越来越多地被用作生物衰老的量度，然而，对于自由生活的生物体中端粒长度和损失率的变化的遗传模式和适应性后果仍然缺乏基本的知识。所采用的综合方法跨越分子，激素和整个生物体的反应，并在这样做将提供独特的洞察力的机制基础和跨代压力暴露的功能后果。这些结果将增加我们评估压力水平增加将如何影响年龄结构人口动态的能力，以及减轻个人及其后代早期逆境影响的潜力。

项目成果

Parental age does not influence offspring telomeres during early life in common gulls (Larus canus)

父母年龄并不影响普通海鸥（Larus canus）早期生命中的后代端粒

• DOI: 10.1111/mec.15905
• 发表时间: 2021
• 期刊: Molecular ecology
• 影响因子: 4.9
• 作者: Sepp, T.;Meitern, R;Heidinger, B.;Noreikiene, K.;Rattiste, K.;Horak, P.;Saks, L.;Kittilson, J.;Urvik, J.;Giraudeau, M.
• 通讯作者: Giraudeau, M.

Longer telomeres during early life predict higher lifetime reproductive success in females but not males

• DOI: 10.1098/rspb.2021.0560
• 发表时间: 2021-05
• 期刊: Proceedings of the Royal Society B
• 作者: B. Heidinger;Aurelia C. Kucera;J. Kittilson;D. Westneat

Intergenerational effects on offspring telomere length: interactions among maternal age, stress exposure and offspring sex

对后代端粒长度的代际影响：母亲年龄、压力暴露和后代性别之间的相互作用

• DOI: 10.1098/rspb.2019.1845
• 发表时间: 2019
• 期刊: Proceedings of the Royal Society B: Biological Sciences
• 作者: Marasco, Valeria;Boner, Winnie;Griffiths, Kate;Heidinger, Britt;Monaghan, Pat
• 通讯作者: Monaghan, Pat

Introduction to the Symposium: Stress Phenotype: Linking Molecular, Cellular, and Physiological Stress Responses to Fitness

研讨会简介：压力表型：将分子、细胞和生理压力反应与健身联系起来

• DOI: 10.1093/icb/icz098
• 发表时间: 2019
• 期刊: Integrative and Comparative Biology
• 影响因子: 2.6
• 作者: Heidinger, Britt J.;Wada, Haruka
• 通讯作者: Wada, Haruka

Early‐breeding females experience greater telomere loss

早期繁殖的雌性会经历更大的端粒损失

• DOI: 10.1111/mec.14952
• 发表时间: 2019
• 期刊: Molecular Ecology
• 影响因子: 4.9
• 作者: Graham, Jessica L.;Bauer, Carolyn M.;Heidinger, Britt J.;Ketterson, Ellen D.;Greives, Timothy J.
• 通讯作者: Greives, Timothy J.