Collaborative Research: The evolutionary mechanics of hybridization across a primate radiation

合作研究：灵长类辐射杂交的进化机制

• 批准号: 1718339
• 负责人: Bruce Grant
• 金额: $ 13.51万
• 依托单位: New York University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-07-15 至 2024-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1718339&HistoricalAwards=false
• 关键词: Collaborative; Research; evolutionary; mechanics; hybridization

This is an investigation of whether hybridization between closely-related species has been a significant force in the evolution of primates. Recent research has uncovered that up to 5% of the DNA of many modern humans originated from ancient interbreeding with Neanderthal populations, raising the broader question of whether a species' genetic makeup includes genes brought together through occasional episodes of hybridization. In the present study, the genomes of a group of monkey species will be compared to determine whether segments of DNA have been transferred between species over evolutionary time. The project will broaden participation of groups under-represented in science through a partnership with the McNair Scholars Program (a federal program that identifies promising undergraduates who are first-generation, low-income and/or under-represented in PhD programs) and by working with other organizations that focus on similar groups of high school students, such as the Harlem Children's Society, BridgeUp:STEM, and ARISE in New York City. The project will also enhance infrastructure for research by making all custom-written software freely available on the internet and by presenting such software in workshops at the annual conference of H3ABioNet, a pan-African bioinformatics group. Hybridization has been reported among numerous species with the Cercopithecins. Thus, if historical interbreeding indeed leaves its mark on a species' genome, such evidence should be readily identified in these animals and serve as a model system for investigating hybridization in primates. Two central questions will be addressed. First, are genes more likely to be exchanged between closely related species (e.g., within a genus), or species with deep evolutionary separation (e.g., different genera)? The former scenario allows for a higher frequency of hybridization due to greater overall biological similarity, while the latter scenario allows for more variant, and possibly more advantageous, forms of genes to evolve and transfer between species. Second, will genes exchanged through hybridization be unique, or will patterns of genes with similar functions be found to repeatedly cross between species? If the latter, such patterns will reveal a more general pattern by identifying genes that are likely the essential drivers of the evolutionary process in primates. "Consensus" evolutionary relationships among these monkey species will be derived from an analysis of the total non-coding DNA sequence; this amounts to the majority of the genome and reflects the "true" set of evolutionary relationships. Coding sequences will then be analyzed individually and compared across all possible pairs of species; some sequences will follow the consensus pattern, but those that do not will likely reflect genes exchanged through hybridization. A series of statistical calculations will measure the probability of such genes being correctly identified as transfers via hybridization.

这是一个调查是否密切相关的物种之间的杂交一直是灵长类动物进化的重要力量。最近的研究发现，许多现代人高达5%的DNA起源于古代与尼安德特人的杂交，这提出了一个更广泛的问题，即一个物种的基因组成是否包括通过偶尔的杂交而聚集在一起的基因。在本研究中，将比较一组猴子物种的基因组，以确定DNA片段是否在进化过程中在物种之间转移。该项目将通过与麦克奈尔学者计划（一个联邦计划，确定有前途的本科生谁是第一代，低收入和/或在博士课程中代表性不足）的伙伴关系，并通过与其他组织，重点关注类似的高中学生群体，如哈莱姆儿童协会，桥：干，和ARISE在纽约市的合作，扩大在科学中代表性不足的群体的参与。该项目还将通过在互联网上免费提供所有定制编写的软件，并在泛非生物信息学小组H3 ABioNet年度会议的讲习班上介绍这些软件，加强研究基础设施。许多物种都曾与长尾猴属的物种发生杂交。因此，如果历史上的杂交确实在一个物种的基因组上留下了印记，那么这些证据应该很容易在这些动物中找到，并作为研究灵长类杂交的模型系统。将讨论两个核心问题。首先，基因是否更有可能在密切相关的物种之间交换（例如，属内），或具有深层进化分离的物种（例如，不同的属）？前一种情况允许更高频率的杂交，由于更大的整体生物学相似性，而后一种情况允许更多的变异，可能更有利的，基因的形式在物种之间进化和转移。第二，通过杂交交换的基因是唯一的，还是具有相似功能的基因模式会在物种之间重复杂交？如果是后者，这种模式将通过识别可能是灵长类动物进化过程基本驱动力的基因来揭示更普遍的模式。这些猴子物种之间的“一致”进化关系将来自对总的非编码DNA序列的分析;这相当于基因组的大部分，反映了“真实”的进化关系。然后将单独分析编码序列，并在所有可能的物种对中进行比较;一些序列将遵循共有模式，但那些不遵循的序列可能反映通过杂交交换的基因。一系列的统计计算将测量这些基因被正确鉴定为通过杂交转移的概率。

项目成果

The impacts of mating system on sperm gene evolution in primates

交配系统对灵长类动物精子基因进化的影响

• 发表时间: 2022
• 期刊: American journal of biological anthropology
• 作者: Decamp, RM;Trujillo, AE;Tilton, KM;Blair, ME;Pozzi, L;Disotell, TR;Tosi, AJ;Burrell, AS;Bergey, CM
• 通讯作者: Bergey, CM

Adaptive evolution of Plasmodium-associated genes in primates

灵长类动物疟原虫相关基因的适应性进化

• 发表时间: 2022
• 期刊: American journal of biological anthropology
• 作者: Tilton, KM;Trujillo, AE;Decamp, RM;Blair, ME;Pozzi, L;Disotell, TR;Tosi, AJ;Burrell, AS;Bergey, CM
• 通讯作者: Bergey, CM