Development of a LED-enabled biosensor for the detection of BNP biomarkers based on flexible platform

由于低成本和大规模制造能力，用于医疗应用的实时机械柔性生物传感器是当前努力的焦点。提出的新诊断方法将使用显示设备来检测指示心力衰竭的重要生物标志物。如果成功演示，所提出的技术将导致柔性和可穿戴生物传感器的发展，具有有效的可制造性，可以直接向佩戴者提供心脏状态的实时评估。该研究的总体目标是开发一种基于柔性基底的发光显示（LED）生物传感器，可用于检测b型利钠肽（BNP），一种心血管疾病生物标志物，以及其他生物标志物。灵活的实时诊断设备因其简单、快速响应和低制造成本而具有吸引力。提出利用二维导电聚合物在柔性平台上制造led生物传感器，用于实时分析。首先，基于聚苯胺的生物传感器和柔性衬底上的LED器件将集成在一起，以实现原位测量。其次，将使用临床人血清样本进行表征和测试。抗原结合后，LED发出红光，其强度与BNP浓度成正比。推测引流电流与BNP浓度有直接关系，可用于定量测定人血清中BNP的含量。心脏病患者的临床血液样本将从匹兹堡大学医学中心（UPMC）获得。拟议的研究可能会导致诊断生物标志物的重要新工具，从而对公共卫生和诊断技术做出重大贡献。

A real-time mechanically flexible biosensor for medical applications is the focus of the current effort due to low-cost and mass manufacturing capability. The proposed new diagnostic approach will use a display device for detecting an important biomarker that indicates heart failure. If successfully demonstrated, the proposed technology will lead to the development of flexible and wearable biosensors with effective manufacturability that can provide real-time assessment of heart status directly to the wearer.The overall goal of the proposed research is to develop a light-emitted-display (LED)-enabled biosensor on flexible substrates that can be used to detect B-type Natriuretic Peptide (BNP), a cardiovascular disease biomarker, as well as other biomarkers. Flexible and real-time diagnostic devices are attractive because of their simplicity, fast response, and low manufacturing cost. It is proposed to use a 2-dimensional conducting polymer to fabricate an LED-enabled biosensor on the flexible platform for real-time analysis. First, polyaniline-based biosensor and a LED device on the flexible substrate will be integrated for enabling in situ measurement. Second, characterization and tests will be performed using clinical human serum samples. Upon antigen binding, the LED will emit red light whose intensity is proportional to BNP concentration. It is hypothesized that the drain current will be directly correlated to BNP concentrations, thus it can provide for quantification of BNP in human serum. Clinical blood samples from heart patients will be acquired from the University of Pittsburgh Medicine Center (UPMC). The proposed investigation can potentially lead to an important new tool for diagnosing biomarkers, resulting in significantly contributing to public health and diagnostic technology.