Development of a LED-enabled biosensor for the detection of BNP biomarkers based on flexible platform

由于低成本和大规模制造能力，用于医疗应用的实时机械柔性生物传感器成为当前工作的重点。提出的新诊断方法将使用显示设备来检测指示心力衰竭的重要生物标志物。如果成功证明，拟议的技术将导致开发灵活和可穿戴的生物传感器，具有有效的可制造性，可以直接向佩戴者提供心脏状态的实时评估。拟议研究的总体目标是开发一种柔性基板上的发光显示（LED）生物传感器，可用于检测B型利钠肽（BNP），一种心血管疾病的生物标志物，以及其他生物标志物。灵活和实时的诊断设备是有吸引力的，因为它们的简单性，快速响应，和低制造成本。建议使用二维导电聚合物在柔性平台上制作LED使能的生物传感器，用于实时分析。首先，基于聚苯胺的生物传感器和柔性基板上的LED器件将被集成，以实现原位测量。 其次，将使用临床人血清样本进行表征和检测。抗原结合后，LED将发出红光，其强度与BNP浓度成比例。假设漏电流将与BNP浓度直接相关，因此它可以提供人血清中BNP的定量。心脏病患者的临床血液样本将从匹兹堡大学医学中心（UPMC）获得。拟议的研究可能会成为诊断生物标志物的重要新工具，从而为公共卫生和诊断技术做出重大贡献。

A real-time mechanically flexible biosensor for medical applications is the focus of the current effort due to low-cost and mass manufacturing capability. The proposed new diagnostic approach will use a display device for detecting an important biomarker that indicates heart failure. If successfully demonstrated, the proposed technology will lead to the development of flexible and wearable biosensors with effective manufacturability that can provide real-time assessment of heart status directly to the wearer.The overall goal of the proposed research is to develop a light-emitted-display (LED)-enabled biosensor on flexible substrates that can be used to detect B-type Natriuretic Peptide (BNP), a cardiovascular disease biomarker, as well as other biomarkers. Flexible and real-time diagnostic devices are attractive because of their simplicity, fast response, and low manufacturing cost. It is proposed to use a 2-dimensional conducting polymer to fabricate an LED-enabled biosensor on the flexible platform for real-time analysis. First, polyaniline-based biosensor and a LED device on the flexible substrate will be integrated for enabling in situ measurement. Second, characterization and tests will be performed using clinical human serum samples. Upon antigen binding, the LED will emit red light whose intensity is proportional to BNP concentration. It is hypothesized that the drain current will be directly correlated to BNP concentrations, thus it can provide for quantification of BNP in human serum. Clinical blood samples from heart patients will be acquired from the University of Pittsburgh Medicine Center (UPMC). The proposed investigation can potentially lead to an important new tool for diagnosing biomarkers, resulting in significantly contributing to public health and diagnostic technology.